1973
DOI: 10.1073/pnas.70.4.1099
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Adenosine 3′:5′-Cyclic Monophosphate in Chlamydomonas reinhardtii: Isolation and Characterization

Abstract: Chlamydomonas reinhardtii contains a factor that can replace adenosine 3':5'-cyclic monophosphate (cAMP) in the stimulation of rabbit-muscle protein kinase. The factor cochromatographs and coelectrophoreses with authentic cAMP, and is inactivated by beef heart cyclic nucleotide phosphodiesterase. When C. reinhardtii is exposed to aminophylline (theophylline2 ethylenediamine), the concentration of the factor in the cells increases within 1 hr, from about 25 pmol of cAMP equivalents per g dry weight to more than… Show more

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Cited by 60 publications
(10 citation statements)
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“…The levels of cAMP measured in cell extracts varied between 3 and 3,000 pmol/g fresh weight. The lower value is comparable to the cAMP levels found in ryegrass (3), Funaria (7), and Chlamydomonas (2 (3), and Funaria loses some cAMP when washed (7). Bacterial (17), fungal (13), and animal (5,10) cells are known to release cAMP.…”
Section: Methodsmentioning
confidence: 64%
See 1 more Smart Citation
“…The levels of cAMP measured in cell extracts varied between 3 and 3,000 pmol/g fresh weight. The lower value is comparable to the cAMP levels found in ryegrass (3), Funaria (7), and Chlamydomonas (2 (3), and Funaria loses some cAMP when washed (7). Bacterial (17), fungal (13), and animal (5,10) cells are known to release cAMP.…”
Section: Methodsmentioning
confidence: 64%
“…Strong evidence has been presented for the occurrence of low concentrations of cAMP in sterile cultures of the angiosperm Lolium multflorum (ryegrass) (3), the moss Funaria hygrometrica (7), and the green alga Chlamydomonas reinhardtii (2). The evidence reported here established the presence of high concentrations of cAMP in the chrysophycean alga, Ochromonas malhamensis and in the culture medium in which the cells have grown.…”
mentioning
confidence: 70%
“…Thus RINGO (1967) used the medium of SAGER and GRANICK (1953), OSAFUNE et al (1972) used 3 % CO 2 aerated 3/10 HS-medium (SUEOKA et al 1967) and we have used both the latter medium as well as the TAP-medium of AMRHEIN and FILNER (1973). Both contrast with the 3/10 HS-medium supplemented with 2 % acetate as employed by JOHNSON and PORTER (1968).…”
Section: Discussionmentioning
confidence: 99%
“…There is also no conclusive evidence for the occurrence of either adenyl cyclase or of a phosphodiesterase specific for 3',5'-cyclic nucleotide in higher plants. Cyclic AMP and/or enzymes of its metabolism have so far been recorded only in the green alga C. reinhardtii, fungi, and bacteria (2,5,6,10,23,27,28,30). Cyclic AMP seems to play an important role in regulating the morphogenesis in bacteria and fungi (10,23,26).…”
Section: Discussionmentioning
confidence: 99%
“…In the slime mold Dictyostelium discoideum, cAMP has a morphogenetic effect and is involved in the aggregation of amoebae (10). Among Chl-containing organisms, Chlamydomonas reinhardtii (2) and Euglena gracilis (14) have been found to contain cAMP.…”
mentioning
confidence: 99%