Adherent/invasive Escherichia coli (AIEC) isolates from asymptomatic people: new E. coli ST131 O25:H4/H30-Rx virotypes

Barrios-Villa, Edwin; Cortés-Cortés, Gerardo; Lozano-Zaraín, Patricia; Arenas-Hernández, Margarita María de la Paz; Peña, Claudia F. Martínez de la; Martínez‐Laguna, Ygnacio; Torres, Carmén; Rocha-Gracia, Rosa del Carmen

doi:10.1186/s12941-018-0295-4

Cited by 16 publications

(14 citation statements)

References 82 publications

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“… 47 Barrios-Villa et al reported four ST131 E. coli strains isolated from healthy humans with a high adherence/invasive phenotype. 48 …”

Section: Discussionmentioning

confidence: 99%

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli from Healthy Children in the Central South China

Yang

et al. 2022

IDR

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Background Fecal carriage of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) and carbapenemase-producing E. coli (CP-EC) is well reported among hospitalized adults and children. However, there are few studies on the carriage prevalence and ESBL-EC and CP-EC genotypes among healthy children in China. Patients and Methods Stool samples were collected from 330 students in 2021 from three randomly selected primary schools in Changsha, China. ESBL-EC and CP-EC were screened using CHROMagar TM chromogenic plates. ESBL and carbapenemase production was confirmed using the double-disc synergy test and a modified carbapenem inactivation method, respectively. Antimicrobial susceptibility was tested using the broth microdilution method. Resistance determinants, virulence factors, and phylogenetic groups were determined by PCR and sequencing. Multi-locus sequence typing (MLST) was performed (seven housekeeping genes were amplified and sequenced) on the phylogenic group B2 E. coli to detect high-risk clonal strains such as ST131 E. coli . Then, ST131 E. coli were characterized based on ST131 clades, O-type, and fimH alleles. Results In total, 118 (35.8%) ESBL-EC and 3 (0.9%) CP-EC were isolated. bla CTX-M was the most common genotype (27.1%), identified in all ESBL-EC, except one, which carried bla SHV-12 . One isolate with mcr-1 was found amongst ESBL-EC, whereas all three CP-EC carried bla NDM-1 . The predominant sequence type (ST) clones in group B2 were ST131 and ST1193. The prevalence of ST131 E. coli was 9.9%, displaying serotypes O16 and O25b, fimH alleles 30, 41, and 89, and ST131 clades A and C1-M27. Conclusion In this study, high carriage rate of ESBL-EC was found among healthy children, and the dominant ESBL was CTX-M-14. In addition, high-risk clones (ST131 and ST1193) were also detected. This emphasizes the importance of monitoring ESBL-EC in community settings.

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“… 47 Barrios-Villa et al reported four ST131 E. coli strains isolated from healthy humans with a high adherence/invasive phenotype. 48 …”

Section: Discussionmentioning

confidence: 99%

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli from Healthy Children in the Central South China

Yang

et al. 2022

IDR

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“…18,32 Virotypes of all 101 ST131 isolates were determined as previously described. 18,33 Virotype C was most common, represented by 38/101 (37.6%) of…”

Section: Virotypesmentioning

confidence: 99%

Characterization of cefotaxime-resistant urinary Escherichia coli from primary care in South-West England 2017–18

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Gould

North

et al. 2019

Journal of Antimicrobial Chemotherapy

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Objectives Third-generation cephalosporin-resistant Escherichia coli from community-acquired urinary tract infections are increasingly reported worldwide. We sought to determine and characterize the mechanisms of cefotaxime resistance employed by urinary E. coli obtained from primary care, over 12 months, in Bristol and surrounding counties in South-West England. Methods Cefalexin-resistant E. coli isolates were identified from GP-referred urine samples using disc susceptibility testing. Cefotaxime resistance was determined by subsequent plating onto MIC breakpoint plates. β-Lactamase genes were detected by PCR. WGS was performed on 225 isolates and analyses were performed using the Center for Genomic Epidemiology platform. Patient information provided by the referring general practices was reviewed. Results Cefalexin-resistant E. coli (n=900) isolates were obtained from urines from 146 general practices. Following deduplication by patient approximately 69% (576/836) of isolates were cefotaxime resistant. WGS of 225 isolates identified that the most common cefotaxime-resistance mechanism was blaCTX-M carriage (185/225), followed by plasmid-mediated AmpCs (pAmpCs) (17/225), AmpC hyperproduction (13/225), ESBL blaSHV variants (6/225) or a combination of both blaCTX-M and pAmpC (4/225). Forty-four STs were identified, with ST131 representing 101/225 isolates, within which clade C2 was dominant (54/101). Ciprofloxacin resistance was observed in 128/225 (56.9%) of sequenced isolates, predominantly associated with fluoroquinolone-resistant clones ST131 and ST1193. Conclusions Most cefalexin-resistant E. coli isolates were cefotaxime resistant, predominantly caused by blaCTX-M carriage. The correlation between cefotaxime resistance and ciprofloxacin resistance was largely attributable to the high-risk pandemic clones ST131 and ST1193. Localized epidemiological data provide greater resolution than regional data and can be valuable for informing treatment choices in the primary care setting.

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“…18, 31 Virotypes of all 101 ST131 isolates were determined as previously described. 18, 32 Virotype C was most common, represented by 38/101 (37.6%) of ST131 isolates and predominantly associated with bla CTX-M-27 (28/38 isolates) across clades A (n=3), C1-M27 (n=22), C1-nM27 (n=4), and one isolate belonged to an unclassified clade. All bla CTX-M-27 -positive isolates belonged to virotype C, with the exception of one isolate for which a virotype could not be assigned.…”

Section: Resultsmentioning

confidence: 99%

Characterisation of cefotaxime-resistant urinary Escherichia coli from primary care in South-West England 2017-2018

Findlay

Gould

North

et al. 2019

Preprint

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AmpC hyperproduction (13/225; 5.8%), or a combination of both bla CTX-M and pAmpC 4 2 carriage (4/225; 1.8%). Forty-four sequence types (STs) were identified with ST131 4 3 representing 101/225 (45.0%) of sequenced isolates, within which the bla CTX-M-15 -positive 4 4 clade C2 was dominant (54/101; 53.5%). Ciprofloxacin-resistance (CIP-R) was observed in 4 5 128/225 (56.9%) of sequenced CTX-R isolates -predominantly associated with 4 6 fluoroquinolone-resistant clones ST131 and ST1193. 4 7 Conclusions: Most Ceph-R urinary E. colis were CTX-R, predominantly caused by bla CTX-M 4 8 carriage. There was a clear correlation between CTX-R and CIP-R, largely attributable to the 4 9

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Adherent/invasive Escherichia coli (AIEC) isolates from asymptomatic people: new E. coli ST131 O25:H4/H30-Rx virotypes

Cited by 16 publications

References 82 publications

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli from Healthy Children in the Central South China

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli from Healthy Children in the Central South China

Characterization of cefotaxime-resistant urinary Escherichia coli from primary care in South-West England 2017–18

Characterisation of cefotaxime-resistant urinary Escherichia coli from primary care in South-West England 2017-2018

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