Additive effect of polymorphisms in the β₂‐adrenoceptor and NADPH oxidase p22 phox genes contributes to the loss of estimated glomerular filtration rate in Chinese

Abstract

Because increased oxidative stress may mediate the detrimental actions of enhanced sympathetic nervous activity on renal function and vice versa, we investigated the effect of the polymorphic Arg16Gly in the β2 -adrenoceptor (ADRB2) gene, Trp64Arg in the β3 -adrenoceptor (ADRB3) gene and C242T in the NADPH oxidase p22phox (CYBA) gene on estimated glomerular filtration rate (eGFR) in a Chinese population. Initially recruited from different outpatient services of HeBei General Hospital in northern China, 668 individuals were finally included in the study, with complete demographic information. Laboratory tests were performed and estimated glomerular filtration rate (eGFR) was derived from the Modification of Diet in Renal Disease (MDRD) equation for the Chinese population. Plasma noradrenaline levels and genotype were determined by HPLC and the TaqMan method, respectively. Only across the Arg16Gly polymorphism did eGFR show significant difference: it was lower in individuals with the Gly16Gly variation, who also had the highest plasma noradrenaline levels. This polymorphism remained a significant determinant of eGFR after multivariate analysis. Of importance, the multifactor dimensionality reduction method further detected a significant synergism between the Arg16Gly and C242T polymorphisms in reducing eGFR. These observations clarify the effects of the studied polymorphisms on eGFR and exemplify gene-gene interactions influencing renal function.

Association of the −449GC and −1151AC polymorphisms in the DDAH2 gene with asymmetric dimethylarginine and erythropoietin resistance in Chinese patients on maintenance hemodialysis

“…17 Statistical analysis was performed by using an SPSS software as described in details in our previous report. 13 Allele frequencies of the −449GC were G 0.519 and C 0.481, and the P values for the Hardy-Weinberg equilibrium were 0.30. Further, the −449GC and −1151AC polymorphisms were in complete linkage disequilibrium as estimated by Lewontin D′ (D′=1.0, P<.001).…”

“…Routine laboratory tests were carried out as previously reported. 13 In addition, plasma ADMA was determined by using high performance liquid chromatography-tandem mass spectrometry. 14 Serum malondialdehyde (MDA) was measured by commercially available kit with the thiobarbituric acid reactive substances method.…”

“…The study protocol was approved by our institutional ethics committee and all participants provided written informed consent.Routine laboratory tests were carried out as previously reported. 13 In addition, plasma ADMA was determined by using high performance liquid chromatography-tandem mass spectrometry. 14…”

“…Routine laboratory tests were carried out as previously reported. 13 In addition, plasma ADMA was determined by using high performance liquid chromatography-tandem mass spectrometry. 14…”

Acute kidney injury in cancer patients and impedance cardiography‑assisted renal replacement therapy: Experience from the onconephrology unit of a Chinese tertiary hospital

“…These subjects were non-diabetic without edema, free of cardiac and renal diseases, devoid of health problems requiring immediate medical attention and received voluntary referral ICG testing. Other selection criteria were similar to those in a previous study by our group (15). Data from these healthy controls were used for establishing the baseline impedance as well as the magnitude and duration of the change in pertinent parameters.…”