Adaptive Role of Glial Cell Line-Derived Neurotrophic Factor in Cerebral Ischemia

Митрошина, Е.В.; Abogessimengane, B.Zh.; Urazov, M.D.; Hamraoui, I.; Mishchenko, Tatiana A.; Astrakhanova, T.A.; Щелчкова, Н А; Lapshin, R.D.; Shishkina, T. V.; Belousova, І. A.; Мухина, И.В.; Vedunova, Maria

doi:10.17691/stm2017.9.1.08

Cited by 4 publications

(1 citation statement)

References 14 publications

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“…В настоящее время исследователи не пришли к единому выводу о диагностической и прогностической значимости определения уровня GDNF у больных инсультом, что требу ет дальнейшего изучения вопроса [3][4][5][6].…”

Section: Introductionunclassified

Prognostic value of assessment of glial cell line-derived neurotrophic factor in ischemic stroke patients

et al. 2021

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Objective. The aim of the study was to estimate the prognostic value of GDNF in acute IS patients. Glial neurotrophic factor (GDNF) is a neuropeptide, which promotes the proliferation and differentiation of the cells of the central and peripheral nervous system, possesses antihypoxic and neuroprotective properties in ischemic stroke (IS). Materials and methods. 50 patients with first-ever acute IS were examined at the National Institutes of Health Stroke Scale (NIHSS); modified Rankin scale was used on admission to the hospital, 7 and 14 days after stroke. The plasma GDNF level was measured at the first 48 hours and after 7 days of IS onset. GDNF was analyzed by commercially available ELISA kit. The comparison group included 20 healthy persons. Results. The average GDNF level in the group of patients with IS (3.4 [2.8; 5.0] pg/ml) was significantly higher than in the group of healthy individuals (2.8 [2.6; 2.9] pg/ml), p = 0.02. The risk of unfavourable functional outcome on the day 14 after IS onset was higher by 2.8-fold in those patients whose level of GDNF was increased on the 7th day of the disease by more than 30% compared to its level in the first 48 hours (RR = 2,8; 95% CI [1,45,7]). The GDNF level less than 2.9 pg/ml was associated with a high risk of death during 14 days after the IS onset (RR = 6,7; 95% CI [1,530,5]). Conclusions. Plasma concentrations of GDNF have a high predictive value for unfavorable functional outcome and risk of death in acute period of IS.

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Section: Introductionunclassified

Prognostic value of assessment of glial cell line-derived neurotrophic factor in ischemic stroke patients

et al. 2021

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Common Carotid Artery Occlusion and Double-Nucleated Cellular Structures In The Rat Sensorimotor Cerebral Cortex

Авдеев

Akulinin

Stepanov

et al. 2021

Obŝaâ reanimatologiâ

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The aim of the study. To study the double-nucleated cellular structures of the brain sensorimotor cortex (SMC) of sexually mature white rats after a 40-minute occlusion of the common carotid arteries.Methods. Acute ischemia was simulated in white Wistar rats by 40-minute occlusion of the common carotid arteries (OCCA). We performed comparative morphometric evaluation of cyto-, dendro-, synapto-, and glioar-chitectonics of the neocortex in intact animals (n=5), and 1 (n=5), 3 (n=5), and 7 days (n=5) after OCCA. We used Nissl, hematoxylin and eosin staining, and immunohistochemical reactions for NSE, MAP-2, HSP-70, p38, caspase-3, GFAP, AIF1, and Ki-67. Numerical density of pyramidal neurons, oligodendrocytes (ODCs), mi-croglyocytes (MGCs), presence of dystrophic and necrobiotic neurons with one or more nucleoli, hetero- and dikaryons were assessed. Statistical hypotheses were tested using Statistica 8.0 software.Results. The percentage of dystrophic and necrobiotic neurons, nerve cells with two nuclei or two or more nucleoli, the total number (proliferation) and percentage of hypertrophic astrocytes, ODCs and MGCs increased significantly after OCCA. The total numerical density of SMC neurons decreased by 26.4% (P=0.001) in layer III and by 18.5% in layer V (Mann-Whitney U Test; P=0.01) after OCCA throughout the observation period. Pathological and compensatory changes were diffusely focal and more pronounced in layer III of the neocortex. The density of bi-nucleated heterokaryons and dikaryons remained unchanged on days 1 and 3 after OCCA vs control and was 3.5 (1.5-4.0)/mm2, and increased to 6.5 (5.0-8.5)/mm2 on day 7 (Mann-Whitney U Test; P=0.002). This increase occurred along with a higher density of ODCs and MGCs than in the control. The maximum number of neurons with two or more nucleoli was also noted in layer III and V during this period.Conclusion. After 40-minute OCCA in SMC, parallel to the dystrophic and necrobiotic changes of pyramidal neurons and activation of neuroglial cells, there was an increase in the formation of heterokaryons and neurons with amplified nucleolus. These changes were considered as a variant of neuronal response to ischemic damage.

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Biochemical factors of hypoxia and their role in assessing the functional state of the fetus

Rozhdestvenskaya

Kokaya

Bezhenar

2021

Journal of obstetrics and women's diseases

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The constant frequent incidents of fetal hypoxia during pregnancy and childbirth remain the leading unsolved problem in modern practical obstetrics. In some cases, the onset of a pathological process can be diagnosed earlier due to the on-time monitoring of functional disorders of the fetus. However, the existing diagnostic methods do not show the compensatory and adaptive capabilities of the fetus; do not lead to an in-depth understanding of the pathophysiology of this condition and do not contribute to the implementation of evidence-based therapy. This review summarizes current knowledge about the diagnosis of functional disorders of the fetus and discusses possible ways of assessing adaptive mechanisms in response to stress during pregnancy and childbirth. The article shows the development of biochemical methods for diagnosing functional disorders of the fetus. The putative biochemical markers for assessing the compensatory capabilities of the fetus during pregnancy and childbirth are presented.

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Adaptive Role of Glial Cell Line-Derived Neurotrophic Factor in Cerebral Ischemia

Cited by 4 publications

References 14 publications

Prognostic value of assessment of glial cell line-derived neurotrophic factor in ischemic stroke patients

Prognostic value of assessment of glial cell line-derived neurotrophic factor in ischemic stroke patients

Common Carotid Artery Occlusion and Double-Nucleated Cellular Structures In The Rat Sensorimotor Cerebral Cortex

Biochemical factors of hypoxia and their role in assessing the functional state of the fetus

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