Adh enhances Actinobacillus pleuropneumoniae pathogenicity by binding to OR5M11 and activating p38 which induces apoptosis of PAMs and IL-8 release

Wang, Lei; Qin, Wanhai; Zhang, Jing; Bao, Chuntong; Zhang, Hu; Che, Yuanyuan; Sun, Changjiang; Gu, Jingmin; Feng, Xin; Du, Cheng; Wang, Han; Richard, Paul Langford; Lei, Liancheng

doi:10.1038/srep24058

Cited by 23 publications

(15 citation statements)

References 56 publications

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“…In another study, by contrast, a positive correlation between the plasma concentration of IFN-γ at 4 dpi and clinical scores in pigs infected with App was observed ( 5 ). In the present study, no changes in the local lung amounts of IL-10 were observed, which is in accordance with the previous studies ( 5 , 31 ). Contradictory findings between studies, referring to various cytokine profiles, may be due to varying study design ( in vivo vs in vitro ) and the components used for inoculation.…”

Section: Discussionsupporting

confidence: 94%

Pig lung immune cytokine response to the swine influenza virus and the Actinobacillus pleuropneumoniae infection

Czyżewska‐Dors

Dors

Kwit

et al. 2017

Journal of Veterinary Research

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IntroductionThe aim of this study was to evaluate and compare the local innate immune response to the swine influenza virus (SIV) and Actinobacillus pleuropneumoniae (App) infection in pigs.Material and MethodsThe study was performed on 37 seven-week-old pigs, divided into four groups: App-infected (n=11), App+SIV-infected (n=11), SIV-infected (n=11), and control (n=4). Lung samples were collected, following euthanasia, on the 2nd and 4th dpi (three piglets per inoculated group) and on the 10th dpi (remaining inoculated and control pigs). Lung concentrations of IL-1β, IL-6, IL-8, TNF-α, IL-10, IFN-α, and IFN-γ were analysed with the use of commercial porcine cytokine ELISA kits.ResultsLung concentrations of IL-1β, IL-6, IL-8, TNF-α, IFN-α, and IFN-γ were induced in SIV-infected and App+SIV-infected pigs. In the lung tissue of App-infected pigs, only concentrations of IL-1β, IL-6, IL-8, and IFN-γ were elevated. Additionally, in App+SIV-infected pigs, significantly greater concentrations of IL-1β, IL-8, and IFN-α were found when compared with pigs infected with either SIV or App alone. In each tested group, the lung concentration of IL-10 remained unchanged during the entire study.ConclusionThe results of the study indicate that the experimental infection of pigs with SIV or App alone and co-infection with both pathogens induced a local lung inflammatory response. However, the local cytokine response was considerably higher in co-infected pigs compared to single-infected pigs.

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Section: Discussionsupporting

confidence: 94%

Pig lung immune cytokine response to the swine influenza virus and the Actinobacillus pleuropneumoniae infection

Czyżewska‐Dors

Dors

Kwit

et al. 2017

Journal of Veterinary Research

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“…The mice organs were fixed with 4% paraformaldehyde, embedded in paraffin, cut to the slices. HE staining was performed, and the microscopic pathological changes were observed [15], [16].…”

Section: F the Observation Of Organ Lesionsmentioning

confidence: 99%

The Establishment of BALB/c Mouse Model Infected by Salmonella Typhimurium CVCC541

Zhao¹,

Fotina²,

Wang³

et al. 2019

World Journal of Innovative Research

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Pages 33-39 33 www.wjir.org  Abstract-The infection of Salmonella is the main cause for gastrointestinal diseases worldwide. There are about 20 to 40 million cases with gastroenteritis caused by Salmonella infection across the world every year. In our study, we set up the Chinese Salmonella typhimurium CVCC541 infected mouse model by intraperitoneal injection with different amount of bacteria respectively. The results suggested the severity of clinical symptoms in mice was positively correlated with the dosage and duration of infection. Mice infected by S. typhimurium CVCC541 behaved loose coat and hunched back, sluggish, anorexic, and tendency to gather together. The necropsy results showed the severity of pathological changes was also positively correlated with the dosage of infection. The major lesion occurred in the intestine. Severe hemorrhage in the intestinal tract, especially in jejunum and ileum. Meanwhile, we found the amount of neutrophils increased, and the amounts of macrophages and T lymphocytes decreased significantly in the spleen of mice infected with S. typhimurium CVCC541. We first reported the clinical symptoms and pathological damages of mice caused by the infection of S. typhimurium CVCC541 at various concentrations in detail, which would provide the reference for studies of the molecular mechanism of Salmonella infection and drug resistance.

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“…In addition, we found that the clinical symptoms of mice infected by APP are similar to those of infected piglets when we researched the APP virulence factor ADH. This result indicates that the results obtained from the mouse model played an important role in guiding APP infection research in pigs (Wang et al 2016). Therefore, a mouse model can provide an important reference and information for researching the pathogenesis mechanisms of APP and developing vaccines against APP.…”

Section: Discussionmentioning

confidence: 83%

Recombinant tandem epitope vaccination provides cross protection against Actinobacillus pleuropneumoniae challenge in mice

et al. 2020

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Actinobacillus pleuropneumoniae (A. pleuropneumoniae/APP) is the pathogen that causes porcine contagious pleuropneumonia. Actinobacillus pleuropneumoniae is divided into 18 serovars, and the cross protection efficacy of epitopes is debatable, which has resulted in the slow development of a vaccine. Consequently, epitope-based vaccines conferring Actinobacillus pleuropneumoniae cross protection have rarely been reported. In this study, B cell epitopes in the head domain of trimeric autotransporter adhesin were predicted, and 6 epitopes were selected. Then, the predicted epitopes (Ba1, Bb5, C1, PH1 and PH2) were connected by linkers to construct a recombinant tandem antigen (rta) gene. The RTA protein encoded by the recombinant rta gene was expressed, and finally the ICR mice were immunized with the RTA protein with or without inactivated Actinobacillus pleuropneumoniae (serovars 1 and 5b) and challenged with Actinobacillus pleuropneumoniae to evaluate the protective effect of the epitope-based vaccine and combined vaccine. The mice in the RTA-immunized group and RTA plus inactivated Actinobacillus pleuropneumoniae vaccine group had a significant improvement in clinical symptoms and a higher level of antibody in the serum than those in the control group. The RTA immune group had a 40% survival rate after Actinobacillus pleuropneumoniae infection, whereas the combination of RTA and inactivated Actinobacillus pleuropneumoniae produced very strong cross immune protection in mice, at least 50% (RTA IB1 + C5) and at most 100% (RTA IB5 + C1), whereas no cross immunoprotection was found in the solo Actinobacillus pleuropneumoniae immune group. Overall, the combination of the RTA protein and inactivated bacteria significantly enhanced the cross protection effects. This implies that RTA protein in combination with a suitable inactivated Actinobacillus pleuropneumoniae strain could be a candidate vaccine for porcine contagious pleuropneumonia.

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Adh enhances Actinobacillus pleuropneumoniae pathogenicity by binding to OR5M11 and activating p38 which induces apoptosis of PAMs and IL-8 release

Cited by 23 publications

References 56 publications

Pig lung immune cytokine response to the swine influenza virus and the Actinobacillus pleuropneumoniae infection

Pig lung immune cytokine response to the swine influenza virus and the Actinobacillus pleuropneumoniae infection

The Establishment of BALB/c Mouse Model Infected by Salmonella Typhimurium CVCC541

Recombinant tandem epitope vaccination provides cross protection against Actinobacillus pleuropneumoniae challenge in mice

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