Adenine supplement delays senescence in cultured human follicle dermal papilla cells

Cheng, Yi; Young, Guang Huar; Chiu, Tsu Man; Lin, Jiun Tsai; Huang, Pei Ru; Kuo, Cheng Yi; Liang, Yao Jen; Chen, Po Ku; Chen, Sung Fang; Cheng, Ching Yi; Chen, Han Min

doi:10.1111/exd.12881

Cited by 7 publications

(6 citation statements)

References 8 publications

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“…Logically, APRT‐mediated purine salvage is a process of energy consumption, including the synthesis of AMP and the interconversion of adenylate nucleotides by adenylate kinase (Figure 5). 15,50‐52 Previous studies have investigated the effects of adenine supplementation solely to measure the ATP levels and have rarely applied the concept of energy charge to distinguish from interconversion among adenylate nucleotides 53 . In this study, we quantified the time course of adenylate nucleotides in NIH‐3T3 fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Modulation of adenine phosphoribosyltransferase‐mediated salvage pathway to accelerate diabetic wound healing

Young¹,

Lin²,

Cheng³

et al. 2021

FASEB j.

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Adenine phosphoribosyltransferase (APRT) is the key enzyme involved in purine salvage by the incorporation of adenine and phosphoribosyl pyrophosphate to provide adenylate nucleotides. To evaluate the role of APRT in the repair processes of cutaneous wounds in healthy skin and in diabetic patients, a diabetic mouse model (db/db) and age‐matched wild‐type mice were used. Moreover, the topical application of adenine was assessed. In vitro studies, analytical, histological, and immunohistochemical methods were used. Diabetic mice treated with adenine exhibited elevated ATP levels in organismic skin and accelerated wound healing. In vitro studies showed that APRT utilized adenine to rescue cellular ATP levels and proliferation from hydrogen peroxide‐induced oxidative damage. HPLC‐ESI‐MS/MS‐based analysis of total adenylate nucleotides in NIH‐3T3 fibroblasts demonstrated that adenine addition enlarged the cellular adenylate pool, reduced the adenylate energy charge, and provided additional AMP for the further generation of ATP. These data indicate an upregulation of APRT in skin wounds, highlighting its role during the healing of diabetic wounds through regulation of the nucleotide pool after injury. Furthermore, topical adenine supplementation resulted in an enlargement of the adenylate pool needed for the generation of ATP, an important molecule for wound repair.

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Section: Discussionmentioning

confidence: 99%

Modulation of adenine phosphoribosyltransferase‐mediated salvage pathway to accelerate diabetic wound healing

Young¹,

Lin²,

Cheng³

et al. 2021

FASEB j.

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“…In addition, various other compounds have demonstrated stimulatory effect on DPCs at molecular level .…”

Section: Hair Growth Promoting Effectmentioning

confidence: 99%

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

Madaan

Verma

Singh

et al. 2018

Intern J of Cosmetic Sci

126

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Hair disorders such as hair loss (alopecia) and androgen dependent, excessive hair growth (hirsutism, hypertrichosis) may impact the social and psychological well-being of an individual. Recent advances in understanding the biology of hair have accelerated the research and development of novel therapeutic and cosmetic hair growth agents. Preclinical models aid in dermocosmetic efficacy testing and claim substantiation of hair growth modulators. The in vitro models to investigate hair growth utilize the hair follicle Dermal Papilla cells (DPCs), specialized mesenchymal cells located at the base of hair follicle that play essential roles in hair follicular morphogenesis and postnatal hair growth cycles. In this review, we have compiled and discussed the extensively reported literature citing DPCs as in vitro model to study hair growth promoting and inhibitory effects. A variety of agents such as herbal and natural extracts, growth factors and cytokines, platelet-rich plasma, placental extract, stem cells and conditioned medium, peptides, hormones, lipid-nanocarrier, light, electrical and electromagnetic field stimulation, androgens and their analogs, stress-serum and chemotherapeutic agents etc. have been examined for their hair growth modulating effects in DPCs. Effects on DPCs' activity were determined from untreated (basal) or stress induced levels. Cell proliferation, apoptosis and secretion of growth factors were included as primary end-point markers. Effects on a wide range of biomolecules and mechanistic pathways that play key role in the biology of hair growth were also investigated. This consolidated and comprehensive review summarizes the up-to-date information and understanding regarding DPCs based screening models for hair growth and may be helpful for researchers to select the appropriate assay system and biomarkers. This review highlights the pivotal role of DPCs in the forefront of hair research as screening platforms by providing insights into mechanistic action at cellular level, which may further direct the development of novel hair growth modulators.

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“…Logically, APRT-mediated purine salvage is a process of energy consumption, including the synthesis of AMP and the interconversion of adenylate nucleotides by adenylate kinase ( Figure 3) (Cheng, Young et al, 2016. Previous studies investigated the effect of adenine to measure the ATP solely, and rarely applied the concept of energy charge to distinguish from the changes among adenylate nucleotides.…”

Section: Discussionmentioning

confidence: 99%

Modulation of adenine phosphoribosyltransferase-mediated salvage to promote diabetic wound healing

Young¹,

Lin²,

Cheng³

et al. 2020

Preprint

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Adenine phosphoribosyltransferase (APRT) is the key enzyme in purine salvage by the incorporation of adenine and phosphoribosyl pyrophosphate to provide adenylate nucleotide.The up-regulated APRT found in wound skin correlated with the demands of repair in diabetic mice. Administration of adenine on the wound of diabetic mice exhibited elevated ATP levels in organismic skin and accelerated wound healing. In vitro studies showed that APRT utilized adenine to rescue cellular ATP levels and proliferation against hydrogen peroxide-induced oxidative damage. LC-MS/MS-based analysis of total adenylate nucleotides in NIH-3T3 fibroblast showed that adenine addition enlarged the cellular adenylate pool, reduced the adenylate energy charge, and provided more AMP for the generation of ATP in further. These data indicated the role of APRT during diabetic wound healing by regulating the nucleotide pool after injury and demonstrated the improvement by topical adenine, which highlights its value as a promising agent in therapeutic intervention. Our study provided an explanation for the upregulation of APRT in tissue repair and adenine supplement resulted in an enlargement of the adenylate pool for ATP generation.

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Adenine supplement delays senescence in cultured human follicle dermal papilla cells

Abstract: Abbreviations: HFDPCs, Human follicle dermal papilla cells; AMPK, AMP-activated protein kinase; AICAR, Aminoimidazole carboxamide ribonucleotide.

Cited by 7 publications

References 8 publications

Modulation of adenine phosphoribosyltransferase‐mediated salvage pathway to accelerate diabetic wound healing

Modulation of adenine phosphoribosyltransferase‐mediated salvage pathway to accelerate diabetic wound healing

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

Modulation of adenine phosphoribosyltransferase-mediated salvage to promote diabetic wound healing

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