Adenosinetriphosphatase activity and the active movements of alkali metal ions

Release of noradrenaline from the cat spleen by sodium deprivation

“…Thus, removal of sodium or its replacement by lithium or choline in the external medium would indirectly depress the activity of the sodiumpotassium pump (Skou, 1957) as the sodium concentration inside the cell is reduced. Since the demonstration by Schatzmann (1953) that cardiac glycosides specifically inhibit cation transport, several investigators have shown their effectiveness against ATPase in a number of organ systems (Skou, 1957;Dunham & Glynn, 1961;Bonting, Caravaggio & Hawkins, 1962). NEM and p-hydroxymercuribenzoate also inhibit ATPase (Skou, 1963;Rendi, 1965;Banerjee, Wong, Khanna & Sen, 1972).…”

The dependence of the respiration of brain cortex on active cation transport

“…This would appear to be the active transport system functioning in the homogenate as an adenosine triphosphatase and thereby stimulating respiration by its generation of ADP. There is, of course, the possibility that other ATPhydrolysing mechanisms besides active transport may also be sensitive to ouabain and sodium but this seems somewhat unlikely in view of the adenosine triphosphatases of erythrocytes (Post, Merritt, Kinsolving & Albright, 1960;Dunham & Glynn, 1961), kidney (Whittam & Wheeler, 1961), brain (Hess & Pope, 1957Jaimefelt, 1961;Deul & Mcllwain, 1961) and crab nerve (Skou, 1957(Skou, , 1960, which are inhibited in fragmented cells by compounds that inhibit active transport in the intact cells. The present results extend these observations on the hydrolysis of ATP in showing that the QO3 is similarly inhibited (by ouabain) and stimulated (by sodium) both in tissue slices and homogenates.…”

Clinical features and biochemical aspects of red blood cells in Hb Hirosaki.

“…ATPase activity of the hemolysate was determined according to Dunhan and Glynn (1961), but using 3.3 mM ATP, 75 mM Na and 50 mM Tris buffer (pH 7.4).…”