Addition of Pentoxifylline to Skim Milk–Based Extender on Frozen-Thawed Equine Sperm

Tsunoda, Roberta Harue; Arruda, Rubens Paes de; Serrano-Recalde, Elena Carolina; Oliveira, Bruna Marcele Martins de; Florez-Rodriguez, S. A.; Alves, Maíra Bianchi Rodrigues; Nichi, Marcílio; Celeghini, Eneiva Carla Carvalho

doi:10.1016/j.jevs.2015.08.001

Cited by 5 publications

(8 citation statements)

References 22 publications

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“…Although PTX had a positive effect on TM and PM, it did not improve VCL. This result conflicts with other research on equine epididymal (Guasti et al 2013) and ejaculated (Gradil and Ball 2000;Tsunoda et al 2015) spermatozoa. It may have been caused by leaving epididymal plasma and its immobilization factors with the spermatozoa during cryopreservation (the samples were not centrifuged after flushing the epididymis).…”

Section: Discussioncontrasting

confidence: 99%

“…Guasti et al (2013) found that adding PTX had a positive effect on motility of stallion epididymal spermatozoa. One study found no increase of motility in ejaculated spermatozoa after adding PTX (Tsunoda et al 2015). The dosage of PTX in the studies mentioned above was around 3.6 mM and 7.18 mM, which is equal to a concentration of 1 mg/ml and 2 mg/ml, respectively.…”

Section: Discussionmentioning

confidence: 94%

“…Pentoxifylline (PTX) and caffeine (CAF) are readily available and their effect on ejaculated sperm motility has been studied before (Gradil and Ball 2000;Melo-Oña et al 2012;Stephens et al 2013;Tsunoda et al 2015). However, information about their effect on epididymal spermatozoa is scarce (Guasti et al 2013).…”

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confidence: 99%

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Influence of pentoxifylline and caffeine on stallion epididymal sperm motility after thawing

et al. 2021

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Two substances at two concentrations each were added to frozen-thawed stallion epididymal spermatozoa to improve motility indicators, which were assessed 30, 60, 120, 180 and 240 min after thawing. Pentoxifylline was added at concentrations of 3.6 mM (1 mg/ml) and 7.18 mM (2 mg/ml). Both concentrations had a positive effect on total and progressive motility of spermatozoa throughout the study. The other substance, caffeine, was added at concentrations of 2 mM (0.4 mg/ml) and 5.5 mM (1 mg/ml). Both concentrations had a positive effect on total and progressive motility of spermatozoa only 30 and 60 min after thawing. Subsequently, 180 min after thawing, improvement was found only in total sperm motility.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 94%

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Influence of pentoxifylline and caffeine on stallion epididymal sperm motility after thawing

et al. 2021

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“…Therefore, the main goal of this study was to investigate the PTX ability on post-thaw quality of goat spermatozoa compared to BME, which is a widely used antioxidant in freezing diluents. It is generally accepted that PTX exerts a protecting influence on assessed cryopreserved parameters against the detrimental effects of cryopreservation process (Bell et al, 1993;Esteves et al, 1998Esteves et al, , 2007Tsunoda et al, 2015), which demonstrate the findings in the current experiment.…”

Section: Ta B L E 2 Effects Of Pentoxifylline (Ptx) and Basal Mediumsupporting

confidence: 80%

Evaluation of pentoxifylline and Basal Medium Eagle supplemented to diluent on cryopreserved goat spermatozoa

Nazari

Farshad

Vaziry

et al. 2020

Reprod Domestic Animals

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Cryopreservation process causes extensive damage to the mammalian spermatozoa (Maxwell & Johnson, 1999; Purdy, 2006). Over the past decade, experiments indicated that freezing and thawing process are associated with oxidative stress and generation of reactive oxygen species (ROS) and finally lipid peroxidation. Moreover, the alteration in the acrosome and membrane integrity, decrease in viability, motility and fertilizing capability are the consequences of freezing and thawing processes (Chatterjee, De Lamirande, &

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“…As a result, semen quality and fertility of these animals are quite variable and some stallions may have poor quality sperm (Allen and Wilsher, 2011), which leads to great difficulty of large-scale use of equine cryopreserved semen (Al-Essawe et al, 2018). Hence, many studies have been performed to improve stallion semen quality in order to increase the efficiency of reproductive biotechniques (Affonso et al, 2017;Lançoni et al, 2018;Nascimento et al, 2008;Tsunoda et al, 2015). Peña et al (2015) demonstrated that mitochondria are key structures in sperm function and may undergo major changes during cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

Coenzyme Q-10 improves preservation of mitochondrial functionality and actin structure of cryopreserved stallion sperm

Lançoni

Celeghini

Giuli³

et al. 2021

Anim. Reprod.

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Coenzyme Q-10 (CoQ-10) is a cofactor for mitochondrial electron transport chain and may be an alternative to improve sperm quality of cryopreserved equine semen. This work aimed to improve stallion semen quality after freezing by adding CoQ-10 to the cryopreservation protocol. Seven saddle stallions were utilized. Each animal was submitted to five semen collections and freezing procedures. For cryopreservation, each ejaculate was divided in three treatments: 1) Botucrio® diluent (control); 2) 50 μmol CoQ-10 added to Botucrio® diluent; 3) 1 mmol CoQ-10 added to Botucrio® diluent. Semen batches were analyzed for sperm motility characteristics (CASA), plasma and acrosomal membranes integrity and mitochondrial membrane potential (by fluorescence probes propidium iodide, Hoechst 33342, FITC-PSA and JC-1, respectively), alterations in cytoskeletal actin (phalloidin-FITC) and mitochondrial function (diaminobenzidine; DAB). The 1 mmol CoQ-10 treatment presented higher (P<0.05) amount (66.8%) of sperm cells with fully stained midpiece (indicating high mitochondrial activity) and higher (P<0.05) amount (81.6%) of cells without actin reorganization to the post-acrosomal region compared to control group (60.8% and 76.0%, respectively). It was concluded that the addition of 1 mmol CoQ-10 to the freezing diluent was more effective in preserving mitochondria functionality and cytoskeleton of sperm cells submitted to cryopreservation process.

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Addition of Pentoxifylline to Skim Milk–Based Extender on Frozen-Thawed Equine Sperm

Cited by 5 publications

References 22 publications

Influence of pentoxifylline and caffeine on stallion epididymal sperm motility after thawing

Influence of pentoxifylline and caffeine on stallion epididymal sperm motility after thawing

Evaluation of pentoxifylline and Basal Medium Eagle supplemented to diluent on cryopreserved goat spermatozoa

Coenzyme Q-10 improves preservation of mitochondrial functionality and actin structure of cryopreserved stallion sperm

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