Additional notes on Anisopteromalus sp. (Hymenoptera: Pteromalidae), the sibling species of a parasitic wasp of stored‐product pests, Anisopteromalus calandrae (Howard): A new alternative host, an eye color mutant and DNA barcodes

Abstract

A parasitic wasp of stored‐product pests, Anisopteromalus calandrae (Howard), is known to have a sibling species with a different chromosome number. Here, we report establishment and characterization of an eye color mutant in this sibling species. The phenotype of the mutant is red eye in adults, and crossing experiments revealed that the mutant phenotype is recessive to wild type (brown eye color). We also report DNA barcode sequences (a partial sequence of mitochondrial cytochrome‐c oxidase subunit I) of A. calandrae and the sibling species to enable accurate identification of these morphologically similar species. Analyses of our laboratory strains showed that 12.6% of the analyzed sequences (82 of 652 bp) differed between the two species. Finally, we note that the seed beetle Callosobruchus chinensis (Linné) (family Bruchidae), host of our laboratory strains of the sibling species, is a new record of alternative host at the family‐level for the wasp (known hosts: Anobiidae (natural host) and Curculionidae (alternative host)).

Morphometric analysis and taxonomic revision of AnisopteromalusRuschka (Hymenoptera: Chalcidoidea: Pteromalidae) – an integrative approach

“…Later on, similar differences between some other populations presumably belonging to A. calandrae were also found (Gokhman & Timokhov, ) and their different host preferences were revealed also (Timokhov & Gokhman, ). However, the presence of the two closely related and widely distributed species with contrasting life‐history strategies in the genus Anisopteromalus got relatively little support from the expert community for a number of years (but see Quicke, ), although it is becoming increasingly accepted now (see, e.g., Sasakawa et al , , ). Of course, taxonomic ambiguity within such an important taxon needs to be solved.…”

Evaluation of parasitic potential of Anisopteromalus calandrae (Howard) against Callosobruchus maculatus (F.), Rhyzopertha dominica (F.) and Sitophilus oryzae (L.) in grains treated with diatomaceous

“…Regardless of the non-availability of methyl bromide and continuous use of aluminum phosphide as fumigant to control stored product pests (Kavallieratos et al, 2007), out of many available control tactics, only few have Protect-it been comprehensively assessed. nsect pest can be controlled by different methods like biological (Hoedjes et al, 2011), chemical (Ishii and Shimada 2012) and physical control tactics (Ghimire and Phillips 2008;Ishii 2008 Numerous kind of hymenopteran parasitoids including Lariophagus distinguendus (F), (Hymenoptera: Pteromalidae), Anisopteromalus calandrae, have been used to manage stored grain insect pests (Gokhman, 2003;Sasakawa et al, 2012). These species have a highly developed ability to detect prey and can attack numerous tested species.…”