Adhesion: A tactic in the survival strategy of a marine vibrio during starvation

Dawson, M. Patricia; Humphrey, Beverley A.; Marshall, K. C.

doi:10.1007/bf01566971

Cited by 225 publications

(155 citation statements)

References 11 publications

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“…After cell lysis, the bacteria rapidly gain access to those essential nutrients to survive and propagate before being expelled from the host. V. alginolyticus is the most prevalent species of Vibrio in the marine environment and usually lives in oligotrophic conditions (Dawson et al, 1981;Xie et al, 2005). As an opportunistic pathogen, it may utilize the above mechanism to quickly establish environmental niches in a variety of host tissues and to penetrate host barrier defences.…”

Section: Discussionmentioning

confidence: 99%

The type III secretion system of Vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells

Zhao

Chen

et al. 2010

Microbiology

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Vibrio alginolyticus is a Gram-negative bacterium and has been recognized as an opportunistic pathogen in humans as well as marine animals. However, the virulence mechanisms for this species of Vibrio have not been elucidated. This study characterized multiple mechanisms that induce cell death in fish cells upon infection with a V. alginolyticus strain, ZJO. The bacterium required its type III secretion system (T3SS) to cause rapid death of infected fish cells. Dying cells exhibited some features of apoptotic cells, such as membrane blebbing, nuclear condensation and DNA fragmentation. Further studies showed that caspase-3 was activated by the T3SS of the ZJO strain, confirming that infection with V. alginolyticus rapidly induces T3SS-dependent apoptosis in fish cells. Infection with the ZJO strain also led to membrane pore formation and release of cellular contents from infected fish cells, as evidenced by lactate dehydrogenase release and the uptake of a membrane-impermeable dye. Importantly, inhibition of apoptosis did not prevent ZJO-infected cells from releasing cellular contents and did not block cell rounding. Taken together, these data demonstrate that infection with V. alginolyticus may promote at least three different T3SS-dependent events, which lead to the death of fish cells. This study provides an important insight into the mechanism used by Vibrio species to cause host-cell death.

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Section: Discussionmentioning

confidence: 99%

The type III secretion system of Vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells

Zhao

Chen

et al. 2010

Microbiology

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“…Several studies have reported that microorganisms exhibit active adhesion/detachment processes that may be a response to local nutrient availability [121,146,160], survival mechanisms [146,152,190], and/or growth ( [60], 1986; [99,179]). No generally accepted quantitative treatment of dynamic biologically mediated adhesion/detachment processes exists.…”

Section: Active Adhesion/detachmentmentioning

confidence: 99%

Processes in microbial transport in the natural subsurface

Ginn

Wood

Nelson

et al. 2002

Advances in Water Resources

269

131

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“…The metabolic realignments may be important not only in securing the viability of the cells during starvation, but also in making the cells more efficient scavengers of scarce nutrients. Some of the physiological changes demonstrated by members of the family Vibrionaceae in response to starvation indicate the importance of recovery-related and substrate-scavenging functions : starved cells have an increased high-affinity uptake of amino acids (MArdCn et al, 1987), an increased uptake capacity for glucose and mannose (Albertson et al, 1990 a), a continuing synthesis of exoproteases (Albertson et al, 1990b) and an increased adhesion to hydrophobic surfaces (Dawson et al, 1981 ;Kjelleberg et al,, 1982). However, the environmental triggers and the kinetics of the macromolecular changes involved in recovery are poorly understood with respect to copiotrophic, non-differentiating marine bacteria.…”

Section: H Albertson T Nystrom and S Kjellebergmentioning

confidence: 99%

Macromolecular synthesis during recovery of the marine Vibrio sp. S14 from starvation

Albertson

Nyström

Kjelleberg

1990

Journal of General Microbiology

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The recovery of Vibrio sp. S14 cells from energy-and nutrient-starvation was monitored after the addition of glucose minimal medium. Upshift experiments were done throughout a starvation period of 200 h to determine whether cells were more responsive to nutrient addition at the onset of starvation, or if the previously described programme of starvation-induced cellular reorganization had to be completed before cells could become committed to recovery following nutritional upshifts. The kinetics of macromolecular synthesis (RNA, protein and DNA), the rate of respiration and changes in median cell volume in response to nutritional upshifts at different times of starvation were examined. The relative rates of RNA and protein synthesis increased immediately upon addition of glucose minimal medium; the increase in protein synthesis was not dependent on a parallel increase in RNA synthesis, indicating that the starved cells may have an excess of protein synthesizing machinery, including stable RNA and functional ribosom?. The subsequent increase in the rate of DNA replication was initiated approximately 60min before the first apparent cell division at approximately one doubling of the theoretical minimal cell volume ( Vu). Two-dimensional gel electrophoresis was used to demonstrate the fate of starvationspecific proteins during the upshift, and also the synthesis of recovery-induced proteins that were not found in starving cells. Most starvation-inducible proteins were repressed immediately at the onset of the nutritional upshift, while 11 of the 21 recovery-induced proteins identified were expressed exclusively during the maturation phase and were subsequently repressed at the onset of regrowth. The possible role of such maturation-specific proteins in the rapid formation of a reproductive cell committed to growth and division is discussed.

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Adhesion: A tactic in the survival strategy of a marine vibrio during starvation

Cited by 225 publications

References 11 publications

The type III secretion system of Vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells

The type III secretion system of Vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells

Processes in microbial transport in the natural subsurface

Macromolecular synthesis during recovery of the marine Vibrio sp. S14 from starvation

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