Adenylate Kinase Activity in Cerebrospinal Fluid in Connection with Transitory Ischaemic Attacks

Frithz, Göran; Ericsson, Per; Ronquist, Gunnar

doi:10.3109/03009737709179052

Cited by 15 publications

(3 citation statements)

References 12 publications

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“…The finding is inconsistent with recently published observations (Tarkkanen et al, 1979). If both CK and adenylate kinase levels in CSF (Frithz et al, 1977) need to be measured, they can be obtained simultaneously from one assay if performed without the adenylate kinase inhibitors.…”

contrasting

confidence: 86%

Bioluminescent Assay of Total and Brain‐Specific Creatine Kinase Activity in Cerebrospinal Fluid

Halonen

Nántö

Frey

et al. 1982

Journal of Neurochemistry

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A bioluminescent assay based on the firefly luciferase reaction has been used for determination of creatine kinase activity in CSF. Activities as low as 0.1 U/L can be measured. The coefficient of variation at an activity level of 0.3-0.4 U/L was between 5 and 6%. The assay conditions optimized for serum specimens can be used for CSF. The adenylate kinase activity is almost completely inhibited, which simplifies the procedure. The creatine kinase/(CK) isoenzyme distribution was obtained using the bioluminescent assay in combination with immunoinhibition or ion exchange chromatography. All specimens contained both MM and BB activity, but no MB was found. The study indicates that the bioluminescent assay is useful in the determination of CK isoenzymes in CSF. The clinical importance of the observed CK levels will be reported in a separate communication.

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contrasting

confidence: 86%

Bioluminescent Assay of Total and Brain‐Specific Creatine Kinase Activity in Cerebrospinal Fluid

Halonen

Nántö

Frey

et al. 1982

Journal of Neurochemistry

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“…Recently, an enzyme, adenylate kinase (ATP: AMP phosphotransferase, EC 2.7.4.3), occurring in cerebral tissue but normally not present in CSF in adults (6), was demonstrated to be a sensitive marker of even small and most probably reversible ischaemic changes in brain tissue (3). Adenylate kinase (AK) catalyzes the dismutation reaction of two molecules of adenosine diphosphate (ADP) into one mole cule of adenosine monophosphate (AMP) and one molecule of adenosine triphosphate (ATP).…”

Section: Introductionmentioning

confidence: 99%

Adenylate Kinase Activity and Glutathione Concentration of Cerebrospinal Fluid in Different Neurological Disorders

Ronquist¹,

Frithz²

1979

Eur Neurol

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Adenylate kinase activity and glutathione concentration were measured in cerebrospinal fluid (CSF) of 64 consecutive patients admitted for various neurological disorders. These two analyses were performed in addition to conventional examination of the CSF. Neurological symptoms most probably connected in some cases with no and in others with only subtle changes in the central nervous system were linked to no or only moderate activities of adenylate kinase together with no glutathione. 1 patient with meningioma had no adenylate kinase activity at all while 3 patients with malignant brain tumours showed clear activities similar to 3 patients with well established diagnoses of multiple sclerosis. On the contrary, glutathione was absent in CSF of the patients with brain tumours and multiple sclerosis. Various cerebrovascular diseases involving larger areas of the brain tissue resulted in clear adenylate kinase activities in CSF either alone as in 11 of the patients with cerebral infarction or in combination with the appearance of also glutathione as in the remaining 7 patients with cerebral infarction as well as in the 14 patients studied with hemorrhages of the brain.

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“…Its activity is increased in the cerebrospinal fluid in association with acute cerebrovascular disease, subarachnoid hemorrhage, malignant tumors, and bacterial meningitis [26,80].…”

Section: Cerebrospinal Fluid Enzymesmentioning

confidence: 99%

Nonimmunoglobulin Proteins in Human Cerebrospinal Fluid

Brooks¹

1989

The Cerebrospinal Fluid

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Adenylate Kinase Activity in Cerebrospinal Fluid in Connection with Transitory Ischaemic Attacks

Cited by 15 publications

References 12 publications

Bioluminescent Assay of Total and Brain‐Specific Creatine Kinase Activity in Cerebrospinal Fluid

Bioluminescent Assay of Total and Brain‐Specific Creatine Kinase Activity in Cerebrospinal Fluid

Adenylate Kinase Activity and Glutathione Concentration of Cerebrospinal Fluid in Different Neurological Disorders

Nonimmunoglobulin Proteins in Human Cerebrospinal Fluid

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