Interferon-? and -?, but not tumor necrosis factor-?, demonstrate immunoregulatory effects on carcinoma cell lines infected with human papillomavirus

Bornstein, Jacob; Lahat, Nitza; Kinarty, Amalia; Revel, Michel; Abramovici, H; Shapiro, Sarah

doi:10.1002/(sici)1097-0142(19970301)79:5<924::aid-cncr9>3.0.co;2-b

Cited by 18 publications

(6 citation statements)

References 45 publications

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“…Nevertheless, the intracellular molecular machinery recruited in these changes by TNF-α needs to be studied in the future. Furthermore, the spreading carcinoma cells may have some capability in escaping the immune surveillance since TNF-α has not been found to induce ICAM-1 and HLA class 1 and 2 antigens in SiHa and CaSki cells [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

Diaconu¹,

Rummukainen

Mättö

et al. 2008

BMC Cancer

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Section: Discussionmentioning

confidence: 99%

Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

Diaconu¹,

Rummukainen

Mättö

et al. 2008

BMC Cancer

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“…For example, IFN-Á-induced de novo MHC class II expression was described in normal and malignant cells such as keratinocytes, human osteogenic sarcoma, colorectal carcinoma, and melanoma cell lines (25)(26)(27). Even though the mechanism and the therapeutic value of IFN-Á treatment is not clear, immune-modulatory effects were ascribed to modifications of antigen processing and antigen presentation (28)(29)(30). Furthermore, an improved T cell-mediated killing of pancreatic carcinoma cells was described after IFN-Á stimulation (31).…”

Section: Introductionmentioning

confidence: 99%

Specific immune recognition of pancreatic carcinoma by patient-derived CD4 and CD8 T cells and its improvement by interferon-γ

Schmitz-Winnenthal

Escobedo²,

Beckhove³

et al. 2006

Int J Oncol

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Abstract. Pancreatic carcinoma is a very aggressive disease and little is known about its immunobiology. We here describe the presence in pancreatic cancer patients of spontaneously induced functional CD4 and CD8 memory/effector T cells reactive to autologous tumor cells or to the pancreatic cancer associated antigen, MUC-1. Such specific cells were present in the bone marrow or peripheral blood of most of the 23 tested patients. Low dose stimulation of primary cultures of pancreatic cancer cells with 500 IU/ml IFN-Á for 72 h enhanced HLA-I expression and induced the de novo expression of HLA-II molecules. This led to a much better immune recognition by autologous HLA-I restricted and purified CD8 T cells and allowed tumor cell recognition by HLA-II restricted purified CD4 T-helper cells. Thus, interferon-Á appears to be a useful adjuvant cytokine to enhance the immunogenicity of a patients' tumor cells and their recognition by tumor reactive immune cells.

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“…This aggregation, therefore, may be due to the function of adhesion molecules other than E-cadherin [27]. Constitutive expression of ICAM-1, one of the adhesion molecules, on CaSki cells [28] may partly explain this phenomenon. Incubation of suspended CaSki cells in Ca-KBM also suppressed the DNA ladder.…”

Section: Discussionmentioning

confidence: 99%

Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation

et al. 2007

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Multicellular tumor spheroids (MCTS) are three dimensional cell culture systems induced by suspension culture. MCTS are widely used in cancer research because of their similarity to solid tumors. CaSki cells are derived from a metastatic cervical cancer containing human papillomavirus 16 (HPV16). Cell death of CaSki cells in MCTS has been previously reported, and our model is used to better characterize the mechanisms of cell death of HPV16-positive keratinocytes. In this study, we found that apoptosis of CaSki cells was induced by suspension culture along with the formation of MCTS after 24 h of incubation. In suspended CaSki cells, monoclonal antibodies blocking E-cadherin function inhibited MCTS formation and suppressed suspension-induced apoptosis in a dose-dependent manner. Western blot for E-cadherin detected upregulation of the authentic 120 kDa band from MCTS of CaSki cells as well as a shorter 100 kDa band. Addition of EGF, whose receptor is known to form a complex with E-cadherin, abrogated apoptosis of suspended CaSki cells in a dose-dependent manner. These findings suggest that E-cadherin-dependent cell-cell contact, directly or indirectly, mediates the signal to undergo apoptosis of CaSki cells during MCTS formation, and thus provides new information on the role of E-cadherin in cervical cancer cell apoptosis.

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Interferon-? and -?, but not tumor necrosis factor-?, demonstrate immunoregulatory effects on carcinoma cell lines infected with human papillomavirus

Abstract: The findings of this study point to possible mechanisms leading to suppression of local immune response in the pathogenesis of HPV-associated neoplasia. They also emphasize the complexity of developing an efficient cytokine therapy for patients with premalignant cervical disease.

Cited by 18 publications

References 45 publications

Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

Specific immune recognition of pancreatic carcinoma by patient-derived CD4 and CD8 T cells and its improvement by interferon-γ

Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation

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