2016
DOI: 10.3390/toxins8110338
|View full text |Cite
|
Sign up to set email alerts
|

Addition of Carbon to the Culture Medium Improves the Detection Efficiency of Aflatoxin Synthetic Fungi

Abstract: Aflatoxin (AF) is a harmful secondary metabolite that is synthesized by the Aspergillus species. Although AF detection techniques have been developed, techniques for detection of AF synthetic fungi are still required. Techniques such as plate culture methods are continually being modified for this purpose. However, plate culture methods require refinement because they suffer from several issues. In this study, activated charcoal powder (carbon) was added to a culture medium containing cyclodextrin (CD) to enha… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

1
10
0

Year Published

2018
2018
2024
2024

Publication Types

Select...
8

Relationship

2
6

Authors

Journals

citations
Cited by 11 publications
(11 citation statements)
references
References 33 publications
1
10
0
Order By: Relevance
“…By contrast, the α-CD-containing PDA showed enhanced light scattering compared with the control PDA. These results were consistent with those from a previous study [14].…”
Section: Resultssupporting
confidence: 94%
See 1 more Smart Citation
“…By contrast, the α-CD-containing PDA showed enhanced light scattering compared with the control PDA. These results were consistent with those from a previous study [14].…”
Section: Resultssupporting
confidence: 94%
“…A modified CD-addition method, which uses co-treatment with both CD and activated carbon, has been reported to improve the visibility of AF-derived fluorescence [14] (Figure 1). The improved visibility can be attributed to the fact that carbon addition reduces the reflection and scattering of UV light from the surface of the culture plate.…”
Section: Introductionmentioning
confidence: 99%
“…Additionally, since light irradiation caused changes in fungal phenotypes regardless of the strain, Aspergillus bombysis MAFF111712 and Aspergillus nomius MAFF111739 were added to this test. The observational test of AF-derived fluorescence was conducted using PDA containing α-cyclodextrin (α-CD) and activated carbon to increase the visibility of the fluorescence [ 20 , 21 , 22 ]. AF-derived fluorescence was observed from the undersides of the culture plates.…”
Section: Resultsmentioning
confidence: 99%
“…Consequently, the nutrient level of YES agar medium is much sufficient for A. terreus than that in MEA. Thus, many researchers stated YES to be the best media for hyphal growth (Maheswari, Singh, & Sahu, 1999;Saha et al, 2008;Suzuki & Iwahashi, 2016;S. Xu, Yuan, & Chen, 1984).…”
Section: Resultsmentioning
confidence: 99%