Adding value to secondary streams of corn wet milling industry

Abstract

Corn steep liquor (CSL) is spontaneously fermented, mainly by thermophilic Bacillus strains, producing lipopeptide biosurfactants. These biosurfactants can be extracted with ethyl acetate at the same time that an aqueous phase is generated (named CSL-plus). The results showed that the biosurfactant extraction produced a liquor with a reduced content of fats and minor changes in the micronutrients concentration. The decrease in the fatty acid content is due to the extraction of biosurfactants and free fatty acids contained in the biosurfactant extract, composed mainly of C16 and C18 fatty acids. Therefore, it can be concluded that the extraction of biosurfactants gives a nutritional supplement (CSL-plus) with lower content of fats, improving the nutritional properties of CSL and consequently added value is given to the water stream generated. Moreover, it was demonstrated that the biosurfactant extract from CSL could be used to improve the solubilization of chicken broth cubes.

Optimization of the Operational Conditions to Produce Extracellular and Cell-Bound Biosurfactants by Aneurinibacillus aneurinilyticus Using Corn Steep Liquor as a Unique Source of Nutrients

“…Moreover, the CSLs under evaluation were not subject to a stabilization phase where CSL was concentrated by up to 50% in solids, usually through the evaporation of water. Moreover, Martínez-Arcos et al [30] analyzed CSL from FeedStimulants (50% in solids), corroborating the presence of minerals, amino acids (in equivalents of proteins), sugars, and organic acids in consonance with the typical composition of CSL. Hence, it would be interesting to increase our knowledge of the conditions that stimulate biosurfactant production by A. aneurinilyticus in CSL.…”

“…For the assay, CSL provided by FeedStimulants (Zoetermeer, The Netherlands) was used. The composition of this CSL was evaluated in previous work [30]. The solutions (100 mL) of CSL (at 100, 250, 400 g L −1 ) were prepared in 250 mL Erlenmeyer flasks by dissolving this liquor in demineralized water, which was then sterilized at 121 • C for 15 min.…”

Combined Application of Myo-Inositol and Corn Steep Liquor from Agricultural Waste Alleviate Salt Stress in Brassica rapa

“…Agricultural wastes often contain bioactive substances, so the development of plant growth stimulants from agricultural wastes can play a positive role in promoting plant growth and resilience [ 9 ]. Corn steep liquor (CSL), a concentrated byproduct from corn starch production, is an acidic viscous liquid rich in various nutrients, including amino acids, sugars, proteins, vitamins, and minerals [ 10 ]. The diverse nutritional composition of CSL allows for versatile applications across various fields, such as additives in biotechnological production media, animal feed, surfactant production, and a component in biostimulants [ 11 ].…”