Adaptive response of reproduction to high-altitude hypoxic stress by altering mRNA expression of hypoxia-inducible factors in female yaks (<i>Bos grunniens</i>)

He, Xiang-Dong; Xia, Yi; Jige, Mo-Ti; Wang, Hui; Zi, Xiang-Dong

doi:10.1080/10495398.2019.1588128

Cited by 4 publications

(6 citation statements)

References 22 publications

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“…In addition, Gillies et al have also confirmed that mucosal regions of the rat oviduct frequently experience severe hypoxia and that there are compartment-specific variations in the expression of endogenous hypoxia-related antigens, including the HIF isoforms (Gillies, Robinson, McPhail, Carter, & Murray, 2011). Most recently, He et al (2020) found that higher mRNA levels of HIF-1α and HIF-2α occur in the oviduct and anterior pituitary compared with the hypothalamus, ovary and uterus in yak, and both of these are higher in these organs of yak than in cattle. These data provide evidence that adaptation of reproduction to hypoxic conditions is associated with the expression of HIF-1α and HIF-2α in the reproductive axis of the female yak (He et al, 2020).…”

Section: Discussionmentioning

confidence: 92%

“…It is clear that the mechanism of HIF-1α participation in hypoxic regulation is very complex. Most recently, He et al (2020) found that HIF-1α mRNA is expressed in the hypothalamus, anterior pituitary, oviduct, ovary and uterus, but the variation in HIF-1α expression throughout the reproductive cycle was not analysed in their experiment (He et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Most recently, He et al. (2020) found that higher mRNA levels of HIF‐1α and HIF‐2α occur in the oviduct and anterior pituitary compared with the hypothalamus, ovary and uterus in yak, and both of these are higher in these organs of yak than in cattle. These data provide evidence that adaptation of reproduction to hypoxic conditions is associated with the expression of HIF‐1α and HIF‐2α in the reproductive axis of the female yak (He et al., 2020).…”

Section: Discussionmentioning

confidence: 99%

“…A recent study found that HIF-1α mRNA is expressed in the hypothalamus, anterior pituitary, oviduct, ovary and uterus of the yak. These data provide evidence that adaptation of reproduction to hypoxic conditions is associated with the expression of HIF-1α in the reproductive axis of the female yak (He, Xia, Jige, Wang, & Zi, 2020). Therefore, it is important to investigate the roles of HIF-1α in reproduction in order to understand the mechanism of hypoxic adaptation and improve the reproductive efficiency of the yak.…”

Section: Introductionmentioning

confidence: 90%

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The expression of hypoxia‐inducible factor‐1 alpha in primary reproductive organs of the female yak (Bos grunniens) at different reproductive stages

Fan

Han

et al. 2020

Reprod Domestic Animals

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The yak (Bos grunniens) is the most important livestock animal in high-altitude regions owing to its prominent adaptability to cold conditions, nutritional deficiencies and hypoxia. The reproductive organs exhibit different histological appearances and physiological processes at different reproductive stages. Hypoxia-inducible factor-1 alpha (HIF-1α) is the regulatory subunit of HIF-1 that crucially regulates the response to hypoxia in mammalian organisms. The goal of our study was to investigate the expression and distribution of HIF-1α in the primary yak reproductive organs at different reproductive stages. Samples of the ovary, oviduct and uterus of 15 adult female yaks were collected and used in the experiment. The expression and localization of HIF-1α proteins and mRNA were investigated using quantitative real-time polymerase chain reaction (qRT-PCR), Western blot (WB) and immunohistochemistry (IHC). The results indicated that the expression of HIF-1α protein in the ovary was higher during the luteal phase than during the follicular phase and gestation period (p < .05). In the oviduct, HIF-1α protein was also more highly expressed during the luteal phase than during the follicular phase and gestation period (p < .01). However, in the uterus, the HIF-1α protein had stronger expression during the gestation period than during the follicular phase (p < .01) and luteal phase (p < .05). The expression of HIF-1α mRNA was similar to that of its protein. Immunohistochemical analysis revealed intense immunostaining of HIF-1α proteins in the follicular granulosa cells, granular luteal cells, villous epithelial cells of the oviduct, endometrial glandular epithelium and luminal epithelium, foetal villous trophoblast, and epithelia of caruncular crypts. This study showed that the expression of HIF-1α in the ovary, oviduct and uterus varies according to the stage of the reproductive cycle. This implies that HIF-1α plays an important role in regulating the stage-specific physiological function of yak reproductive organs under hypoxic environments.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 90%

See 2 more Smart Citations

The expression of hypoxia‐inducible factor‐1 alpha in primary reproductive organs of the female yak (Bos grunniens) at different reproductive stages

Fan

Han

et al. 2020

Reprod Domestic Animals

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“…In rats, hypoxia also impacts reproduction and fertility [ 51 , 52 ]. He et al describe that the adaptation of reproduction in hypoxic conditions is associated with a greater expression of HIF-1α in the reproductive axis of female yaks [ 53 ]. The HIF-1 expression in oviductal tissue increased in patients with uterine myoma, reflecting accession to the pathology of the uterus disorders in the oviduct.…”

Section: Discussionmentioning

confidence: 99%

Oviductal Oxygen Homeostasis in Patients with Uterine Myoma: Correlation between Hypoxia and Telocytes

Wrona¹,

Aleksandrovych

Bereza

et al. 2022

IJMS

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Oxygen balance is crucial for angiogenesis, immunity, and tissue repair. The human oviduct is essential for reproductive function, and any imbalance in homeostasis leads to fertility disturbances and might be a reason for ectopic pregnancy development. Uterine myoma is a widespread benign tumour, which is often accompanied by infertility. Telocytes have been discussed in the contexts of motility, fibrosis development, and angiogenesis. We observed the oviducts from patients with and without uterine myoma, comparing the expression of HIF-1, HO, VEGF and its receptor, NOS, oestrogen, and progesterone receptors by immunolabeling. The myometrial and oviductal telocytes were also compared in both groups. Biochemical analyses were conducted for FSH, LH, AMH, sFlt, oestrogen, and progesterone in blood samples. Patients with uterine myoma have different expressions of sex steroid receptors and an increased number of telocytes. The decreasing VEFG expression was compensated by the rise in the HIF-1 and NOS expression. Blood biochemical analyses revealed a higher progesterone level and lower AMH in patients with uterine myoma. No differences in sFlt, FSH, and LF were observed. Uterine myoma impacts oviduct oxygen homeostasis and might cause fertility disturbances (uterine and oviductal infertility factors).

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Single‐cell transcriptomic survey of cell diversity and functional changes in yak hearts at different altitude

et al. 2023

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The yak (Bos grunniens) is a species adapted to the hypoxic environment in the plateau area. The heart is a hypoxia-sensitive organ involved in this adaptation. Herein, we used single-cell RNA-seq technology and clustering to determine the presence of 11 cell populations in the yak heart. We analyzed gene expression differences and expression patterns in each cell subpopulation at different altitudes. The cells related to altitude changes are mainly smooth muscle cells and vascular endothelial cells. Of the four transcription factors (TFs, MEF2B, FOXP4, ARID5A, and HES4) found in smooth muscle cells, only MEF2B was specifically expressed in vascular smooth muscle cells. Three key TFs (HNF1B, DMRTA1, and ARNTL2) were also found in the cardiomyocyte module. Compared with data extracted from low-altitude yak, we observed that the high altitude yak has enhanced contraction and relaxation of vascular smooth muscle cells and an increased metabolic level of cardiomyocytes. These may be strategies for the yak to adapt to high-altitude hypoxia environments.

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Adaptive response of reproduction to high-altitude hypoxic stress by altering mRNA expression of hypoxia-inducible factors in female yaks (Bos grunniens)

Cited by 4 publications

References 22 publications

The expression of hypoxia‐inducible factor‐1 alpha in primary reproductive organs of the female yak (Bos grunniens) at different reproductive stages

The expression of hypoxia‐inducible factor‐1 alpha in primary reproductive organs of the female yak (Bos grunniens) at different reproductive stages

Oviductal Oxygen Homeostasis in Patients with Uterine Myoma: Correlation between Hypoxia and Telocytes

Single‐cell transcriptomic survey of cell diversity and functional changes in yak hearts at different altitude

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