Addressing the challenges of non-target feeding studies with genetically engineered plant material – stacked Bt maize and Daphnia magna

Chen, Yi; Romeis, Jörg; Meissle, Michael

doi:10.1016/j.ecoenv.2021.112721

Cited by 5 publications

(19 citation statements)

References 36 publications

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“…One year later at the same time of the year, all the Bt lines and their non-Bt counterparts were grown in the same glasshouse (Y. Chen et al, 2021a).…”

Section: Maize Leaf Powdermentioning

confidence: 99%

“…The limits of detection (LODs) of the test were calculated according to Y. Chen et al (2021a) based on buffer-only blanks of multiple ELISA plates of the same batch of kits.…”

Section: Quantification Of Cry Proteinsmentioning

confidence: 99%

“…For the present study, we used the leaves collected by Y. Chen et al (2021aChen et al ( , 2021b), but we prepared fresh powder from those leaves. The Cry protein concentrations in food suspensions (leaf powder in water) stored in the refrigerator over 6 days remained relatively stable (60%-100% of the Cry protein on day 0).…”

Section: Exposure Of C Riparius To Cry Proteinsmentioning

confidence: 99%

“…Ways to separate plant background effects from Bt effects include: (1) use of the same Bt trait in different plant backgrounds; (2) use of different transformation events with the same Bt protein (e.g., MON810 and Bt11, both expressing the cry1Ab gene); or (3) use of different plant tissues with different concentrations of the Bt protein (e.g., leaves and pollen; Y. Chen et al, 2021a).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the natural range of variation among conventional lines might allow one to judge whether observed differences between a Bt plant and its non-Bt comparator are of biological relevance (Y. Chen et al, 2021aChen et al, , 2021b.…”

Section: Introductionmentioning

confidence: 99%

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No Adverse Effects of Stacked Bacillus thuringiensis Maize on the Midge Chironomus riparius

Chen

Romeis

Meissle

2022

Enviro Toxic and Chemistry

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Material from genetically engineered maize producing insecticidal Cry proteins from Bacillus thuringiensis (Bt) may enter aquatic ecosystems and expose nontarget organisms. We investigated the effects on life table parameters of the midge Chironomus riparius (Diptera: Chironomidae) of SmartStax maize leaves, which contain six different Cry proteins targeting Lepidoptera and Coleoptera pests, in two plant backgrounds. For midge development and emergence, 95% confidence intervals for the means of six conventional maize lines (Rheintaler, Tasty Sweet, ES‐Eurojet, Planoxx, EXP 258, and EXP 262), were used to capture the natural range of variation. For reproduction, lowest and highest means were used. The natural range of variation allows one to judge whether observed effects between Bt maize and the closest non‐Bt comparator are likely to be of biological relevance. No adverse effects on C. riparius were observed with any Bt maize line compared with the respective non‐Bt counterpart. Development time was shorter when females were fed Bt maize than when they were fed non‐Bt maize, but this effect was not considered adverse. Development time, emergence ratio, sex ratio, and larvae/egg rope measured for Bt maize were within the natural range of variation. Fecundity for the Bt lines was equal to or higher than that for the conventional lines. Future risk assessment studies may consider plant background effects and the natural range of variation to judge the relevance of observed differences between particular genetically engineered and non‐genetically engineered plants. Environ Toxicol Chem 2022;41:1078–1088. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

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“…One year later at the same time of the year, all the Bt lines and their non-Bt counterparts were grown in the same glasshouse (Y. Chen et al, 2021a).…”

Section: Maize Leaf Powdermentioning

confidence: 99%

“…The limits of detection (LODs) of the test were calculated according to Y. Chen et al (2021a) based on buffer-only blanks of multiple ELISA plates of the same batch of kits.…”

Section: Quantification Of Cry Proteinsmentioning

confidence: 99%

Section: Exposure Of C Riparius To Cry Proteinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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No Adverse Effects of Stacked Bacillus thuringiensis Maize on the Midge Chironomus riparius

Chen

Romeis

Meissle

2022

Enviro Toxic and Chemistry

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Unintended changes in transgenic maize cause no nontarget effects

Wang

Liu

Song

et al. 2022

Plants People Planet

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Societal Impact Statement Genetic engineering is used to introduce new genes into plants to obtain crops with novel traits. However, the unintended effects of genetically engineered (GE) crops on nontarget organisms—such as beneficial insects—are a topic of much concern. We evaluate the potential unintended effects of pollen from three GE maize and seven commercialized conventional maize lines on the ladybird beetle Propylea japonica, by combining omics approaches with feeding assays. Our results suggest that unintended changes caused by genetic engineering in maize pollen may not lead to biologically relevant effects on P. japonica. Meanwhile our study provides a useful strategy to assess the biological impacts of genetic engineering on nontarget organisms. Summary The potential effects caused by the inserted traits in genetically engineered (GE) plants on nontarget organisms (NTOs) have been well assessed. However, whether the process of genetic engineering itself causes unintended changes that go beyond the natural variation of the crop and further poses any biological effects to NTOs is still under debate. Here, we evaluated the potential unintended effects of pollen from three GE maize and seven commercialized conventional maize lines on the NTO Propylea japonica by combining omics approaches with feeding assays. The results showed that genetic breeding indeed brought somewhat differences at both proteome and metabolome levels in maize pollen, although such differences were far more common in conventionally crossbred plants. Feeding experiments indicated that the changes in proteins and metabolites caused by genetic breeding did not lead to unintended effects on the NTOs that go beyond those measured for the conventional crossbred lines. Together, our results suggest that the differences detected by omics experiments may not cause any biological relevant effects on NTOs and the combination of omics approaches and NTOs feeding assays provide a valid approach to assess the biological relevance of compositional effects caused by genetic breeding.

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No adverse dietary effect of a cisgenic fire blight resistant apple line on the non-target arthropods Drosophila melanogaster and Folsomia candida

Schlathölter

Meissle²,

Boeriis³

et al. 2022

Ecotoxicology and Environmental Safety

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Addressing the challenges of non-target feeding studies with genetically engineered plant material – stacked Bt maize and Daphnia magna

Cited by 5 publications

References 36 publications

No Adverse Effects of Stacked Bacillus thuringiensis Maize on the Midge Chironomus riparius

No Adverse Effects of Stacked Bacillus thuringiensis Maize on the Midge Chironomus riparius

Unintended changes in transgenic maize cause no nontarget effects

No adverse dietary effect of a cisgenic fire blight resistant apple line on the non-target arthropods Drosophila melanogaster and Folsomia candida

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