Adherence ofStaphylococcus epidermidis to human pharyngeal epithelial cells: Evidence for lipase-sensitive adhesin and glycoprotein receptor

Chugh, T.D.; Burns, Gary R.; H, Shuhaiber; Bishbishi, Eiman A.

doi:10.1007/bf01570834

Cited by 9 publications

(8 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, Nealon and Mattingly [14] showed that binding of group B streptococci to human embryonic and fetal epithelial cells is LTA-mediated, but their adherence to HeLa and primary amnion cell lines is protein-mediated. Therefore, we demonstrate clearly that LTA in the bacterial cell wall is the major adhesin, though not the sole one, of different biotypes and phage types of S. epidermidis [15,16]. It may contribute to their virulence by mediating bacterial attachment to specific receptors on cell membranes of various types of human cells.…”

Section: Discussionmentioning

confidence: 99%

Adhesion of <i>Staphylococcus epidermidis</i> to a Human Cardiac Cell Line Mediated by Lipoteichoic Acid

Chugh¹,

Burns

Bahr³

1999

Med Princ Pract

View full text Add to dashboard Cite

Objective: Staphylococcus epidermidis is a common cause of endocarditis. This study was carried out to examine the early steps of its pathogenesis, i.e. bacterial adhesion. Methods: Adherence of S. epidermidis to human, bovine and murine endothelial cell lines with and without pretreatment of cell lines with lipoteichoic acid (LTA) and pretreatment of bacteria with polyclonal anti-LTA antibodies, human albumin and lipase was carried out. Results: S. epidermidis binding to human continuous endocardial cell line was ∼3% of the inoculum. This was blocked with LTA (∼65%), polyclonal anti-LTA antibodies (∼50%), human albumin (∼60%) and lipase (∼60–70%). Conclusion: LTA is an important though not the sole adhesin for bacterium host cell receptor interaction in this experimental model.

show abstract

Section: Discussionmentioning

confidence: 99%

Adhesion of <i>Staphylococcus epidermidis</i> to a Human Cardiac Cell Line Mediated by Lipoteichoic Acid

Chugh¹,

Burns

Bahr³

1999

Med Princ Pract

View full text Add to dashboard Cite

show abstract

“…Neither strain produced slime, was encapsulated, or agglutinated human, rabbit, sheep, or guinea pig erythrocytes (7). The surface charge and hydrophobicity of both strains have been determined previously (8).…”

Section: Methodsmentioning

confidence: 99%

“…and phage type 63/138/245/336, while strain CH5 was of biotype 6706113 and was phage nontypeable. Strains CH2 and CH5 were identified as highly and poorly adherent strains (8), respectively, based on the number of bacteria adherent per pharyngeal epithelial cell in vitro (37.9 ± 4.3 versus 11.1 ± 2.0, respectively). Neither strain produced slime, was encapsulated, or agglutinated human, rabbit, sheep, or guinea pig erythrocytes (7).…”

Section: Methodsmentioning

confidence: 99%

“…The precise factors that lead to the adherence of S. epidermidis to host tissue and to artificial surfaces (prosthetic valves and surgical sutures) are not yet clear. We previously identified bacterial lipoteichoic acid (LTA) as the adhesin which mediates the attachment of S. epidermidis to human pharyngeal epithelial cells (8). In the present study, an attempt was made to identify the role of various factors which influence the adherence of S. epidermidis to the fibrin-platelet matrix.…”

mentioning

confidence: 91%

See 1 more Smart Citation

Adherence of Staphylococcus epidermidis to fibrin-platelet clots in vitro mediated by lipoteichoic acid

Chugh

Burns

et al. 1990

Infect Immun

View full text Add to dashboard Cite

The adherence of two strains of Staphylococcus epidermidis to human fibrin-platelet clots in vitro was investigated. Both strains were noncapsulated, nonhemagglutinating, and nonslime producers. Binding was not related to surface charge, carbohydrate profile, or hydrophobicity of the bacteria. Adherence was reduced fourto sixfold (P < 0.001) on pretreatment of bacteria with lipase, while neuraminidase, trypsin, phospholipase C, and sodium periodate did not alter their binding. Pretreatment of bacteria with substances known to bind lipoteichoic acid (LTA), such as human albumin and anti-LTA antibodies, also resulted in a fourfold (P < 0.001) reduction in adherence. Prior incubation of clots with free LTA, but not with deacylated LTA, produced a fourfold (P < 0.001) decrease in the adherence of homologous and heterologous strains of S. epidermidis. A similar reduction was also observed when LTAs derived from Staphylococcus aureus and Streptococcus pyogenes were used. These data provide evidence that the lipid moiety of LTA has a central role in the adherence of S. epidermidis to fibrin-platelet clots in vitro.

show abstract

“…In a previous report (58), we have shown that the two strains of S. aureus and S. epidermidis used in the present study share four antigenically similar, if not identical, surface peptides; thus, it is not unreasonable to speculate that these staphylococci, as well as S. saprophyticus, might share similar mucin-binding receptors. Chugh et al (11) recently reported that the S. epidermidis adhesin for human pharyngeal cells, prewashed to remove mucus, was sensitive to lipase but resistant to trypsin, which indicates that this adhesin is distinct from the mucin-binding receptor. From previous reports, it appears that lipoteichoic acid of S. saprophyticus is one of the adhesins mediating adhesion to uroepithelial cells (57) which is distinct from the trypsin-sensitive adhesin responsible for hemagglutinating sheep erythrocytes (30).…”

mentioning

confidence: 99%

Binding of staphylococci to mucus in vivo and in vitro

1989

View full text Add to dashboard Cite

The association of Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus saprophyticus with tissues of the upper respiratory tract were compared by using an in vivo ferret model. Ferrets were challenged intranasally with a 1-ml volume of radiolabeled staphylococci (3 mg [dry weight]), were allowed to clear the bacteria in vivo for 90 min, and were sacrificed. Tissues from the right nasal fossa were harvested and processed for radioassay or histology. Of the recoverable staphylococci, .96% was associated with mucus gel overlaying mucosa of the turbinates. A quantitative radioassay was developed to study the binding of labeled staphylococci to immobilized crude ferret nasal mucin (FM) and bovine submaxillary gland mucin (BM). Binding showed saturation kinetics and was blocked specifically by BM but not by human Tamm-Horsfall glycoprotein nor orosomucoid. Binding to both FM and BM was significantly inhibited (P c 0.01) when cocci were pretreated with trypsin but not when treated with I8-galactosidase or sodium metaperiodate (except for binding of S. saprophyticus to FM). These results suggest that mucin-binding receptors of the cocci may have protein components. The staphylococcus-binding receptors of both FM and BM appear to contain protein components, based on sensitivity to pretreatment with trypsin.

show abstract

Adherence ofStaphylococcus epidermidis to human pharyngeal epithelial cells: Evidence for lipase-sensitive adhesin and glycoprotein receptor

Cited by 9 publications

References 13 publications

Adhesion of <i>Staphylococcus epidermidis</i> to a Human Cardiac Cell Line Mediated by Lipoteichoic Acid

Adhesion of <i>Staphylococcus epidermidis</i> to a Human Cardiac Cell Line Mediated by Lipoteichoic Acid

Adherence of Staphylococcus epidermidis to fibrin-platelet clots in vitro mediated by lipoteichoic acid

Binding of staphylococci to mucus in vivo and in vitro

Contact Info

Product

Resources

About