Adherence of Streptococcus mutans and Streptococcus sanguis to salivary components bound to glass

Abstract

Adherence of radiolabeled Streptococcus mutans and Streptococcus sanguis to saliva-treated glass surfaces was studied under conditions which minimized bacteria-glass interactions. Treatment of glass with an alkylsilane solution decreased nonspecific bacterial adherence and enhanced adsorption of radiolabeled salivary components to these surfaces. Addition of Triton X-100 to the bacterial suspensions also reduced nonspecific adherence to siliconized glass, but did not affect adherence to salivary components attached to siliconized glass. Calcium stimulated S. mutans adherence to saliva-free glass, but inhibited adherence to salivatreated glass. S. sanguis adherence to either saliva-free or saliva-treated glass was inhibited slightly at high calcium ion concentrations. Adherence of streptococci to saliva-treated glass exhibited saturation kinetics, and the numbers of binding sites on the experimental salivary pellicle and the affinity constants for bacteria-saliva attachment were determined. Preincubation of the streptococci with whole saliva decreased their capacity to adhere to saliva-treated glass, but not to saliva-free glass. Bacteria adherent to saliva-treated glass surfaces were readily desorbed by washing with saliva. The addition of homologous antisera, ammonium sulfate-precipitated immunoglobulins, or Fab fragments to the bacterial suspensions inhibited cell adherence to saliva-treated glass.

Streptococcus mutans strains recovered from caries‐active or caries‐free individuals differ in sensitivity to host antimicrobial peptides

“…A recent study also reported a positive correlation between the total protein and glycoprotein contents in saliva and saliva-promoted S. mutans adhesion to hydroxyapatite (Shimotoyodome et al, 2007). A number of salivary components, when adsorbed to oral surfaces, were reported to mediate molecular interactions with oral bacteria, including mucins (Stinson et al, 1982), a-amylase (Scannapieco et al, 1993(Scannapieco et al, , 1995, fibronectin (Dawson & Ellen, 1990) and proline-rich proteins (Gibbons & Hay, 1989;Russell & Mansson-Rahemtulla, 1989). Therefore, this pellicle-mediated bacterial adhesion could provide the basis for the robust formation of dental plaque populated with sufficient proportions of cariogens to increase the risk of dental caries.…”

An in vitro method to study the adherence of bacteria to saliva‐treated tooth enamel sections

“…A variety of ardficial and natural hard surfaces have been used for in vilro adherence studies, including slabs of bovine enamel (24), glass tubes (22), whale dentine (23), discs of compressed hydroxyapadte (4), saliva-treated glass (31) and Mylar strips (34). The most popular method to date appears to be the adherence assay described by Clark and co-workers in 1978 (3) which uses hydroxyapadte beads as a convenient, well-defined synthedc substrate.…”

In vitro adherence of oral streptococci to zirconia core and veneering glass‐ceramics

“…Subsequently, the various cells were harvested by centrifugation (2200 rpm, 19°C, 5 min; Hettich Rotixa P, Tuttlingen, G), washed twice with PBS and re‐suspended in the same buffer. Cell suspensions were subjected to low intensity ultrasonic energy to disperse streptococcal chains (Bandelin Sonorex Super RK 510 H, Bandelin electronic GmbH, Berlin, G),15 and the optical density of the suspensions was adjusted to 0.3 at 550 nm with a spectrophotometer (Genesys 10S; Thermo Spectronic, Rochester, NY), which corresponds to a microbial concentration of 3.65 × 10 8 microorganisms/mL 16…”