Adenosine Deaminase Activity in Plasma and Blood Cells of Patients with Haematological and Autoimmune Diseases

Storch, H; Krüger, W; Rotzsch, W

doi:10.1159/000207176

Cited by 17 publications

(8 citation statements)

References 10 publications

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“…This was also the case in previous studies (19,20). However, the increase in ADA-2 in our study may suggest that the increase in tADA is due to the increase in ADA-2, and this may indicate that the increase in tADA is probably due to the macrophage activation in addition to T cell activation.…”

Section: Discussionsupporting

confidence: 88%

Serum Adenosine Deaminase and Cytidine Deaminase Activities in Patients with Systemic Lupus Erythematosus

Taysı¹,

Polat²,

Sari³

et al. 2002

Clinical Chemistry and Laboratory Medicine

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We evaluated serum total adenosine deaminase, its isoenzymes adenosine deaminase-1 and adenosine deaminase-2, and cytidine deaminase activities in 24 patients with active systemic lupus erythematosus, and in 26 healthy control subjects, and found the means +/- SD values to be 21.38 +/- 5.96 IU/l, 3.74 +/- 2.12 IU/l, 17.72 +/- 5.02 IU/l and 17.89 +/- 4.62 IU/l, respectively in the patients, and 14.97+/- 4.71 IU/l, 4.01 +/- 1.35 IU/l, 10.91 +/- 3.91 IU/l and 7.39 +/- 3.97 IU/l, respectively in the control subjects. When compared to the healthy controls, serum total adenosine deaminase, adenosine deaminase-2 and cytidine deaminase levels were significantly higher (p<0.001) in systemic lupus erythematosus patients, but the decrease of adenosine deaminase-1 level was not statistically significant (p>0.05). The increased adenosine deaminase-2 may be of macrophage origin. It closely correlated with clinical signs of active systemic lupus erythematosus. The membranes of polymorphonuclear neutrophils may be damaged, and cytidine deaminase may be released into serum. In conclusion, serum total adenosine deaminase, adenosine deaminase-2 and cytidine deaminase activities may serve as useful indicators for evaluating disease activity in patients with active systemic lupus erythematosus.

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Section: Discussionsupporting

confidence: 88%

Serum Adenosine Deaminase and Cytidine Deaminase Activities in Patients with Systemic Lupus Erythematosus

Taysı¹,

Polat²,

Sari³

et al. 2002

Clinical Chemistry and Laboratory Medicine

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“…In this study, ADA activity was demonstrated in plasma, mononuclear cells, and red cells of patients with various types of leukemia and normal subjects, and abnormalities of ADA were detected in these patients as described by others [18][19][20][21][22][23]271. Plasma ADA activity of patients with acute leukemia was especially high in the untreated or relapsed state and normal or low in remission.…”

Section: Discussionsupporting

confidence: 74%

Adenosine deaminase (ADA) in leukemia: Clinical value of plasma ADA activity and characterization of leukemic cell ADA

1985

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Adenosine deaminase (ADA) activity was measured in plasma, erythrocytes, and mononuclear cells from 18 patients with acute and chronic leukemia. High levels of ADA activities were found in plasma, erythrocytes, and mononuclear cells from patients with acute leukemia, especially acute lymphoblastic leukemia, and blastic crisis of chronic myeloid leukemia. Serial determination of plasma ADA activities was done in 9 patients with acute leukemia. All patients untreated or in relapse had an elevation of plasma ADA activity, which decreased to normal or subnormal levels during complete remission. On starch gel electrophoresis, plasma ADA in leukemic patients separated into two bands. The major band showed a mobility identical to that of normal red cells and mononuclear cells, and the minor band corresponded to that of normal plasma ADA. Enzymatic and immunological studies were performed on ADA from leukemic cells of acute myeloid and lymphoblastic leukemia. There were no differences in Michaelis constant for adenosine, thermostability, electrophoretic mobility, immunological reactivity, and specific activity between ADA of leukemic cells and normal mononuclear cells. These results strongly suggest that the increased ADA activity in leukemic cells is caused by an increased synthesis of a structurally normal enzyme and that increased plasma ADA activity in leukemic patients reflects an increment of leukemic cells in bone marrow. Therefore, serial determination of plasma ADA activities seems to provide a good indicator of the total mass of leukemic cells in bone marrow.

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“…Storch et al [33] and Nalini et al [34] have also reported the elevated ADA activity in the patients with systemic rheumatic disease and autoimmune diseases.…”

Section: Discussionmentioning

confidence: 93%

Serum adenosine deaminase activity in patients with systemic lupus erythematosus: a study based on ADA1 and ADA2 isoenzymes pattern

et al. 2011

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Adenosine deaminase (ADA) plays a crucial role in the development and maintenance of normal immune system. So, any immunological imbalances could associate with its altered activity in serum. This study evaluated the activity of total ADA and its isoenzymes in serum of 45 patients with systemic lupus erythematosus (SLE). Included were 23 patients with active SLE, 22 during the inactive phase of the disease, and 45 healthy subjects. Our results provided evidence that the significantly elevated total ADA activity in serum of SLE patients is correlated mainly with the increased ADA2 level. The highest mean ADA2 activity during the relapse phase of the disease could be an indication to the macrophages, the main source of ADA2. It might be concluded that ADA and its isoenzymes analysis in serum of patients could be used as a useful and non-invasive diagnostic tool in evaluation of SLE active phase and the disease severity.

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Adenosine Deaminase Activity in Plasma and Blood Cells of Patients with Haematological and Autoimmune Diseases

Cited by 17 publications

References 10 publications

Serum Adenosine Deaminase and Cytidine Deaminase Activities in Patients with Systemic Lupus Erythematosus

Serum Adenosine Deaminase and Cytidine Deaminase Activities in Patients with Systemic Lupus Erythematosus

Adenosine deaminase (ADA) in leukemia: Clinical value of plasma ADA activity and characterization of leukemic cell ADA

Serum adenosine deaminase activity in patients with systemic lupus erythematosus: a study based on ADA1 and ADA2 isoenzymes pattern

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