Adherence of Aeromonas caviae to human cell lines Hep-2 and Caco-2

Thornley, Jonathan; Shaw, Jonathan G.; Gryllos, Ioannis; Eley, A.

doi:10.1099/00222615-45-6-445

Cited by 35 publications

(41 citation statements)

References 30 publications

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“…The adhesion of aeromonads to intestinal cells in vivo would be considered a crucial early step in the development of gastro-enteritis. Virulent strains of A. caviae are adherent to HEp-2 cells [20,46] and all the 13 A. caviae diarrhoeal isolates in the present study adhered to the rabbit small intestinal surface, and various values of adherence index were obtained. Dickson and Koohmaraie [42] reported a similar observation.…”

Section: Enhancement Of a Caviae Virulence 307mentioning

confidence: 96%

Enhancement of the virulence of Aeromonas caviae diarrhoeal strains by serial passages in mice

Krzymińska¹,

Mokracka²,

Łaganowska³

et al. 2001

Journal of Medical Microbiology

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Thirteen clinical Aeromonas caviae isolates from the faeces of 13 children with mild to severe diarrhoea were tested for enhancement of mouse lethality, adhesion ability, siderophore and cholera toxin cross-reactive (CTC) factor production, by four consecutive passages through mice by intraperitoneal injection of A. caviae suspensions. The passaged A. caviae strains were re-isolated from monomicrobic cardiac blood samples and inocula were prepared for the next passage. All A. caviae isolates possessed the ability to adhere to the mucosal epithelial surface of the rabbit small intestine. Serial passage in mice showed that the virulence of some isolates for mice was increased in terms of percentage mortality and a lowering of the LD50. For some of the isolates, but not all, serial passage appeared to increase siderophore production and adhesion to rabbit small intestinal cells. For the A. caviae isolates tested, increased values of the CTC factor were observed after passage. A clear correlation was observed between the lowering of LD50 and the enhancement of CTC factor production after passage in mice. These results indicate that the A. caviae isolates possessed virulence factors.

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Section: Enhancement Of a Caviae Virulence 307mentioning

confidence: 96%

Enhancement of the virulence of Aeromonas caviae diarrhoeal strains by serial passages in mice

Krzymińska¹,

Mokracka²,

Łaganowska³

et al. 2001

Journal of Medical Microbiology

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“…Filamentous structures have been implicated, but adherence-related extracellular appendages have not yet been isolated or characterised from A . caviae [ 10,111. This study examined diarrhoeaassociated isolates of A .…”

Section: Introductionmentioning

confidence: 99%

Characterisation of a type IV pilus produced by Aeromonas caviae

Kirov

Sanderson²,

Dickson³

1998

Journal of Medical Microbiology

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A pilus produced by a clinical isolate of Aeromanas caviae (strain CA195) was purified and partially characterised. The M, of the pilin was estimated to be 23 kDa by SDS-PAGE. Its N-terminal amino-acid sequence showed that it was closely related to 'bundleforming' type IV pili purified from other Aeromonas spp. associated with gastro-enteritis and considered to be important intestinal colonisation factors. Bundle-forming pili, often with a polar location, were seen on the surface of strain CA195 which was highly adherent to HEp-2 cells. Removal of surface structures by mechanical means decreased adhesion (by 2 50%) suggesting that these pili played some role in HEp-2 cell binding. This pilus type could prove an important marker for enteropathogenic A. caviae which appear to lack other putative virulence factors.

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“…Only five of the reference strains for hybridization groups, corresponding to groups 4, 5a, 6, 9, and 12, exhibited a positive hybridization reaction. Of the reference strains used for O serotyping (44 strains), 47% showed positive hybridization reactions, and these strains corresponded to serotypes 2,3,7,8,9,10,11,12,14,16,17,19,25,26,28,30,38,39,40,42, and 44. The representative Aeromonas strains A. caviae Sch3N and A. veronii biovar sobria AH-1 hybridized positively to all lateral flagellar probes.…”

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confidence: 99%

Analysis of the Lateral Flagellar Gene System ofAeromonas hydrophilaAH-3

et al. 2006

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Mesophilic Aeromonas strains express a polar flagellum in all culture conditions, and certain strains produce lateral flagella on semisolid media or on surfaces. Although Aeromonas lateral flagella have been described as a colonization factor, little is known about their organization and expression. Here we characterized the complete lateral flagellar gene cluster of Aeromonas hydrophila AH-3 containing 38 genes, 9 of which (lafA-U) have been reported previously. Among the flgL L and lafA structural genes we found a modification accessory factor gene (maf-5) that is involved in formation of lateral flagella; this is the first time that such a gene has been described for lateral flagellar gene systems. All Aeromonas lateral flagellar genes were located in a unique chromosomal region, in contrast to Vibrio parahaemolyticus, in which the analogous genes are distributed in two different chromosomal regions. In A. hydrophila mutations in flhA L , lafK, fliJ L , flgN L , flgE L , and maf-5 resulted in a loss of lateral flagella and reductions in adherence and biofilm formation, but they did not affect polar flagellum synthesis. Furthermore, we also cloned and sequenced the A. hydrophila AH-3 alternative sigma factor 54 (rpoN); mutation of this factor suggested that it is involved in expression of both types of flagella.Mesophilic Aeromonas strains are ubiquitous waterborne bacteria and pathogens of reptiles, amphibians, and fish (4). They can be isolated as part of the fecal flora of a wide variety of other animals, including some animals consumed by humans, such pigs, cows, sheep, and poultry. In humans, Aeromonas hydrophila strains belonging to hybridization group 1 (HG1) and HG3, Aeromonas veronii biovar sobria (HG8/ HG10), and Aeromonas caviae (HG4) have been associated with gastrointestinal and extraintestinal diseases, such as wound infections, and less commonly with septicemias of immunocompromised patients (14). The pathogenicity of mesophilic aeromonads has been linked to a number of different determinants, such as toxins, proteases, outer membrane proteins (28), lipopolysaccharide (23), and flagella (23, 32).Mesophilic Aeromonas strains usually have a single polar unsheathed flagellum in all culture conditions, but it is known that 50% to 60% of the mesophilic aeromonads most commonly associated with diarrhea (18) also have many unsheathed peritrichous lateral flagella when they are grown in viscous environments or on surfaces (38). Different workers have shown that lateral flagella increase bacterial adherence and are required for swarming motility and biofilm formation (10, 19).The expression of two distinct flagellar systems is relatively uncommon, although it has been observed in Vibrio parahaemolyticus (19), Azospirillum brasilense (26), Rhodospirillum centenum (15), Helicobacter mustelae (29), and Plesiomonas shigelloides (13). V. parahaemolyticus is the best-studied organism, and it has two distinct flagellar systems. Recently, an Escherichia coli O42 lateral flagellar gene cluster (Flag-2) has been d...

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Adherence of Aeromonas caviae to human cell lines Hep-2 and Caco-2

Cited by 35 publications

References 30 publications

Enhancement of the virulence of Aeromonas caviae diarrhoeal strains by serial passages in mice

Enhancement of the virulence of Aeromonas caviae diarrhoeal strains by serial passages in mice

Characterisation of a type IV pilus produced by Aeromonas caviae

Analysis of the Lateral Flagellar Gene System ofAeromonas hydrophilaAH-3

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