Adenovirus-Mediated Transduction with Human Glial Cell Line-Derived Neurotrophic Factor Gene Prevents 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine-Induced Dopamine Depletion in Striatum of Mouse Brain

Kojima, Hiroharu; Abiru, Yasuhiro; Sakajiri, Kenichi; Watabe, Kazuhiko; Ohishi, Nobuko; Takamori, Masaharu; Hatanaka, Hiroshi; Yagi, Kazuichi

doi:10.1006/bbrc.1997.7183

Cited by 58 publications

(27 citation statements)

References 28 publications

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“…[11][12][13][14][15][16] Another strategy is to combine both components into one self-regulating virus so that target cells only need to be infected by one virus to allow regulatable expression. 7,[17][18][19][20][21][22][23][24][25][26] Recombinant adeno-associated viral (rAAV) vectors have several properties that make them one of the most promising vehicles for gene delivery to the CNS. 27 These vectors have been reported to infect and transduce both dividing and nondividing cells, including neurons, with minimal cellular toxicity or host immune response.…”

Section: Introductionmentioning

confidence: 99%

Tight regulation from a single tet-off rAAV vector as demonstrated by flow cytometry and quantitative, real-time PCR

et al. 2004

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Vectors suitable for delivery of therapeutic genes to the CNS for chronic neurodegenerative diseases will require regulatable transgene expression. In this study, three self-regulating rAAV vectors encoding humanized green fluorescent protein (hGFP) were made using the tetracycline (tet)-off system. Elements were cloned in different orientations relative to each other and to the AAV internal terminal repeat (ITRs). The advantage of this vector system is that all infected cells will carry both the 'therapeutic' gene and the tet-regulator. To compare the efficiency of the vectors, 293T cells infected by each vector were grown in the presence or absence of the tet-analog doxycycline (dox). Cells were analyzed by flow cytometry for hGFP protein expression, and quantitative RT-PCR (QRT-PCR) for levels of hGFP mRNA and the tet-activator (tTA) mRNA. In the presence of dox, cells infected with one of the vectors, rAAVS3, showed less than 2% total fluorescent intensity and mRNA copy number than cells grown without dox. The other two vectors were significantly more leaky. Levels of tTA mRNA were not affected by dox. The S3 vector also displayed tight regulation in HeLa and HT1080 cells. To assess regulation in the brain, the S3 vector was injected into rat striatum and rats maintained on regular or dox-supplemented water. At 1 month after vector injection, numerous positive cells were observed in rats maintained on regular water whereas only rare positive cells with very low levels of fluorescence were observed in rats maintained on water containing dox. The QRT-PCR analysis showed that dox inhibited expression of hGFP mRNA in brain by greater than 99%. These results demonstrate that exceedingly tight regulation of transgene expression is possible using the tet-off system in the context of a self-regulating rAAV vector and that the specific orientation of two promoters relative to each other and to the ITRs is important. Regulatable vectors based on this design are ideal for therapeutic gene delivery to the CNS.

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Section: Introductionmentioning

confidence: 99%

Tight regulation from a single tet-off rAAV vector as demonstrated by flow cytometry and quantitative, real-time PCR

et al. 2004

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“…Adenoviral-delivered GDNF induced behavioural and neuroprotective effects when injected into the substantia nigra, but not when injected intrastriatally, in rats that had intrastriatal 6-OHDA lesions (Kozlowski et al, 2000). In MPTP-treated mice, adenoviral vector-mediated GDNF delivery to the striatum prevented depletion of striatal dopamine levels (Kojima et al, 1997). A study which compared the effects of intrastriatal and perinigral injection of an adenoviral vector encoding GDNF found that, while both injection routes conferred protective effects on dopaminergic cell bodies in the nigra, only the intrastriatal route reduced motor deficits in rats with intrastriatal 6-OHDA lesions (Connor et al, 1999).…”

Section: Effects Of Gdnf In Vivomentioning

confidence: 95%

The Potential of Neurotrophic Factors for the Treatment of Parkinson’s Disease

Sullivan¹,

Toulouse²

2011

Towards New Therapies for Parkinson's Disease

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“…Adenoviral-delivered GDNF induced behavioural and neuroprotective effects when injected into the substantia nigra, but not into the striatum, in rats that had intrastriatal 6-OHDA lesions [50]. In MPTP-treated mice, adenoviral vector-mediated GDNF delivery to the striatum prevented depletion of striatal dopamine levels [51]. A study which compared the effects of intrastriatal and perinigral injection of an adenoviral vector encoding GDNF found that, while both injection routes conferred protective effects on dopaminergic cell bodies in the nigra, only the intrastriatal route reduced motor deficits, in rats with intrastriatal 6-OHDA lesions [52].…”

Section: Effects Of Gdnf In Vivomentioning

confidence: 97%