1994
DOI: 10.1093/nar/22.22.4779
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Adenovirus 12-mediated down-regulation of the major histocompatibility complex (MHC) class I promoter: identification of a negative regulatory element responsive to Ad12 E1A

Abstract: In highly oncogenic adenovirus (Ad) 12-transformed cells, major histocompatibility complex (MHC) class I gene expression is down-regulated by the products of the viral E1A oncogene at the level of initiation of transcription. However, class I gene expression is unaltered or elevated in non-oncogenic Ad2- or Ad5-transformed cells. These changes in class I expression may permit Ad12-transformed cells to escape host immune surveillance and elicit tumour formation. Here we show that the 2kb of 5' flanking region o… Show more

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Cited by 25 publications
(25 citation statements)
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“…Adenoviruses, another major group of DNA tumour viruses, have been shown to interfere with the expression of the major components of the antigen presentation pathway. The highly oncogenic adenovirus (Ad) 12 E1A represses transcription driven from both the class I heavy chain promoter (Pro tt et al, 1994) and the TAP1/LMP2 bidirectional promoter (Pro tt and Blair, 1997). The E7 protein of the high-risk HPVs exhibits a signi®cant degree of structural and functional similarities to the Ad E1A protein.…”
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confidence: 99%
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“…Adenoviruses, another major group of DNA tumour viruses, have been shown to interfere with the expression of the major components of the antigen presentation pathway. The highly oncogenic adenovirus (Ad) 12 E1A represses transcription driven from both the class I heavy chain promoter (Pro tt et al, 1994) and the TAP1/LMP2 bidirectional promoter (Pro tt and Blair, 1997). The E7 protein of the high-risk HPVs exhibits a signi®cant degree of structural and functional similarities to the Ad E1A protein.…”
mentioning
confidence: 99%
“…A total of 11 mg of DNA was used, comprising 1 mg pRSVluc reference plasmid along with 5 mg of reporter gene plasmid and 5 mg of test vector (or control plasmid). The reporter constructs pH2KCAT (a) and pE2ACAT (b) have been previously described (Pro tt et al, 1994;Dery et al, 1987, respectively) and contain the 2 kb 5' region of the mouse H-2K b gene and the Ad 2 E2 early promoter, respectively, cloned upstream of the chloramphenicol acetyltransferase (CAT) reporter gene. These were co-transfected with the HPV E7 expression plasmids pMo6bE7, pMo16E7 and pMo18E7 that contain the HPV types 6b, 16 and 18 E7 ORFs, respectively (Barbosa et al, 1991), as well as pAsc 4.7, which is the AccI-H fragment of Ad 12 (comprising the E1A coding region plus the 5' end of the E1B gene) cloned between the AccI and EcoRI sites of pBR322 (Byrd et al, 1982).…”
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confidence: 99%
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