The Establishment of BALB/c Mouse Model Infected by Salmonella Typhimurium CVCC541

Abstract

Pages 33-39 33 www.wjir.org  Abstract-The infection of Salmonella is the main cause for gastrointestinal diseases worldwide. There are about 20 to 40 million cases with gastroenteritis caused by Salmonella infection across the world every year. In our study, we set up the Chinese Salmonella typhimurium CVCC541 infected mouse model by intraperitoneal injection with different amount of bacteria respectively. The results suggested the severity of clinical symptoms in mice was positively correlated with the dosage and duration of infection. Mice infected by S. typhimurium CVCC541 behaved loose coat and hunched back, sluggish, anorexic, and tendency to gather together. The necropsy results showed the severity of pathological changes was also positively correlated with the dosage of infection. The major lesion occurred in the intestine. Severe hemorrhage in the intestinal tract, especially in jejunum and ileum. Meanwhile, we found the amount of neutrophils increased, and the amounts of macrophages and T lymphocytes decreased significantly in the spleen of mice infected with S. typhimurium CVCC541. We first reported the clinical symptoms and pathological damages of mice caused by the infection of S. typhimurium CVCC541 at various concentrations in detail, which would provide the reference for studies of the molecular mechanism of Salmonella infection and drug resistance.

Celastrol mitigates staphyloxanthin biosynthesis and biofilm formation in Staphylococcus aureus via targeting key regulators of virulence; in vitro and in vivo approach

“…Interestingly, organ weight increased significantly after bacterial challenge with untreated pigmented S. aureus in comparison to control uninfected mice, which could be attributed to inflammtory response accompanied with bacterial colonization. These findings are completely in agreement with previous studies [76,77] that reported that mice inoculation with virulent bacteria results in increased organ weight relative to control uninfected mice. Both celastrol treated and non-pigmented S. aureus were less virulent in mice and thus less able to colonize mouse organs than untreated pigmented bacteria.…”