Additions and corrections: Development and characterisation of EST-SSR markers from the crown rust pathogen of ryegrass (<i>Puccinia coronata</i> Corda f.sp. <i>lolii</i>)

Dracatos, Peter M.; Dumsday, J. L.; Olle, R. S.; Cogan, Noel O. I.; Dobrowolski, Mark P.; Fujimori, Masahiro; Roderick, Hywel RoderickH.; Stewart, Alan V.; Smith, K. F.; Forster, John W.

doi:10.1139/g06-135

Cited by 8 publications

(4 citation statements)

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“…To our knowledge, simple sequence repeatexpressed sequence tag (SSR-EST) markers have only been developed to study the genetic diversity of Puccinia coronata f. sp. lolii (pathogen of ryegrass) (Dracatos et al, 2006), but not for Pca. Similarly, the development of single nucleotide polymorphism (SNP) markers to conduct genetic studies in Pca has not been implemented, as EST or genomic sequences are not yet available for this pathogen.…”

Section: Pathogenicity and Population Biology Of Pcamentioning

confidence: 99%

Puccinia coronata f. sp. avenae: a threat to global oat production

Nazareno

Smith

et al. 2017

Molecular Plant Pathology

101

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Most crown rust management strategies involve the use of rust-resistant crop varieties and the application of fungicides. The attainment of the durability of resistance against Pca is difficult as it is a highly variable pathogen with a great propensity to overcome the genetic resistance of varieties. Thus, adult plant resistance is often exploited in oat breeding programmes to develop new crown rust-resistant varieties. Useful website: https://www.ars.usda.gov/midwest-area/st-paul-mn/cereal-disease-lab/docs/cereal-rusts/race-surveys/.

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Section: Pathogenicity and Population Biology Of Pcamentioning

confidence: 99%

Puccinia coronata f. sp. avenae: a threat to global oat production

Nazareno

Smith

et al. 2017

Molecular Plant Pathology

101

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“…Genetic analysis of the crown rust-perennial ryegrass interaction has also been limited by the equivocal status of genetic variability among pathogen populations. Substantial evidence has been obtained, however, for the presence of multiple rust pathotypes within and between geographic locations of Australasia and Europe, which are likely to have arisen due to sexual recombination, mutation, and wind-or humanaided spore migration (Dumsday et al, 2003;Aldaoud et al, 2004;Dracatos et al, 2006Dracatos et al, , 2008bDracatos et al, , 2009a.…”

Section: Infection Biology and Life Cycle Of Puccinia Coronata Fsp mentioning

confidence: 99%

Biology and Genetics of Crown Rust Disease in Ryegrasses

et al. 2010

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The most serious foliar disease of perennial ryegrass (Lolium perenne L.) is crown rust (caused by Puccinia coronata Corda f.sp. lolii Brown). Progress in resistance breeding using recurrent selection has been slow, due to lack of genetic knowledge. Puccinia coronata is a basidiomycete fungus with a complex life‐cycle involving both asexual and sexual reproductive modes. Pathotype variation may exist, but confirmation is complicated by the outbreeding nature of the host grass and consequent intrapopulation diversity. Qualitative and quantitative resistance mechanisms may hence be due to either major resistance genes responding to mixed pathogen populations, or minor (quantitative) resistance genes. Advances in molecular biology have permitted analysis of both host and pathogen genetics. Development of pathogen‐derived simple sequence repeat (SSR) genetic markers allowed detailed analysis of genetic variation, as well as generation of homogenized inoculum for detailed trait‐dissection studies. Multiple genomic regions were identified as containing quantitative trait loci (QTLs) for pathogen resistance, and specific single nucleotide polymorphism (SNP) markers for defense response (DR) and disease resistance (R) genes have been evaluated for QTL co‐location. The current knowledge status of the crown rust pathogen and genetics of host resistance is reviewed, as well as future prospects for facilitated rust resistance breeding.

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“…P. coronata f. sp lolli (Dracatos et al, 2006b) and M. linii (Barrett and Brubaker, 2006) primers tested are detailed in Table 2. Polymerase chain reaction (PCR) was carried out in a final volume of 10 µL containing 0.2 mM dNTPs, 1.5 mM MgCl 2 , 1X PCR buffer, 0.25 µM of each primer, 3 U Go Taq Polymerase (Promega) and 30 ng extracted genomic DNA.…”

Section: Pcr Protocolsmentioning

confidence: 99%

Transferability of SSR markers from related Uredinales species to the coffee rust Hemileia vastatrix

Cristancho¹,

Escobar²

2008

Genet. Mol. Res.

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AbSTRACT. The aim of the present research was to test the transferability of simple sequence repeat (SSR) markers developed in two Uredinales species to Hemileia vastatrix, coffee rust. The development of efficient techniques for the identification of H. vastatrix isolates is imperative, given the continuous development of new races. The transferability of 25 SSR markers developed in the related Uredinales species Puccinia coronata f. sp lolli and Melampsora linii to H. vastatrix was tested. A low level of transferability of SSRs was detected, and only 4 potential markers that can be used to fingerprint the coffee rust races were identified.

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Additions and corrections: Development and characterisation of EST-SSR markers from the crown rust pathogen of ryegrass (Puccinia coronata Corda f.sp. lolii)

Cited by 8 publications

References 0 publications

Puccinia coronata f. sp. avenae: a threat to global oat production

Puccinia coronata f. sp. avenae: a threat to global oat production

Biology and Genetics of Crown Rust Disease in Ryegrasses

Transferability of SSR markers from related Uredinales species to the coffee rust Hemileia vastatrix

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