Objective: The aim of this research was to isolate and identify lactic acid bacteria using 16S rRNA and evaluates their potential as probiotics. Methods:The probiotic properties measured were resistance to low pH and to 0.3% and 0.5% bile salts, antimicrobial activity against pathogenic bacteria (Escherichia coli O157:H7 and Staphylococcus aureus ATCC 25923), antibiotic resistance, and hydrophobicity. Results:The lactic acid bacteria with optimal probiotic properties were isolated from buffalo milk and identified from a sample from Agam district (BMA 3.3) which was classified using BLAST analysis as a strain of Lactobacillus fermentum (L23). Conclusion:Buffalo milk from this part of West Sumatera contains a strain of L. fermentum with has good probiotic properties.

The purpose of this study was to know the quality of Boer liquid semen during storage by adding sweet orange essential oil and gentamicin into the tris yolkextender.The semenlongevity test was carried out by storing semen in a closed tube at room temperature and refrigerator, and evaluated motility and Viability every 3 hours at room temperature and 12 hours in refrigerator. The results showed that the characteristics of the liquid semen, with the addition of 1% of essential oils showed percentage of motility and Viability significantly higher than 0.5% and without the addition of essential oil, either stored at room temperature or in the refrigerator. This may be related to the content of essential oils of sweet orange peel containing flavonoids and antibacterials that are capable of maintaining liquid semen quality of Boer Goat.In addition, gentamicin contains antibacterials capable of suppressing the growth of bacteria which can damage and durabilitythe spermatozoa.Keywords: Boer Goat,Gentamicin, Liquid semen, Sweet orange

bulan; masa kosong (Days Open) 260,63±147,04 hari dan 192,26±114,04 hari; sedangkan selang beranak (calving interval) 548,63±168,30 hari dan 477,26±114,04 hari. Performa reproduksi di kedua daerah ini belum baik karena melebihi jangka waktu optimal untuk ketiga variabel tersebut. Tidak ada perbedaan (P>0,05) antara penampilan reproduksi sapi perah di kedua daerah penelitian. Kata kunci : sapi perah, performans reproduksi, umur beranak pertama, masa kosong, selang beranak

Penelitian ini dilakukan untuk mengetahui kecepatan timbulnya berahi dari dosis Gonadotropin Releasing Hormone (GnRH) yang berbeda dan pengaruh waktu pemberian GnRH terhadap jumlah korpus luteum pada sapi Pesisir. Materi yang digunakan yaitu 12 ekor induk sapi Pesisir, hormon PGF2α dan GnRH. Terdiri dari dua tahap, pertama Induk sapi disinkronisasi dengan 5 ml PGF2α dan kemudian diberi GnRH 50 µg, 100 µg dan 200µg. Kedua sapi disinkronisasi dengan 5 ml PGF2α dan kemudian diberi GnRH dosis hasil penelitian tahap pertama dan dibagi atas 4 waktu penyuntikan, yaitu A(16), B(32), C(48), dan D(64) jam. Peubah yang diamati adalah waktu terjadinya estrus setelah diberi dosis yang berbeda dan jumlah korpus luteum (CL) yang terbentuk dari waktu penyuntikan yang berbeda. Pengolahan data dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan bahwa dosis GnRH yang tercepat menunjukan gejala estrus adalah 100 µg dengan rataan 45.42±2.24 jam, 200 µg rataan 51.33±4.51 jam dan 50 µg rataan 76.53±11.92 jam. Waktu pemberian GnRH berpengaruh terhadap jumlah CL yang terlihat dari jumlah CL dari masing-masing perlakuan. Perlakuan C(48) jam menunjukkan jumlah CL yang paling banyak dengan rataan 4.33±1.15 dan diikutiB(32) jam dengan rataan 3.67±1.53,perlakuan A(16) jam dengan rataan1.67±1.15perlakuan D(64) dengan rataan 1.67±0.58.

The purpose of this study was to determine the effect offertilization time and dffirent incubation systems onfertilization level by in-vitro. The mature of ovaries from indigenous cow andfresh cementfrom HolsteinFrisiancows(FH),0.9% NaClphysiological PBS,NissuiJapan, I,HEPES30pM,20 mL Heparin, 10% goat serum, 250 g/ml FSH BO medium, medium gentamisain 50 mB-O, mineral oil, alcohol, aquabidest, and I %o aceto orcein were materials and reagents. The Completely Randomized Design (CRD) in factorial pattern was used. Results shown no significant ffict on the percentage of fertitized ooq)tes and level of development of pronuclei (2PN and> 2PN) by different timing offertilization and incubation system. The development of pronucleus (IPN) showed significant (P <0.05) on I2 hours (37.60 %o), but no significant effect on dffirent incubation system. It concluded, the system of incubation and time of fertilization has no effect on oocyte fertilization rate. Oocytes fertilization time can be performed at 6 hours , I 2 hours, and I B hours, while the extension of the period of fertilization until I 8 hours did not increase the I ev el offertilization.