“…A control reaction using heat treated cells afforded no carboxylation product, either, which indicates that a biocatalyst is at work and that the carboxylation is not an unexpected process promoted by non-enzymic constituents of the cell. Extraction Extraction of caffeine from coffee beans [11], extraction of oil from corn fiber [12], extraction of compounds with pharmaceutical importance from microalgae [13], enantioseparation of chiral alcohols by complex formation and subsequent supercritical fluid extraction [14] Chromatography Separation of fatty acids [15], separation of enantiomers [16], supercritical fluid chromatography-mass spectrometry [17], two-dimensional supercritical fluid chromatography [18] Reactions Hydrogenation of supercritical CO 2 to formic acid, alkyl formates, and formamides by homogeneous catalysts [19,20], enantioselective hydrogenation of imines in supercritical CO 2 by iridium-catalyst [21], hydroformylation in supercritical CO 2 by rhodium catalysts [22], asymmetric Diels-Alder reactions in supercritical CO 2 catalyzed by rare earth complexes [23] Other Polymer coating/encapsulation of nanoparticles using a supercritical anti-solvent process [24], improvement of insulin absorption from intratracheally administrated dry powder prepared by a supercritical CO 2 process [25], dyeing of natural fibers from perfluoropolyether reverse micelles in supercritical CO 2 [26] Scheme 1. [49].…”