Addition of hydrogen peroxide to methylene blue conjugated to β-cyclodextrin in photodynamic antimicrobial chemotherapy in S. mutans biofilm

Sales, Luciana Solera; Guimarães, Gustavo Narvaes; Wijesinghe, Gayan Kanchana; Moreira, Kelly Maria Silva; Jóia, Felipe; Stipp, Rafael Nóbrega; Rodrigues, Lidiany Karla Azevedo; Nobre‐dos‐Santos, Marinês; Steiner‐Oliveira, Carolina

doi:10.1016/j.pdpdt.2019.09.004

Cited by 9 publications

(3 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…High-level laser therapy is based on the combination of a penetrating laser with a stabilized HP [20]. Multiple studies have concluded improved antimicrobial efficacy when hydrogen peroxide (HP) was employed in PDI [19,[21][22][23]. Sales, et al, stated that HP has provided similar characteristics as a photosensitizer [23].…”

Section: Introductionmentioning

confidence: 99%

“…Multiple studies have concluded improved antimicrobial efficacy when hydrogen peroxide (HP) was employed in PDI [19,[21][22][23]. Sales, et al, stated that HP has provided similar characteristics as a photosensitizer [23]. The combination of a highfrequency diode laser (980 nm) and HP, if compared with classic PDI accomplished with photosensitizers, allows deeper penetration into the biofilm [24].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synergistic Antimicrobial Effect of Photodynamic Inactivation and SWEEPS in Combined Treatment against Enterococcus faecalis in a Root Canal Biofilm Model: An In Vitro Study

2023

View full text Add to dashboard Cite

Background: Persistent bacterial infections caused by biofilm-associated Enterococcus faecalis are a global public health concern. This study aims to compare the biofilm removal effects of methylene blue (MB) or hydrogen peroxide (HP) mediated photodynamic inactivation (PDI) with a shock wave enhanced emission photoacoustic streaming (SWEEPS) method laser irradiation enhanced bactericidal activity. Methods: A total of fifty extracted single-rooted human teeth were used. Each canal was then inoculated with a bacterial suspension of E. faecalis and left for ten days to induce biofilm formation. The antibacterial effects within treated root canals using MB or HP + diode laser or SWEEPS alone or in combination were assessed and compared with 5.25% sodium hypochlorite (NaOCl) as the positive control group. Data were analyzed using one-way ANOVA and Tukey’s post hoc test. Results: The MB + diode laser + SWEEPS and HP + diode laser + SWEEPS groups displayed significantly lower amounts of bacteria than either the MB + diode laser or SWEEPS and HP + diode laser or SWEEPS groups (p < 0.001). There was no statistically significant difference detected between the diode laser and SWEEPS groups (p = 0.982). Conclusions: SWEEPS can enhance the photosensitizer distribution in the root canal, leading to effective biofilm removal. This technique could thus help promote the future development of endodontic treatments.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synergistic Antimicrobial Effect of Photodynamic Inactivation and SWEEPS in Combined Treatment against Enterococcus faecalis in a Root Canal Biofilm Model: An In Vitro Study

2023

View full text Add to dashboard Cite

show abstract

“…The FDA has approved using HP at less than 3% as an oral debriding agent. It has been shown that the addition of 0.3% HP enhanced the antimicrobial effects of MB-PDT [ 34 , 35 , 36 ], although another study [ 37 ] did not find any enhancement at 40 µM (0.12%) HP. Our data showed that the additional 0.1% HP could enhance the killing efficacy of MB-PDT under anaerobic conditions, but it could not restore its efficacy to the level when the treatment was performed with air.…”

Section: Discussionmentioning

confidence: 99%

Oxygen Availability on the Application of Antimicrobial Photodynamic Therapy against Multi-Species Biofilms

et al. 2023

View full text Add to dashboard Cite

Methylene blue (MB)- or Curcumin (Cur)-based photodynamic therapy (PDT) has been used as an adjunctive treatment for periodontitis. Its actual clinical efficacy is still in question because the lack of oxygen in a deep periodontal pocket might reduce the PDT efficacy. We aim to investigate the effect of oxygen on PDT efficacy and to examine if the addition of hydrogen peroxide (HP) could improve PDT performance anaerobically. To this end, we cultured 48 h saliva-derived multi-species biofilms and treated the biofilms with 25 µM MB or 40 µM Cur, HP (0.001%, 0.01% and 0.1%), light (L-450 nm or L-660 nm), or combinations thereof under ambient air or strictly anaerobic conditions. MB- and Cur-PDTs significantly reduced biofilm viability in air but not under anaerobic conditions. HP at 0.1% significantly enhanced the killing efficacies of both MB- and Cur-PDTs anaerobically. The killing efficacy of Cur-PDT combined with 0.1% HP was higher anaerobically than in air. However, this was not the case for MB-PDT combined with 0.1% HP. In conclusion, this study demonstrated that the biofilm killing efficacies of MB- and Cur-PDTs diminished when there was no oxygen. HP at 0.1% can enhance the efficacy of PDT performed anaerobically, but the level of enhancement is photosensitizer-dependent.

show abstract

Inhibition of S. mutans after nanoparticle mediated photodynamic antimicrobial chemotherapy on oral biofilm flow-cell system using laser or LED

et al. 2021

View full text Add to dashboard Cite

Addition of hydrogen peroxide to methylene blue conjugated to β-cyclodextrin in photodynamic antimicrobial chemotherapy in S. mutans biofilm

Cited by 9 publications

References 48 publications

Synergistic Antimicrobial Effect of Photodynamic Inactivation and SWEEPS in Combined Treatment against Enterococcus faecalis in a Root Canal Biofilm Model: An In Vitro Study

Synergistic Antimicrobial Effect of Photodynamic Inactivation and SWEEPS in Combined Treatment against Enterococcus faecalis in a Root Canal Biofilm Model: An In Vitro Study

Oxygen Availability on the Application of Antimicrobial Photodynamic Therapy against Multi-Species Biofilms

Inhibition of S. mutans after nanoparticle mediated photodynamic antimicrobial chemotherapy on oral biofilm flow-cell system using laser or LED

Contact Info

Product

Resources

About