Adenine nucleotide content and regional function during ischemia and reperfusion in canine ventricular myocardium

Vial, Christian; Crozatier, Bertrand; Goldschmidt, D.; Font, B.

doi:10.1007/bf01908316

Cited by 8 publications

(3 citation statements)

References 29 publications

(28 reference statements)

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“…Glower et al reported 24 hours of recovery following 15 minutes of ischemia in the conscious dog (9). Kloner et al (18) found long lasting dysfunction after a short period of regional ischemia, and Vial et al (36) as well as a recent paper from our laboratory showed a regional dysfunction for several hours in open-chest dog preparations (13). It was demonstrated by Heyndrickx et al (12) that 5 minutes of ischemia are long enough to produce a prolonged postischemic dysfunction.…”

Section: Regional Functionmentioning

confidence: 88%

Repeated short periods of regional myocardial ischemia: effect on local function and high energy phosphate levels

1986

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The effect of recurrent periods of ischemia on the myocardium was investigated in 15 open-chest dogs. Ischemia was produced by 3 minutes of proximal occlusion of the left anterior descending coronary artery. Each occlusion was followed by reperfusion of 3 minutes duration. Forty occlusions with a total of 120 minutes of ischemia were performed, and regional function (sonomicrometry) as well as high energy phosphates (needle biopsies) were determined at the end of the 5th, 20th, and 40th period of ischemia and reperfusion. The first periods of ischemia had a cumulative effect both on regional postischemic function (44% and 59% respectively of preischemic control after 20 occlusions) and on the ATP content, but with increasing number of occlusions the additive effects became smaller (ATP reduction/mumol/g w w/per occlusion). The ATP breakdown per occlusion was diminished with increasing number of periods of ischemia, and no significant adenosine was measured in the ischemic myocardium. Higher than normal postischemic creatine phosphate levels (9.1 mumol/g w w at the 40th reperfusion vs. 6.7 mumol/g w w control) indicated a functioning oxidative phosphorylation in the presence of an ATP utilization problem at the sarcomere level, because indicators of the cellular energy level (energy charge, free energy change of ATP hydrolysis) quickly normalized during reperfusion. Stunned myocardium is therefore not a problem of energy supply but rather of energy utilization. Reduced ATP utilization and regional dysfunction are the expressions of the same cellular defect which resides either in the ATP-splitting contractile apparatus or in the electromechanical coupling. Contractile dysfunction during reperfusion protects the heart against subsequent periods of ischemia because ATP turnover is reduced.

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Section: Regional Functionmentioning

confidence: 88%

Repeated short periods of regional myocardial ischemia: effect on local function and high energy phosphate levels

1986

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“…Recovery of function after cardiac arrest may be influenced independently of the tissue concentration of ATP by a variety of factors, such as the use of normal blood or substrate-enriched blood or perfusate, reduction of Ca 2+ -activity in the perfusate, secondary cardioplegia, or reduction of the after-load in the early recovery phase [17]. During regional ischaemia or post-ischaemic reperfusion, an individual correlation between ATP concentration and segmental function of the myocardium could not be demonstrated [19]. Data are available which show that there is no close relationship between the post-ischaemic ATP concentration and the degree of dysfunction on the one hand, and the rate of recovery of both function and nucleotide pool on the other hand [6].…”

Section: [9]-mentioning

confidence: 99%

Biochemistry: Index of the Functional State of the Heart?

Isselhard¹

1988

British Journal of Anaesthesia

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“…I (1989) function are common findings in reversibly injured myocardium (20,21,36), many authors believe that a causal relationship between these postischemic alterations exists (32,33,39,51). However, there might be only a non-causal coincidence of the time courses in reduction of adeninenucleotides and of basal function (29,30,47,48). Recently a closer relationship between ATP and diastolic function rather than between ATP and systolic function was described (22).…”

Section: Introductionmentioning

confidence: 96%

High-energy phosphates, myocardial contractile function and material properties after short periods of oxygen deficiency

et al. 1989

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To investigate myocardial performance and diastolic properties after repeated periods of oxygen deficiency auxotonic and isovolumic measurements were performed after three periods (4 min) of asphyxia in Wistar rats (n = 19). Additionally, the response of the peak isovolumic left ventricular pressure to postextrasystolic potentiation was measured. The hemodynamic results were compared to the levels of high-energy phosphates. Already after 15 min of recovery from asphyxia auxotonic measures of systolic function were completely normal compared to the control group (n = 19). Isovolumic measurements after 20 min of postasphyctic recovery, however, demonstrated a considerable reduction of the peak left ventricular pressure (226.5 +/- 7.5 mm Hg vs. 262.6 +/- 3.4 mm Hg in controls, mean +/- SEM (p less than 0.01) indicating persistence of decreased postischemic contractile performance. The relative effect of postextrasystolic potentiation was similar in both groups, but could not compensate for the reduced performance of the postasphyctic hearts: the absolute postextrasystolic peak isovolumic pressure of the postasphyctic hearts was lower than the value of the regular isovolumic peak pressure in the controls. Diastolic properties (pressure/volume and stress/strain relationships) of the postasphyctic myocardium remained unchanged. The total sum of the adenine-nucleotides decreased from 7.2 +/- 0.2 to 5.6 +/- 0.3 mumol/gww (p less than 0.01). ATP was reduced from 4.8 +/- 0.2 to 3.9 +/- 0.3 mumol/gww (p less than 0.01). Phosphocreatine was elevated to 7.0 +/- 0.6 mumol/gww, x +/- SEM (p less than 0.01). Our results demonstrated normal postasphyctic basal hemodynamics and material properties. Thus, the energy supply was sufficient to maintain steady state conditions - in spite of decreased overall adenine-nucleotide levels. Isovolumic measurements and postextrasystolic potentation tests, however, indicated that the contractile performance of the postischemic myocardium was still reduced. This functional limitation cannot be explained by altered material properties and is probably not causally related to the decreased overall ATP content.

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Adenine nucleotide content and regional function during ischemia and reperfusion in canine ventricular myocardium

Cited by 8 publications

References 29 publications

Repeated short periods of regional myocardial ischemia: effect on local function and high energy phosphate levels

Repeated short periods of regional myocardial ischemia: effect on local function and high energy phosphate levels

Biochemistry: Index of the Functional State of the Heart?

High-energy phosphates, myocardial contractile function and material properties after short periods of oxygen deficiency

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