Adherence of Candida albicans to human buccal epithelial cells

Kimura, Lucille H.; Pearsall, Nancy N.

doi:10.1128/iai.21.1.64-68.1978

Cited by 210 publications

(77 citation statements)

References 14 publications

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“…Cell separation and adherence assays were modified from protocols established by Kimura and Pearsall [27] and Sobell et al [28] as follows. Yeast cells were grown on malt agar (Difco) for 36 h at 37°C, re-suspended in 2 ml of sterile 0.1 mol l )1 phosphate-buffered saline (PBS) pH 6.8 at 4°C, washed twice by centrifugation (2500 g, 5 min each) with 2 ml of PBS and finally re-suspended in PBS (2 · 10 7 cells ml )1 ).…”

Section: Cells and The Adherence Assaymentioning

confidence: 99%

Adherence of Candida albicans and Candida parapsilosis to epithelial cells correlates with fungal cell surface carbohydrates

Lima-Neto

Beltrão²,

Oliveira

et al. 2010

Mycoses

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Many studies have described the adherence of Candida albicans to epithelial cells but little is known about Candida parapsilosis adhesion and its role in host cell surface recognition. This study was designed to evaluate the correlation between the adherence of 20 C. albicans and 12 C. parapsilosis strains to human buccal epithelial cells and the expression of fungal cell surface carbohydrates using lectin histochemistry. Adherence assays were carried out by incubating epithelial cells in yeast suspensions (10(7) cells ml(-1) ) and peroxidase conjugated lectins (Con A, WGA, UEA I and PNA at 25 μg ml(-1) ) were used for lectin histochemistry. The results showed that adherence was overall greater for C. albicans than for C. parapsilosis (P < 0.01) and that the individual strain differences correlated with a high content of cell surface α-l-fucose residues as indicated by the UEA I staining pattern. Based on the saccharide specificity of the lectins used, these results suggest that l-fucose residues on cell surface glycoconjugates may represent recognition molecules for interactions between the yeast strain studied and the host (r = 0.6985, P = 0.0045). In addition, our results indicated the presence of α-d-glucose/α-d-mannose, N-acetyl-D-glucosamine/N-acetylneuraminic acid and D-galactose/N-acetyl-D-galactosamine in fungal cell wall.

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Section: Cells and The Adherence Assaymentioning

confidence: 99%

Adherence of Candida albicans and Candida parapsilosis to epithelial cells correlates with fungal cell surface carbohydrates

Lima-Neto

Beltrão²,

Oliveira

et al. 2010

Mycoses

View full text Add to dashboard Cite

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“…The method of Kimura and Persall [22] was used with slight modi¢cations [21]. Suspensions of human buccal epithelial cells (1U10 S cells ml 3I in PBS, 0.1 ml) and yeast cells (1U10 V cells ml 3I in PBS, 0.1 ml) were mixed and incubated at 37³C for 45 min with gentle shaking.…”

Section: Adherence To Buccal Epithelial Cellsmentioning

confidence: 99%

Altered adherence in strains of Candida albicans harbouring null mutations in secreted aspartic proteinase genes

Watts¹

1998

FEMS Microbiology Letters

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The aspartate proteinase inhibitor pepstatin A has been shown previously to reduce the adherence of Candida albicans yeast cells to human surfaces. This suggests that in addition to their presumed function facilitating tissue penetration, the secreted aspartate proteinases (Saps) of this fungal pathogen may have auxiliary roles as cellular adhesins. We therefore examined the relative adherence of yeast cells of a parental wild-type strain of C. albicans in relation to yeast cells of strains harbouring specific disruptions in various members of the SAP gene family in an otherwise isogenic background. The adhesiveness of vsap1, vsap2 and vsap3 null mutants and a triple vsap 4^6 disruptant was examined on three surfaces^glass coated with poly-L-lysine or a commercial cell-free basement membrane preparation (Matrigel) and on human buccal epithelial cells. Pepstatin A reduced adherence to all surfaces. Adherence of the each of the single SAP null mutants to these three substrates was either reduced or not affected significantly compared to that of the parental strain. The adherence of the vsap4^6 mutant was reduced on poly-L-lysine and Matrigel, but increased on buccal cells. The results suggest that in addition to a primary enzymatic role, various SAPs may also act singly or synergistically to enhance the adhesiveness to C. albicans cells to certain human tissues. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

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“…The attachment assay was performed by a modification of the method described by Kimura and Pearsall [5]. Duplicate samples of each control and test were prepared by mixing equal volumes (100 #l/ml) of the BEC and the blastospores.…”

Section: Methodsmentioning

confidence: 99%

Lewis a blood group antigen of non-secretors: a receptor for candida blastospores

May

1989

FEMS Microbiology Letters

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Adherence of Candida albicans to human buccal epithelial cells

Cited by 210 publications

References 14 publications

Adherence of Candida albicans and Candida parapsilosis to epithelial cells correlates with fungal cell surface carbohydrates

Adherence of Candida albicans and Candida parapsilosis to epithelial cells correlates with fungal cell surface carbohydrates

Altered adherence in strains of Candida albicans harbouring null mutations in secreted aspartic proteinase genes

Lewis a blood group antigen of non-secretors: a receptor for candida blastospores

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