Adenovirus‐mediated artificial microRNA against human fibrinogen like protein 2 inhibits hepatocellular carcinoma growth

Wang, Ming; Liu, Junhui; Xi, Dong; Luo, Xiaoping; Ning, Qin

doi:10.1002/jgm.2883

Cited by 16 publications

(15 citation statements)

References 36 publications

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“…These findings are supported by our observation that plasma sFGL2 levels are increased in patients with HCC compared to those without HCC and healthy controls. Either FGL2 knockdown or intratumoral injection of an artificial microRNA targeting hFGL2 leads to a delayed proliferation of tumor cells and an inhibition of tumor growth and angiogenesis as well as improves survival in vivo [ 33 , 38 , 39 ]. In addition, increased FGL2 expression is significantly associated with poor prognosis in patients with ccRCC [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Soluble fibrinogen-like protein 2 levels in patients with hepatitis B virus-related liver diseases

Tong

Hoàn

et al. 2018

BMC Infect Dis

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BackgroundClinical progression of HBV-related liver diseases is largely associated with the activity of HBV-specific T cells. Soluble fibrinogen-like protein 2 (sFGL2), mainly secreted by T cells, is an important effector molecule of the immune system.MethodssFGL2 levels were determined by ELISA assays in sera of 296 HBV patients clinically classified into the subgroups of acute hepatitis B (AHB), chronic hepatitis B (CHB), liver cirrhosis (LC), hepatocellular carcinoma (HCC) and patients with LC plus HCC. As control group, 158 healthy individuals were included. FGL2 mRNA was quantified by qRT-PCR in 32 pairs of tumor and adjacent non-tumor liver tissues.ResultssFGL2 levels were elevated in HBV patients compared to healthy controls (P < 0.0001). In the patient group, sFGL2 levels were increased in AHB compared to CHB patients (P = 0.017). sFGL2 levels were higher in LC patients compared to those without LC (P = 0.006) and were increased according to the development of cirrhosis as staged by Child-Pugh scores (P = 0.024). Similarly, HCC patients had increased sFGL2 levels compared to CHB patients (P = 0.033) and FGL2 mRNA was up-regulated in tumor tissues compared to adjacent non-tumor tissues (P = 0.043). In addition, sFGL2 levels were positively correlated with HBV-DNA loads and AST (Spearman’s rho = 0.21, 0.25 and P = 0.006, 0.023, respectively), but reversely correlated with platelet counts and albumin levels (Spearman’s rho = − 0.27, − 0.24 and P = 0.014, 0.033, respectively).ConclusionssFGL2 levels are induced by HBV infection and correlated with the progression and clinical outcome of HBV-related liver diseases. Thus, sFGL2 may serve as a potential indicator for HBV-related liver diseases.

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Section: Discussionmentioning

confidence: 99%

Soluble fibrinogen-like protein 2 levels in patients with hepatitis B virus-related liver diseases

Tong

Hoàn

et al. 2018

BMC Infect Dis

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“…FGL2 is highly expressed in HCC tissues and contributes to HCC angiogenesis and hypercoagulability 31 . Moreover, FGL2 knockdown delays HCC growth and tumour angiogenesis 12 , 13 . Therefore, we speculate that FGL2 expression is highly associated with renal microthrombosis.…”

Section: Discussionmentioning

confidence: 99%

“…A recent study reported that FGL2 promotes angiogenesis and tumorigenesis in prostate cancer through FGF-2/ERK signalling 11 . Moreover, FGL2 promotes the growth and angiogenesis of human hepatocellular carcinoma (HCC) cells in mouse xenograft injected models 12 , 13 . FGL2 activity increases in B-cell lymphomas but decreases after remission; moreover, detection of FGL2 activity in peripheral blood mononuclear cells (PBMCs) could be a biomarker for B-cell lymphomas 14 .…”

Section: Introductionmentioning

confidence: 99%

Increased expression of Fibrinogen-Like Protein 2 is associated with poor prognosis in patients with clear cell renal cell carcinoma

Tang

Cao

et al. 2017

Sci Rep

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Fibrinogen-like protein 2 (FGL2) is highly expressed in various tumour tissues and plays a vital role in tumour initiation and progression. This study evaluated the clinical significance of FGL2 in patients with clear cell renal cell carcinoma (ccRCC). FGL2 expression in fresh and 170 archived paraffin-embedded ccRCC tissues was measured by quantitative RT-PCR, western blotting, and immunohistochemitry. FGL2 expression was significantly upregulated in ccRCC. Statistical analyses by using Kaplan–Meier method showed that high FGL2 expression was associated with poor overall survival (OS) and recurrence-free survival (RFS) of patients with ccRCC. Multivariate analyses indicated that FGL2 was as an independent prognostic factor of survivaland that tumoural FGL2 levels could significantly predict the prognosis of patients with early-stage ccRCC. Nomogram systems, which integrated FGL2 expression and other clinical parameters, were established and were found to be better than TNM staging in predicting the OS and RFS of patients with ccRCC. FGL2 silencing led to a significant reduction in cells viability and increase in cells apoptosis, accompanied with a reduced ERK1/2 and p38 MAPK activation, in ccRCC cells. Thus, our results suggest that high FGL2 expression is a novel, independent, and an adverse prognostic factor of clinical outcomes in patients with ccRCC.

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“…Interferons, GM-CSF, IL12, CD40L, CTLA4 [156][157][158][159][160][161][162][163][164][165][166][167][168]170] Nucleic-acid-based effectors. Liver detargeting, miR122, miR145, miR148, miR21, let7, KRAS, breast cancer, Hepatocellular carcinoma, colorectal cancer cells [202][203][204][205][206][207][208] c. Selective replication of virus in specific cells.…”

Section: Protein-based Effectorsmentioning

confidence: 99%

Oncolytic Adenoviruses: Strategies for Improved Targeting and Specificity

Hajeri

Sharma

Yamamoto

2020

Cancers

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Cancer is a major health problem. Most of the treatments exhibit systemic toxicity, as they are not targeted or specific to cancerous cells and tumors. Adenoviruses are very promising gene delivery vectors and have immense potential to deliver targeted therapy. Here, we review a wide range of strategies that have been tried, tested, and demonstrated to enhance the specificity of oncolytic viruses towards specific cancer cells. A combination of these strategies and other conventional therapies may be more effective than any of those strategies alone.

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Adenovirus‐mediated artificial microRNA against human fibrinogen like protein 2 inhibits hepatocellular carcinoma growth

Abstract: HFGL2 might be a potential target for controlling angiogenesis in HCC and hfgl2 gene silencing via miRNA may be an alternative strategy for the management of HCC. Copyright © 2016 John Wiley & Sons, Ltd.

Cited by 16 publications

References 36 publications

Soluble fibrinogen-like protein 2 levels in patients with hepatitis B virus-related liver diseases

Soluble fibrinogen-like protein 2 levels in patients with hepatitis B virus-related liver diseases

Increased expression of Fibrinogen-Like Protein 2 is associated with poor prognosis in patients with clear cell renal cell carcinoma

Oncolytic Adenoviruses: Strategies for Improved Targeting and Specificity

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