Adaptive biosensing platform using immune cell-based nanovesicles for food allergen detection

Jeong, Jinyoung; Kim, So-ong; Bang, Sunwoo; Choi, Young-Hee; Shin, Jaikwang; Choi, Danmin; Lee, Sang-Eun; Park, Tai Hyun; Hong, Seunghun

doi:10.1016/j.bios.2022.114914

Cited by 7 publications

(3 citation statements)

References 38 publications

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“…Most importantly, our IgE-EsAbs-based sELISA can specifically recognize IgE epitopes of Ara h 2, which makes it able to detect Ara h 2 allergenic residues and with the potential to measure Ara h 2 IgE-binding variations in processed foods ( 18 , 30 ). As shown in Supplementary Table S3 , the only analytical method that detects Ara h 2 IgE epitopes and with a significantly lower LOD is the rat basophilic leukemia (RBL-2H3) immune cell-based biosensing platform, with a LOD of 0.1 fmol/L (~0.002 ppb) ( 39 ). However, given that this sensor-based analytical technique requires cells culture, IgE antibodies to trigger an immunoreaction, and specialized knowledge, the IgE-EsAbs-based sELISA may be a more suitable method when lower costs and less complexity are desired.…”

Section: Resultsmentioning

confidence: 99%

A novel IgE epitope-specific antibodies-based sandwich ELISA for sensitive measurement of immunoreactivity changes of peanut allergen Ara h 2 in processed foods

Yan,

Li,

Long

et al. 2024

Front. Nutr.

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BackgroundPeanut is an important source of dietary protein for human beings, but it is also recognized as one of the eight major food allergens. Binding of IgE antibodies to specific epitopes in peanut allergens plays important roles in initiating peanut-allergic reactions, and Ara h 2 is widely considered as the most potent peanut allergen and the best predictor of peanut allergy. Therefore, Ara h 2 IgE epitopes can serve as useful biomarkers for prediction of IgE-binding variations of Ara h 2 and peanut in foods. This study aimed to develop and validate an IgE epitope-specific antibodies (IgE-EsAbs)-based sandwich ELISA (sELISA) for detection of Ara h 2 and measurement of Ara h 2 IgE-immunoreactivity changes in foods.MethodsDEAE-Sepharose Fast Flow anion-exchange chromatography combining with SDS-PAGE gel extraction were applied to purify Ara h 2 from raw peanut. Hybridoma and epitope vaccine techniques were employed to generate a monoclonal antibody against a major IgE epitope of Ara h 2 and a polyclonal antibody against 12 IgE epitopes of Ara h 2, respectively. ELISA was carried out to evaluate the target binding and specificity of the generated IgE-EsAbs. Subsequently, IgE-EsAbs-based sELISA was developed to detect Ara h 2 and its allergenic residues in food samples. The IgE-binding capacity of Ara h 2 and peanut in foods was determined by competitive ELISA. The dose-effect relationship between the Ara h 2 IgE epitope content and Ara h 2 (or peanut) IgE-binding ability was further established to validate the reliability of the developed sELISA in measuring IgE-binding variations of Ara h 2 and peanut in foods.ResultsThe obtained Ara h 2 had a purity of 94.44%. Antibody characterization revealed that the IgE-EsAbs recognized the target IgE epitope(s) of Ara h 2 and exhibited high specificity. Accordingly, an IgE-EsAbs-based sELISA using these antibodies was able to detect Ara h 2 and its allergenic residues in food samples, with high sensitivity (a limit of detection of 0.98 ng/mL), accuracy (a mean bias of 0.88%), precision (relative standard deviation < 16.50%), specificity, and recovery (an average recovery of 98.28%). Moreover, the developed sELISA could predict IgE-binding variations of Ara h 2 and peanut in foods, as verified by using sera IgE derived from peanut-allergic individuals.ConclusionThis novel immunoassay could be a user-friendly method to monitor low level of Ara h 2 and to preliminary predict in vitro potential allergenicity of Ara h 2 and peanut in processed foods.

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Section: Resultsmentioning

confidence: 99%

A novel IgE epitope-specific antibodies-based sandwich ELISA for sensitive measurement of immunoreactivity changes of peanut allergen Ara h 2 in processed foods

Yan,

Li,

Long

et al. 2024

Front. Nutr.

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“…These results provide further support for the substantial impact of mast cells on Modic changes and emphasize their potential as therapeutic targets for clinical interventions. Compound 48/80 is a strong stimulator of mast cell activation and degranulation, whereas cromolyn functions as an inhibitor 50 – 52 , suppressing mast cell release of mediators. The intraperitoneal administration of these two compounds to WT mice yielded noteworthy findings.…”

Section: Discussionmentioning

confidence: 99%

Mrgprb2-mediated mast cell activation exacerbates Modic changes by regulating immune niches

Ji,

Li,

Tao

et al. 2024

Exp Mol Med

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Modic changes are radiographic features associated with microfracture, low-virulence organism infection and chronic inflammation with inflammatory cell infiltration in the vertebral endplate region. Mast cells, as innate immune cells similar to macrophages, are present in painful degenerated intervertebral discs. However, the involvement and mechanisms of mast cells in the development of Modic changes remain unclear. Herein, we found increased mast cell infiltration in samples from patients with Modic changes and in mouse models of Modic changes. To clarify the role of mast cells in the progression of Modic changes, we used mast cell-deficient (KITW-SH/W-SH) mice to construct a model of Modic changes and found that the severity of Modic changes in KITW-SH/W-SH mice was significantly lower than that in WT mice. These findings were further supported by the use of a mast cell-specific activator (compound 48/80) and a stabilizer (cromolyn). Furthermore, we found that mast cells were not activated via the classic IgE pathway in the Modic change models and that Mrgprb2 is the specific receptor for mast cell activation reported in recent studies. Then, we utilized Mrgprb2 knockout mice to demonstrate that Mrgprb2 knockout inhibited mast cell activation and thus reduced the degree of Modic changes. Transcriptomic sequencing revealed aberrant PI3K-AKT and MAPK pathway activation in the Mrgprb2-deficient mast cells. Additionally, Mrgpbrb2-activated mast cells regulate immune niches by recruiting macrophages, promoting M1 polarization and reducing M2 polarization, thereby promoting the progression of Modic changes. These findings suggest that mast cells may serve as a novel therapeutic target for addressing Modic changes.

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“…The advancement of biosensors that allow allergen detection and evaluation of allergy drugs was also studied. Jeong et al [ 153 ] constructed a bioelectronic sensor based on nanovesicles combined with anti-immunoglobulin E (anti-IgE) antibody receptors for signal amplification. The sensing system showed that it was sensitive and selectively able to detect the peanut allergen Arachis hypogaea 2 (Ara h 2) with a LOD of 0.1 fM in real food samples such as peanut and egg white.…”

Section: Biosensors In Food Safety and Securitymentioning

confidence: 99%

Biosensor in smart food traceability system for food safety and security

Meliana,

Liu,

Show

et al. 2024

Bioengineered

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The burden of food contamination and food wastage has significantly contributed to the increased prevalence of foodborne disease and food insecurity all over the world. Due to this, there is an urgent need to develop a smarter food traceability system. Recent advancements in biosensors that are easy-to-use, rapid yet selective, sensitive, and cost-effective have shown great promise to meet the critical demand for onsite and immediate diagnosis and treatment of food safety and quality control (i.e. point-of-care technology). This review article focuses on the recent development of different biosensors for food safety and quality monitoring. In general, the application of biosensors in agriculture (i.e. pre-harvest stage) for early detection and routine control of plant infections or stress is discussed. Afterward, a more detailed advancement of biosensors in the past five years within the food supply chain (i.e. post-harvest stage) to detect different types of food contaminants and smart food packaging is highlighted. A section that discusses perspectives for the development of biosensors in the future is also mentioned.

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Adaptive biosensing platform using immune cell-based nanovesicles for food allergen detection

Cited by 7 publications

References 38 publications

A novel IgE epitope-specific antibodies-based sandwich ELISA for sensitive measurement of immunoreactivity changes of peanut allergen Ara h 2 in processed foods

A novel IgE epitope-specific antibodies-based sandwich ELISA for sensitive measurement of immunoreactivity changes of peanut allergen Ara h 2 in processed foods

Mrgprb2-mediated mast cell activation exacerbates Modic changes by regulating immune niches

Biosensor in smart food traceability system for food safety and security

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