Adenomyosis in the bovine uterus: Correlation between frequency, age, and 17β-estradiol–progesterone equilibrium

Korzekwa, Anna; Bah, Mamadou M.; Gęstwicka, Małgorzata; Socha, Barbara; Skarżyński, Dariusz

doi:10.1016/j.theriogenology.2012.09.023

Cited by 13 publications

(32 citation statements)

References 26 publications

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“…The cows older than 5 years had advanced stages of adenomyosis (approx. 40% of animals; Korzekwa et al 2013). Moreover, the presence of adenomyosis did not differed based on the sampling location in the uterus.…”

Section: Introductionmentioning

confidence: 80%

“…The pathogenesis and aetiology of both endometriosis and adenomyosis are still unclear but during both disorders E2 overproduction has been observed (Takahashi et al 1989, Korzekwa et al 2013). In our study, E2 stimulated Cx43 mRNA expression in myometrial and co-cultured both myometrial and stromal cells collected from adenomyotic cows as well as in co-cultures of cells derived from healthy animals.…”

Section: Discussionmentioning

confidence: 99%

“…These days of the estrous cycle were selected for experiments because this study is continuation of our concerning adenomyosis (Korzekwa et al 2013(Korzekwa et al , 2014(Korzekwa et al , Łupicka et al 2015 and adenomyosis has been observed during the luteal stage of the estrous cycle in cattle (Moreira et al 2007). Uterine tissues were obtained at the Meat Processing Plant "Warmia" (Biskupiec, Poland) and transported on ice to the laboratory within 40 min.…”

Section: Materials Collectionmentioning

confidence: 99%

“…Stained cross-sections of the tissue were observed under a light microscope (Nikon FXA, Tokyo, Japan). Animals were classified as described previously (Korzekwa et al 2013(Korzekwa et al , Łupicka et al 2015; briefly, if uterine glands were present only in the endometrium, and if the endometrial-myometrial border was clearly visible, cows were classified as normal/control. Whereas, if the glands penetrated the myometrial layer of the uterus, animals were classified as adenomyotic (according to the classification of Katkiewicz et al 2005, data not shown).…”

Section: Histochemical Stainingmentioning

confidence: 99%

“…Moreover, the presence of adenomyosis did not differed based on the sampling location in the uterus. The glandular nests in adenomyotic cows were present in myometrial layer of both uterine horns, ipsilateral and contralateral to ovary with corpus luteum (CL), and near the cervix as well (Korzekwa et al 2013(Korzekwa et al , Łupicka et al 2015.…”

Section: Introductionmentioning

confidence: 99%

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Estradiol Reduces Connexin43 Gap Junctions in the Uterus during Adenomyosis in Cows

Korzekwa¹,

Łupicka²,

Socha³

et al. 2016

Polish Journal of Veterinary Sciences

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Adenomyosis is defined as the presence of glandular foci external to the endometrium of the uterus, either in the myometrium or/and perimetrium, depending on the progress of this dysfunction. To date, we showed that steroids secretion and prolactin expression and proliferative processes are disturbed during uterine adenomyosis in cows. During endometriosis in eutopic endometrium in women, gap junctions are down regulated. The transmembrane gap junction protein, connexin (Cx43) is necessary for endometrial morphological, biochemical and angiogenic functions. The aim of this study is recognition of adenomyosis etiology by determination of the role of Cx43 in this process. Immunolocalization and comparison of Cx43 mRNA and protein expression in healthy (N=9) and adenomyotic uterine tissue (N=9), and Cx43 mRNA expression (real time PCR) in uterine stromal -myometrium co-culture under 24-hour stimulation with 17-beta estradiol (10 -7 M) isolated from healthy (N=5) and adenomyotic (N=5) cows were determined. Cx43 was localized in healthy and adenomyotic uteri. mRNA and protein expression was down-regulated in uterine tissue in adenomyotic compared with healthy cows (p<0.05). Estradiol stimulated Cx43 mRNA expression in myometrial cell culture and co-culture of stromal and myometrial cells in adenomyotic compared with healthy cows (p<0.05). In summary, down-regulation of Cx43 expression in the junction zone might play an important role in pathogenesis of adenomyosis. Estradiol modulates gap junctions during adenomyosis.

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Section: Introductionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Materials Collectionmentioning

confidence: 99%

Section: Histochemical Stainingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Estradiol Reduces Connexin43 Gap Junctions in the Uterus during Adenomyosis in Cows

Korzekwa¹,

Łupicka²,

Socha³

et al. 2016

Polish Journal of Veterinary Sciences

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The relationship between bovine adenomyosis and endometritis

et al. 2015

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Adenomyosis is the invasion of endometrium into the myometrium, which could reduce fertility in cows. The aim of the present study was to assess the relationship between the occurrence of adenomyosis and endometritis in dairy cows. Cows in the control group (n=33) had no uterine infection, while cows in the case group (n=37) had endometritis. To harvest uterine specimens, cows in both groups were subjected to uterine biopsy. The uterine specimens were fixed in 10 % formalin, embedded in paraffin, sectioned, stained with hematoxylin and eosin (H&E), and examined histopathologically. Data were analyzed using logistic regression analysis. The prevalence of adenomyosis was 5.4 % (2/37) and 0 % (0/33) in cows with endometritis and control cows, respectively, and there were no significant differences between the two groups (P>0.05). In conclusion, the present study showed a numerically higher prevalence of adenomyosis in cows with endometritis.

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