Adhesins and invasins of pathogenic Escherichia coli

Bouguénec, Chantal Le

doi:10.1016/j.ijmm.2005.07.001

Cited by 77 publications

(57 citation statements)

References 57 publications

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“…As a common virulence mechanism, pathogenic bacteria adhere to or invade EC (22,26). To exclude the possibility that the tested commensal strains feature these virulence mechanisms, we determined whether E. coli K-12, Bifidobacterium adolescentis, Bacteroides vulgatus, Bacteroides distasonis, or Streptococcus salivarius adheres to or invades host EC.…”

Section: Resultsmentioning

confidence: 99%

Identification of Commensal Bacterial Strains That Modulate Yersinia enterocolitica and Dextran Sodium Sulfate-Induced Inflammatory Responses: Implications for the Development of Probiotics

et al. 2007

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An increasing body of evidence suggests that probiotic bacteria are effective in the treatment of enteric infections, although the molecular basis of this activity remains elusive. To identify putative probiotics, we tested commensal bacteria in terms of their toxicity, invasiveness, inhibition of Yersinia-induced inflammation in vitro and in vivo, and modulation of dextran sodium sulfate (DSS)-induced colitis in mice. The commensal bacteria Escherichia coli, Bifidobacterium adolescentis, Bacteroides vulgatus, Bacteroides distasonis, and Streptococcus salivarius were screened for adhesion to, invasion of, and toxicity for host epithelial cells (EC), and the strains were tested for their ability to inhibit Y. enterocolitica-induced NF-B activation. Additionally, B. adolescentis was administered to mice orally infected with Y. enterocolitica and to mice with mucosae impaired by DSS treatment. None of the commensal bacteria tested was toxic for or invaded the EC. B. adolescentis, B. distasonis, B. vulgatus, and S. salivarius inhibited the Y. enterocolitica-induced NF-B activation and interleukin-8 production in EC. In line with these findings, B. adolescentis-fed mice had significantly lower results for mean pathogen burden in the visceral organs, intestinal tumor necrosis factor alpha mRNA expression, and loss of body weight upon oral infection with Y. enterocolitica. In addition, the administration of B. adolescentis decelerated inflammation upon DSS treatment in mice. We suggest that our approach might help to identify new probiotics to be used for the treatment of inflammatory and infectious gastrointestinal disorders.

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Section: Resultsmentioning

confidence: 99%

Identification of Commensal Bacterial Strains That Modulate Yersinia enterocolitica and Dextran Sodium Sulfate-Induced Inflammatory Responses: Implications for the Development of Probiotics

et al. 2007

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“…It is important to note that it has been established that the cell entry of Afa/Dr DAF/CEACAMs DAEC into epithelial cells is triggered by their major adhesin subunits E (538,539). The role of the D subunits of Afa/Dr DAF/CEACAMs DAEC, known as invasins (540,541), in the entry into epithelial cells remains controversial (542)(543)(544)(545)(546)(547).…”

Section: Cell Interaction Cell Entry and Intracellular Lifestylementioning

confidence: 99%

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Moal

Servin

2013

Microbiol Mol Biol Rev

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SUMMARY Hosts are protected from attack by potentially harmful enteric microorganisms, viruses, and parasites by the polarized fully differentiated epithelial cells that make up the epithelium, providing a physical and functional barrier. Enterovirulent bacteria interact with the epithelial polarized cells lining the intestinal barrier, and some invade the cells. A better understanding of the cross talk between enterovirulent bacteria and the polarized intestinal cells has resulted in the identification of essential enterovirulent bacterial structures and virulence gene products playing pivotal roles in pathogenesis. Cultured animal cell lines and cultured human nonintestinal, undifferentiated epithelial cells have been extensively used for understanding the mechanisms by which some human enterovirulent bacteria induce intestinal disorders. Human colon carcinoma cell lines which are able to express in culture the functional and structural characteristics of mature enterocytes and goblet cells have been established, mimicking structurally and functionally an intestinal epithelial barrier. Moreover, Caco-2-derived M-like cells have been established, mimicking the bacterial capture property of M cells of Peyer's patches. This review intends to analyze the cellular and molecular mechanisms of pathogenesis of human enterovirulent bacteria observed in infected cultured human colon carcinoma enterocyte-like HT-29 subpopulations, enterocyte-like Caco-2 and clone cells, the colonic T84 cell line, HT-29 mucus-secreting cell subpopulations, and Caco-2-derived M-like cells, including cell association, cell entry, intracellular lifestyle, structural lesions at the brush border, functional lesions in enterocytes and goblet cells, functional and structural lesions at the junctional domain, and host cellular defense responses.

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“…It is noteworthy that physical trapping of bacteria on intermicrovillous cleft is different from pathogenic adherence that requires specific machinery such as pili, fimbriae, adhesin, intimin etc. 63 We cannot rule out changes in virulent properties (e.g., adherent ability) of enteric bacteria which may also contribute to and are not mutually exclusive from our findings in regards to abnormal hostmicrobe interaction.…”

Section: Faulty Host or Bug Or Both?mentioning

confidence: 62%

Commensal bacterial internalization by epithelial cells: An alternative portal for gut leakiness

2015

Tissue Barriers

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Adhesins and invasins of pathogenic Escherichia coli

Cited by 77 publications

References 57 publications

Identification of Commensal Bacterial Strains That Modulate Yersinia enterocolitica and Dextran Sodium Sulfate-Induced Inflammatory Responses: Implications for the Development of Probiotics

Identification of Commensal Bacterial Strains That Modulate Yersinia enterocolitica and Dextran Sodium Sulfate-Induced Inflammatory Responses: Implications for the Development of Probiotics

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Commensal bacterial internalization by epithelial cells: An alternative portal for gut leakiness

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