Adenylate Cyclase in Gastric Mucosal Biopsies from Patients with Achlorhydria

Becker, Manfred; Miederer, S. E.; Ruoff, H. J.

doi:10.1159/000137873

Cited by 3 publications

(4 citation statements)

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“…GU, and BII patients. The site of the histamine-sensitive enzyme is the parietal cell [1,11,13], and the distinctly shaped concentration-response curves un doubtedly reflect the different parietal cell concentrations as found in the preceding study. Thus, the number of parietal cells per biopsy and assay tube determine the degree of activation.…”

Section: Discussionmentioning

confidence: 62%

“…As with histamine, PGE2 stimulates another gastric mucosal AC-cAMP system which is not lo calized in the parietal cells, and its function is still unknown. From studies with isolated cells of rat [1,8,9], dog [10], and investiga tions in human gastric mucosa [11][12][13], it is now known that the PGE2-sensitive enzyme originates from nonparietal cells. Thus, the present data are interpreted according to the number of parietal and nonparietal cells, as evaluated in the preceeding paper.…”

Section: Discussionmentioning

confidence: 99%

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Morphologically Different Biopsy Specimens of the Human Gastric Mucosa

Leyhe

et al. 1986

Pharmacology

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Adenylate cyclase (AC) in response to prostaglandin E₂ (PGE₂) and histamine was studied in morphologically different biopsy specimens from human gastric mucosa. The activities of the enzyme were log-normally distributed and did not differ between males and females. PGE₂ activated AC in a concentration-dependent manner in normal gastric mucosa (n = 57), chronic superficial gastritis (GI, 18), chronic gastritis with beginning atrophy (GII, 10), chronic atrophic gastritis (GUI, 24), gastric ulcer (GU, 39), duodenal ulcer (DU, 32), and biopsies of patients operated according to Billroth II (BII, 20) and was most efficacious in GUI and BII. Histamine, which was studied in normal gastric mucosa (n = 27), DU (n = 20), GU (n= 13), and BII (n = 18), stimulated AC most efficaciously and potently in DU, was less effective in normal gastric mucosa and GU, and had no effect at all in BII. Cimetidine treatment of DU patients did not change the PGE₂ action, while the degree of stimulation by histamine was reduced. The data indicate characteristic differences of the PGE₂- and histamine-sensitive AC in the mucosal samples of these patients.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Morphologically Different Biopsy Specimens of the Human Gastric Mucosa

Leyhe

et al. 1986

Pharmacology

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“…Previous studies on measuring cyclic AMP production in human gastric biopsies were done exclusively with homogenized tis sue samples [6,9,[18][19][20], Morphological studies and measurement of adenylate cy clase activity could be compared only indi rectly since tissue samples had to be homog enized for measuring adenylate cyclase ac tivity [6,9], Nevertheless the authors have found a clear correlation between morphol ogy and adenylate cyclase activity. In con trast, by our method measurement of cyclic AMP production and morphological studies can be accomplished with the same tissue sample and batch of isolated cells, which enables a direct comparison.…”

Section: Discussionmentioning

confidence: 99%

A Method to Assess Histamine-Induced Cyclic AMP Production in Isolated Gastric Mucosal Cells from Human Biopsies

Sarem-Aslani¹,

Ratge²,

Bigge³

et al. 1991

Pharmacology

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Human gastric mucosal cells were isolated by digestion of fundic biopsies with pronase and collagenase. The mean value of gastric cells per milligram biopsy specimen ± SEM was 59,000 ± 4,300 (n = 31) with a viability of 90 ± 5 %. With the cell yield of 1 patient a series of approximately 70 – 80 cyclic AMP measurements was possible. Histamine stimulated intracellular cyclic AMP production with an EC50 value of 35 ± 25 μmol/l (SEM; n = 4). In the presence of 100 μmol/l histamine the Ki values (μmol/l) for the histamine H2 receptor antagonists averaged 1.45 (cimetidine), 0.10 (ranitidine), and 0.02 (famotidine). No significant inhibition of histamine-induced cyclic AMP production was obtained with the histamine H₁ receptor antagonist triprolidine. With the new method histamine-induced cyclic AMP production can be measured in intact human gastric mucosal cells from fundic biopsy samples.

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“…There is some evidence that cyclic AMP participates in histamine-induced gastric acid secretion (7, 9), whereas its role in acetylcholine-or gastrinmediated acid secretion is still controversial. An unsolved problem is why histamine (10)(11)(12)(13) and pentagastrin (13) stimulate adenylate cyclase in the gastric body mucosa but do not have any effect on the concentration of cyclic AMP (5,10,14,15).…”

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Cyclic AMP and Gastric Secretion in Man: An in Vivo Study in Healthy Volunteers, Duodenal Ulcer Patients, and Pernicious Anaemia Patients

Varis

Raij

Härkönen

et al. 1988

Scandinavian Journal of Gastroenterology

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The metabolism of cyclic AMP and HCl secretion has been studied in eight healthy volunteers, in eight duodenal ulcer (DU) patients, and in four pernicious anaemia patients. Pentagastrin showed a tendency to increase adenylate cyclase and cyclic nucleotide phosphodiesterase activities in the fundal mucosa and caused a significant increase in cyclic AMP output into the gastric juice in healthy volunteers and in DU patients. Cimetidine inhibited all these events but had no effect on basal cyclic AMP output. Vagotomy significantly inhibited basal cyclic AMP output. We conclude that cyclic AMP is involved both in basal and in pentagastrin-stimulated gastric secretion in man. Basal secretion is mainly controlled by vagal tone. The main pathway for this stimulus at the parietal cell may be via other than H2-receptors, probably through acetylcholinergic receptors. However, a significant part of the pentagastrin stimulation of the human parietal cell is via H2-receptors.

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Adenylate Cyclase in Gastric Mucosal Biopsies from Patients with Achlorhydria

Cited by 3 publications

References 1 publication

Morphologically Different Biopsy Specimens of the Human Gastric Mucosa

Morphologically Different Biopsy Specimens of the Human Gastric Mucosa

A Method to Assess Histamine-Induced Cyclic AMP Production in Isolated Gastric Mucosal Cells from Human Biopsies

Cyclic AMP and Gastric Secretion in Man: An in Vivo Study in Healthy Volunteers, Duodenal Ulcer Patients, and Pernicious Anaemia Patients

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