Adherence of Serratia marcescens in the pathogenesis of urinary tract infections in diabetic mice

Obana, Yoshiki; Shibata, Kaneyoshi; Nishino, Takeshi

doi:10.1099/00222615-35-2-93

Cited by 12 publications

(4 citation statements)

References 8 publications

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“…After 6 days of alloxan administration, animals with fasted blood glucose level >250 mg/dL were used for in vivo experiments [36,37]. The diabetic mice were fasted overnight prior to the treatment and remained fasted for another 12 h during the experiment, but only animals were allowed water ad labitum.…”

Section: In-vivo Pharmacological Response Of Chitosan/insulin Self-asmentioning

confidence: 99%

Oral insulin delivery by self-assembled chitosan nanoparticles: In vitro and in vivo studies in diabetic animal model

Mukhopadhyay

Chakraborty

Bhattacharya

et al. 2013

Materials Science and Engineering: C

166

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Section: In-vivo Pharmacological Response Of Chitosan/insulin Self-asmentioning

confidence: 99%

Oral insulin delivery by self-assembled chitosan nanoparticles: In vitro and in vivo studies in diabetic animal model

Mukhopadhyay

Chakraborty

Bhattacharya

et al. 2013

Materials Science and Engineering: C

166

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“…Others, such as the anaerobe C. perfringens, are thought to contribute to synergy by secreting powerful cytotoxins that disrupt the immune system (36). Diabetic mice have been used to study the effects of diabetes on periodontitis (18), urinary tract infections (33), septicemia (25), and lung infections (32). Bessman et al (2) used a strain of mice, C57B1.Ks-J-db-m (now named BKS.Cg-m ϩ/ϩ Lepr db / J), which is homozygous for the diabetes spontaneous mutation in the leptin receptor and is a model of type 2 diabetes (see reference 12 and http://jaxmice.jax.org/jaxmice-cgi/jaxmicedb .cgi?objtypeϭpricedetail&stockϭ000642 for a description of the strain), to examine abscess formation and bacterial load after subcutaneous inoculation of E. coli, B. fragilis, and enterococcus.…”

mentioning

confidence: 99%

Synergy in Polymicrobial Infections in a Mouse Model of Type 2 Diabetes

Mastropaolo¹,

Evans

Byrnes

et al. 2005

Infect Immun

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Human diabetics frequently suffer delayed wound healing, increased susceptibility to localized and systemic infections, and limb amputations as a consequence of the disease. Lower-limb infections in diabetic patients are most often polymicrobial, involving mixtures of aerobic, facultative anaerobic, and anaerobic bacteria. The purpose of this study is to determine if these organisms contribute to synergy in polymicrobial infections by using diabetic mice as an in vivo model. The model was the obese diabetic mouse strain BKS.Cg-m ؉/؉ Lepr db /J, a model of human type 2 diabetes. Young (5-to 6-week-old) prediabetic mice and aged (23-to 24-week-old) diabetic mice were compared. The mice were injected subcutaneously with mixed cultures containing Escherichia coli, Bacteroides fragilis, and Clostridium perfringens. Progression of the infection (usually abscess formation) was monitored by examining mice for bacterial populations and numbers of white blood cells at 1, 8, and 22 days postinfection. Synergy in the mixed infections was defined as a statistically significant increase in the number of bacteria at the site of injection when coinfected with a second bacterium, compared to when the bacterium was inoculated alone. E. coli provided strong synergy to B. fragilis but not to C. perfringens. C. perfringens and B. fragilis provided moderate synergy to each other but only in young mice. B. fragilis was anergistic (antagonistic) to E. coli in coinfections in young mice at 22 days postinfection. When age-matched nondiabetic mice (C57BLKS/J) were used as controls, the diabetic mice exhibited 5 to 35 times the number of CFU as did the nondiabetic mice, indicating that diabetes was a significant factor in the severity of the polymicrobial infections.

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“…The symptoms of S. marcescens infection may include fever, chills, diarrhea, and abdominal cramps [ 3 , 7 ]. S. marcescens is an opportunistic pathogen that easily adapts to shifting physicochemical conditions, showing a considerable capability of surviving and thriving in diverse environments, such as disinfectant solutions, making it a major nosocomial pathogen which is often isolated from urinary tract, respiratory tract, soft tissue, ocular, and septic infections [ 1 , 8 , 9 , 10 , 11 ]. The S. marcescens adaptation and survival in diverse environments can be traced to a number of genetic factors, including chromosomally-encoded porins and efflux systems that contribute to the resistance to several antibiotics [ 2 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Diminishing the Pathogenesis of the Food-Borne Pathogen Serratia marcescens by Low Doses of Sodium Citrate

Khayat

Elbaramawi

Nazeih

et al. 2023

Biology

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Protecting food from bacterial contamination is crucial for ensuring its safety and avoiding foodborne illness. Serratia marcescens is one of the food bacterial contaminants that can form biofilms and pigments that spoil the food product and could cause infections and illness to the consumer. Food preservation is essential to diminish such bacterial contaminants or at least reduce their pathogenesis; however, it should not affect food odor, taste, and consistency and must be safe. Sodium citrate is a well-known safe food additive and the current study aims to evaluate its anti-virulence and anti-biofilm activity at low concentrations against S. marcescens. The anti-virulence and antibiofilm activities of sodium citrate were evaluated phenotypically and genotypically. The results showed the significant effect of sodium citrate on decreasing the biofilm formation and other virulence factors, such as motility and the production of prodigiosin, protease, and hemolysins. This could be owed to its downregulating effect on the virulence-encoding genes. An in vivo investigation was conducted on mice and the histopathological examination of isolated tissues from the liver and kidney of mice confirmed the anti-virulence activity of sodium citrate. In addition, an in silico docking study was conducted to evaluate the sodium citrate binding ability to S. marcescens quorum sensing (QS) receptors that regulates its virulence. Sodium citrate showed a marked virtual ability to compete on QS proteins, which could explain sodium citrate’s anti-virulence effect. In conclusion, sodium citrate is a safe food additive and can be used at low concentrations to prevent contamination and biofilm formation by S. marcescens and other bacteria.

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Adherence of Serratia marcescens in the pathogenesis of urinary tract infections in diabetic mice

Cited by 12 publications

References 8 publications

Oral insulin delivery by self-assembled chitosan nanoparticles: In vitro and in vivo studies in diabetic animal model

Oral insulin delivery by self-assembled chitosan nanoparticles: In vitro and in vivo studies in diabetic animal model

Synergy in Polymicrobial Infections in a Mouse Model of Type 2 Diabetes

Diminishing the Pathogenesis of the Food-Borne Pathogen Serratia marcescens by Low Doses of Sodium Citrate

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