Additional value of computer assisted semen analysis (CASA) compared to conventional motility assessments in pig artificial insemination

Broekhuijse, Marleen L.W.J.; Šoštarić, Edita; Feitsma, H.; Gadella, B.M.

doi:10.1016/j.theriogenology.2011.05.040

Cited by 70 publications

(60 citation statements)

References 53 publications

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“…When the traditional subjective visual evaluation system was used to assess sperm motility and the results were compared to fertility, a contradictory relationship was found between them (Andersson et al, 1992;Marshburn et al, 1992;Zhang et al, 1998;Januskauskas et al, 1999). Several studies demon- strated that the traditional microscopic visual technique for the determination of motile sperm ratio is inaccurate, imprecise and its repeatability is very low (Gunzel-Apel et al, 1993;Centola, 1996;Coetzee et al, 1999;Larsen et al, 2000;Broekhuijse et al, 2011). There is a lack of evidence-based information about the relationship between motility/velocity characteristics and the fertilising ability of bull spermatozoa; therefore, it is unknown whether sperm fertility could really be predicted by these parameters.…”

Section: Discussionmentioning

confidence: 99%

Correlation between bull fertility and sperm cell velocity parameters generated by computer-assisted semen analysis

Nagy

Polichronopoulos

Gáspárdy

et al. 2015

Acta Veterinaria Hungarica

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Motility is one of the most important characteristics associated with the fertilising ability of spermatozoa indicating their viability and structural integrity. Therefore, the examination of motility constitutes an integral part of semen analysis. Computer-assisted semen analysis (CASA) allows an accurate and objective assessment of different sperm motion characteristics with high repeatability. The aim of this study was to evaluate the different kinematic (velocity) parameters of frozen/thawed bull semen and determine if any of them could be correlated with their fertilising capability after insemination based on the achieved pregnancy rate. Ejaculates from 10 bulls were collected and frozen. The kinematic/velocity parameters of spermatozoa were measured by CASA and compared to the pregnancy results of almost 9,000 females artificially inseminated (AI) with frozen semen of any of the 10 tested bulls. The data of the experiments are summarised mainly with a focus on the effects of individual velocities (curvilinear velocity: VCL, straight-line velocity: VSL, average path velocity: VAP) on fertility rather than on the influence of progressive motility as a whole. We conclude that VAP is the most useful semen motility characteristic which has clinical relevance in the prediction of fertility.Key words: Semen evaluation, CASA, fertility prediction, velocity characteristics, semen motility Male fertility is an important factor in bovine reproduction, considering that the semen of any particular bull will be used for the insemination of several females. Fertility evaluation of males is primarily based on quality assessment of semen using conventional parameters such as morphology, concentration and motility of spermatozoa. The correlation between semen quality and fertility has been well documented (Bratton et al., 1956;Davis et al

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Section: Discussionmentioning

confidence: 99%

Correlation between bull fertility and sperm cell velocity parameters generated by computer-assisted semen analysis

Nagy

Polichronopoulos

Gáspárdy

et al. 2015

Acta Veterinaria Hungarica

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“…On the other hand, two more recent studies by Broekhuijse et al (2011;2012) showed that progressive motility, VCL and BCF were positively related with farrowing rate, while total motility, VAP, VSL and ALH were positively related to the total number of born piglets. Holt et al (1997) also reported that the VSL was positively related to the litter size.…”

Section: Boar Semenmentioning

confidence: 84%

Proper use and impact of ‘Computer Assisted Semen Analysis’ technique on semen evaluation of farm animals

Michos

Basioura

Boscos

et al. 2017

J Hellenic Vet Med Soc

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Objective of this review is to present the use of ‘Computer Assisted Semen Analysis’ technique application in farm animal health management, in particular the steps for accurate semen evaluation and the impact of the method in predicting male animal fertility under field conditions. Requirements for proper use of the equipment, factors affecting the evaluation process and the role of the estimated parametres for fertility under field conditions are described. Special reference is made in sperm motility evaluation. It is concluded that the method is an effective and efficient tool for semen evaluation, provided good practices are strictly applied and adhered to, by means of which valid results may be obtained.

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“…Previous studies focusing on the effects of the above-mentioned substances differ in their conclusions as well as in their methodological approaches. But the method of motility assessment may significantly influence obtained results (Broekhuijse et al 2011). We applied objective motility evaluation by means of computer-assisted sperm analysis (CASA), using the cluster analysis method, which is suitable for resolving heterogeneity of the sperm motility data in discrete subpopulations (MartinezPastor et al 2011).…”

Section: Resultsmentioning

confidence: 99%

Effects of zearalenone, α-zearalenol, and genistein on boar sperm motility in vitro

Krejcárková¹,

Folková²,

Šimoník³

et al. 2017

CZECH J ANIM SCI

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Krejcárková A., Folková P., Šimoník O., Šašková M., Krejčířová R., Drábek O., Rajmon R. (2017): Effects of zearalenone, α-zearalenol, and genistein on boar sperm motility in vitro. Czech J. Anim. Sci., 62, 435-445.Genistein (GEN) and zearalenone (ZEA), environmental oestrogens commonly present in feedstuff for pigs, are known for their effects on reproductive functions. The aim was to verify the in vitro effects of 0.5-20 µM concentrations of GEN, ZEA and its metabolite α-zearalenol (α-ZOL) on pig sperm motility. A dose-dependent increase of the immotile sperm amount against fast and medium-fast sperm clusters was observed with all three oestrogens from the lowest concentrations tested. Individual CASA (computer-assisted sperm analysis) parameters of motile sperms seemed to be less sensitive indicators. This should be considered especially in toxicological research on a sperm model. Background of inconsistencies in to date-published papers is discussed. The results shift the effective concentrations of ZEA, α-ZOL, and GEN to values achievable in vivo and raises the questions of risk assessment of these compounds in pig reproduction.

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Additional value of computer assisted semen analysis (CASA) compared to conventional motility assessments in pig artificial insemination

Cited by 70 publications

References 53 publications

Correlation between bull fertility and sperm cell velocity parameters generated by computer-assisted semen analysis

Correlation between bull fertility and sperm cell velocity parameters generated by computer-assisted semen analysis

Proper use and impact of ‘Computer Assisted Semen Analysis’ technique on semen evaluation of farm animals

Effects of zearalenone, α-zearalenol, and genistein on boar sperm motility in vitro

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