Adherence of Mycoplasma bovis to bovine bronchial epithelial cells

Thomas, Anne; Sachse, Konrad; Farnir, Frédéric; Dizier, Isabelle; Mainil, Jacques; Lindén, Annick

doi:10.1016/s0882-4010(03)00003-2

Cited by 32 publications

(27 citation statements)

References 16 publications

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“…These results are contradictory to the findings obtained using a bovine bronchial epithelial cell line, where the use of monoclonal antibodies to VspF significantly reduced the adherence of M. bovis (Thomas et al, 2003). However, it is important to note that the monoclonal used in the study may show cross-reactivity against other surface lipoproteins and that it was not tested if the M. bovis strain used was expressing VspF (Thomas et al, 2003). Previous studies have also shown a link between the expression of specific lipoproteins and adhesion for M. bovis.…”

Section: Discussioncontrasting

confidence: 99%

Biofilm formation by mycoplasma species and its role in environmental persistence and survival

McAuliffe¹,

Ellis

Miles³

et al. 2006

Microbiology

168

160

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Although mycoplasmas possess a very limited genome, little is known about their virulence mechanisms and methods of persistence in the host. Examination of a wide range of mycoplasma species found considerable variation in their ability to form a biofilm. Mycoplasma putrefaciens, M. cottewii, M. yeatsii, M. agalactiae and M. bovis produced prolific biofilms. Conversely, the highly pathogenic mycoplasma and causative agent of contagious bovine pleuropneumonia, Mycoplasma mycoides subsp. mycoides SC, was unable to produce a biofilm. Biofilms were found to be considerably more resistant to stress, including heat and desiccation, than planktonic cells. A link between the biofilm phenotype and genotype as determined by molecular typing was found for M. bovis. Analysis of biofilms using fluorescent staining combined with confocal microscopy demonstrated that mycoplasma biofilms formed a highly differentiated structure with stacks and channels. Biofilm formation may indicate that mycoplasmas are capable of surviving in the environment.

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Section: Discussioncontrasting

confidence: 99%

Biofilm formation by mycoplasma species and its role in environmental persistence and survival

McAuliffe¹,

Ellis

Miles³

et al. 2006

Microbiology

168

160

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“…Extension of these findings to the M. gallisepticum chicken infection model demonstrated a correlation between the in vitro invasive potential of specific mycoplasma strains and their mucosal penetration and virulence in vivo (Much et al, 2002). Thomas et al (2003) described varied adherence rates when Mycoplasma bovis isolates that differed in the number of in vitro passages were used to infect a variety of cultured host cells. Despite past precedent for altered host function as a consequence of laboratory passage, we observed little difference between the high-passage G37 and low-passage 1019V strains using the cellular interaction phenotype of nuclear localization.…”

Section: Discussionmentioning

confidence: 83%

Interaction of Mycoplasma genitalium with host cells: evidence for nuclear localization

et al. 2008

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Mycoplasma genitalium (Mg) is a mollicute that causes a range of human urogenital infections. A hallmark of these bacteria is their ability to establish chronic infections that can persist despite completion of appropriate antibiotic therapies and intact and functional immune systems. Intimate adherence and surface colonization of mycoplasmas to host cells are important pathogenic features. However, their facultative intracellular nature is poorly understood, partly due to difficulties in developing and standardizing cellular interaction model systems. Here, we characterize growth and invasion properties of two Mg strains (G37 and 1019V). Mg G37 is a high-passage laboratory strain, while Mg 1019V is a low-passage isolate recovered from the cervix. The two strains diverge partially in gene sequences for adherence-related proteins and exhibit subtle variations in their axenic growth. However, with both strains and consistent with our previous studies, a subset of adherent Mg organisms invade host cells and exhibit perinuclear targeting. Remarkably, intranuclear localization of Mg proteins is observed, which occurred as early as 30 min after infection. Mg strains deficient in adherence were markedly reduced in their ability to invade and associate with perinuclear and nuclear sites.

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“…The upper respiratory tract of young animals is colonized at a very early age by contact with shedding animals and also by ingestion of contaminated milk (reviewed in [4]). Adhesion to epithelial cells aids in the colonization but the adhesion capacity varies between isolates [7]. The variable-surface proteins (Vsps) and other surface proteins have been associated with attachment to host cells [7,8].…”

Section: The Challengesmentioning

confidence: 99%

“…Adhesion to epithelial cells aids in the colonization but the adhesion capacity varies between isolates [7]. The variable-surface proteins (Vsps) and other surface proteins have been associated with attachment to host cells [7,8]. One complicating factor is that the capacity of Vsps for phase and antigenic variation [9].…”

Section: The Challengesmentioning

confidence: 99%