Additions to the genus Chroogomphus (Boletales, Gomphidiaceae) from Pakistan

Abstract

With only three published reports, the genus Chroogomphus (Boletales, Gomphidiaceae) is poorly studied in Pakistan. During recent sampling events in Khyber Pakhtunkhawa province, Pakistan, several collections of Chroogomphus were made, representing undescribed taxa. Based on morphological and molecular data, two new species are described: Chroogomphus pakistanicus and C. pruinosus. We present a description and illustrations for both taxa. A molecular phylogenetic reconstruction, based on the internal transcribed spacer (ITS1–5.8S–ITS2) barcode region, shows that C. pakistanicus and C. pruinosus are placed in two different subgenera of Chroogomphus (subg. Chroogomphus and subg. Siccigomphus, respectively).

New species of Pseudosperma (Agaricales, Inocybaceae) from Pakistan revealed by morphology and multi-locus phylogenetic reconstruction

“…In recent years, many papers have been published, describing new species from different fungal groups collected in Pakistan (e.g. Razaq et al 2012, Nawaz et al 2013, Thongklang et al 2014, Qasim et al 2015a, 2015b, Sarwar et al 2015, Hussain et al 2016, Jabeen et al 2016, Farooqi et al 2017, Naseer et al 2018, Ullah et al 2018, Saba et al 2019a, 2019b, Kiran et al 2020. Thirty-five species of Inocybe sensu lato are reported from Pakistan (Ahmad et al 1997, Ilyas et al 2013, Jabeen et al 2016, Farooqi et al 2017, Razaq and Shahzad 2017, Naseer et al 2018, Ullah et al 2018, Song et al 2019.…”

Two new species of Chroogomphus (Gomphidiaceae, Boletales) with biocultural importance in the Tlahuica-Pjiekakjoo culture from Central Mexico

“…To make the phylogenetic analysis, sequences of previous work were considered including Miller & Aime (2001), Miller et al (2002), Parrent & Vilgalys (2007), Li et al (2009), Kim et al (2015), Scambler et al (2018), Cervini et al (2020, Kiran et al (2020), and GenBank (Table 1). The generated sequences were edited and assembled in the program GENEIOUS PRIME V.2012.2.1(Biomatters Ltd), later they were aligned and the phylogenetic analysis was done in the same program.…”

Delimiting species in Basidiomycota: a review

“…Regarding sequence-based molecular methods, ITS is still the most widely used marker in species delimitation within Boletales (Badotti et al 2017). ITS is a reliable barcode marker for species in several genera, e.g., Astraeus (Phosri et al 2014), Chroogomphus (Scambler et al 2018;Kiran et al 2020), Phylloporus (Neves et al 2012), Rhizopogon (Sulzbacher et al 2016), Suillellus (Vizzini et al 2014c), Suillus (Kretzer et al 1996;Wu et al 2000;Manian et al 2001;Nguyen et al 2016), Xerocomellus (Frank et al 2020), and Xerocomus (Taylor et al 2006a(Taylor et al , b, 2007, but a poor marker in other genera (e.g., Butyriboletus, Pisolithus; Badotti et al 2017). Some genera in Leccinoideae (Leccinum, Octaviania, Rossbeevera and Turmalinea) are characterized by a minisatellite-like insertion within the ITS region (den Bakker et al 2004;Orihara et al 2012).…”