A bioretention system is a low-impact and sustainable treatment facility for treating urban stormwater runoff. To meet or maintain a consistently satisfactory performance, especially in terms of increasing nitrogen removal efficiency, the introduction of a submerged (anoxic) zone (SZ) combined with a module-based carbon source (C) has been recommended. This study investigated the removal of nitrogen (N), phosphorus (P) and heavy metals with a retrofitted bioretention system. A significant (p < 0.05) removal enhancement of N as well as total phosphorus (TP) was observed, in the mesocosms with additions of exogenous carbon as opposed to those without such condition. However, even in the mesocosm with SZ alone (without exogenous C), TP removal showed significant enhancement. With regard to the effects of SZ depth on nutrient removal, the results showed that the removal of both N and P in module with a shallow SZ (200 mm) showed significant enhancement compared to that in module with a deep SZ (300 mm). Removal efficiencies greater than 93% were observed for all three heavy metals tested (Cu, Pb, and Zn) in all mesocosms, even in the bioretention module without an SZ or plants, and it indicated that adsorption by the filtration media itself is probably the most important removal mechanism. Only Cu (but not Pb or Zn) showed significantly enhanced removal in module with an SZ as compared to those without an SZ. Carbon source played a minor role in metal removal as no significant (p > 0.05) improvement was observed in module with C as compared to that without C. Based on these results, the incorporation of SZ with C in stormwater biofilters is recommended.of water irreversibly degraded [3,4]. Bioretention cells (BCs) integrated runoff retention areas, is a critical component of Low Impact Development (LID) practices receiving high attention as "green storm infrastructure" to manage storm runoff in a decentralized and source management approach [5,6]. BCs have gained popularity because of its design flexibility in terms of size and location, appealing landscape aesthetics, and hydrological performance for reduction of pollutants [7,8]. Previous studies have demonstrated that BCs implemented in relatively small catchments effectively improve both water quantity and quality in response to frequent storm events [9][10][11].In the vegetated filter media, pollutants from storm runoff can be removed through a variety of mechanisms, including physical, chemical and biological processes [12][13][14], and its quality is further enhanced by plant uptake and biological activities in the rhizosphere [15,16]. In recent years, research has shown that BCs can effectively improve water quantity and remove suspended solids [17,18], nutrients [19][20][21], and heavy metals [22,23]. However, it is difficult to meet or maintain a consistently satisfactory performance for reducing nitrogen due to a lack of effective denitrification [24,25].Since it is difficult to achieve consistent high nitrogen removal in standard stormwater bioretention s...