Addition of Tomato Juice as Additive in Diluent of Egg Yolk Citrate on the Quality of Pesisir Cattle Semen

Afriani, Tinda; Jaswandi, Jaswandi; Rastosari, Adisti; Razzak, Muhammad Cadilac Al; Wahyudi, Dwiki

doi:10.17582/journal.jahp/2023/11.1.62.67

Cited by 1 publication

(3 citation statements)

References 13 publications

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“…A Neubauer chambered slide was used for counting sperm at a 1:20 dilution ( 18 ),. We then measured the average sperm density in millions/millimeter by counting them in the 4 large corners and the center squares.…”

Section: Methodsmentioning

confidence: 99%

“…Spermatozoa of 56-day-old mice were collected according to our laboratory methods (16). We carefully collected each cauda from all mice and following washing with normal saline at 37°C, they were transferred to 1.5-ml Eppendorf tubes containing 500 µl of artificial human tubular fluid (HTF, 37°C) medium (17).…”

Section: Collection Of Samplesmentioning

confidence: 99%

“…Briefly, for the sperm motility assessment, we prepared a 10-µl sample to perform computer-assisted sperm analysis (CASA), and for every single evaluation, we analyzed 30 frames in 0.5 sec and made 6 measurements, with a total of at least 2000 spermatozoa for each animal (16).…”

Section: Sperm Motilitymentioning

confidence: 99%

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Effects of hyperthyroidism and diabetes mellitus on spermatogenesis in peri- and post-pubertal mice

Shi,

Korejo,

Kamboh

et al. 2023

Front. Endocrinol.

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IntroductionDiabetes and thyroid dysfunction often co-exist. One autoimmune disorder always invites another and it has been reported that such co-morbid ailments always become detrimental to the health of the patients.Materials and methodsIn our previous work, we elucidated the interactions of diabetes and hypothyroidism on testicular development and spermatogenesis. However, the present study illuminates the interface between diabetes and hyperthyroidism, where 16 ICR pregnant primiparous mice were used and subsequently 48 male pups were randomly selected (n=12/group) and separated into 4 groups: control (C), diabetic (D), diabetic + hyperthyroidism (DH) and hyperthyroidism (H).ResultsComputerized sperm analyses showed significant reductions in count by 20% and increases of 15% in D and H animals, respectively, vs. controls. However, rapid progressive sperm motility was significantly lower only in D (30%) compared with C mice. Our histomorphometric investigation depicted damaging effects on testicular and epididymal tissues; the stroma adjacent to the seminiferous tubules of the D mice revealed edematous fluid and unstructured material. However, in the epididymis, germ cell diminution contraction of tubules, compacted principal and clear cells, lipid vacuolization, atypical cellular connections, exfoliated epithelial cells, and round spermatids were conspicuous in DH mice.DiscussionCollectively, our experiment was undertaken to ultimately better recognize male reproductive disorders in diabetic-hyperthyroid patients.

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Section: Methodsmentioning

confidence: 99%

Section: Collection Of Samplesmentioning

confidence: 99%