Adhesion and biofilm formation of Candida parapsilosis isolated from vaginal secretions to copper intrauterine devices

Borges, Kátia Regina Assunção; Pimentel, Igor Vinícius; Lucena, Lidiane Cristina Lima dos Santos; Silva, Marcos Antônio Custódio Neto da; Monteiro, Sílvio Gomes; Monteiro, Cristina de Andrade; Nascimento, Maria do Desterro Soares Brandão

doi:10.1590/s1678-9946201860059

Cited by 17 publications

(14 citation statements)

References 17 publications

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“…C. parapsilosis is frequently found as a cause of pathologies due to biofilm formation on medical devices in long-term hospitalized patients suffering from endocarditis, peritonitis, arthritis, or general sepsis [ 22 , 38 , 39 , 40 ]. We hypothesized that the capacity to form biofilm might be related to the origin of the clinical specimen, that is, infections at different body sites or fungus growing on medical devices such as indwelling catheters.…”

Section: Discussionmentioning

confidence: 99%

Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates

Gómez-Molero

De-la-Pinta

Fernández-Pereira

et al. 2021

JoF

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Candida parapsilosis is a frequent cause of fungal bloodstream infections, especially in critically ill neonates or immunocompromised patients. Due to the formation of biofilms, the use of indwelling catheters and other medical devices increases the risk of infection and complicates treatment, as cells embedded in biofilms display reduced drug susceptibility. Therefore, biofilm formation may be a significant clinical parameter, guiding downstream therapeutic choices. Here, we phenotypically characterized 120 selected isolates out of a prospective collection of 215 clinical C. parapsilosis isolates, determining biofilm formation, major emerging colony morphotype, and antifungal drug susceptibility of the isolates and their biofilms. In our isolate set, increased biofilm formation capacity was independent of body site of isolation and not predictable using standard or modified European Committee on Antimicrobial Susceptibility Testing (EUCAST) drug susceptibility testing protocols. In contrast, biofilm formation was strongly correlated with the appearance of non-smooth colony morphotypes and invasiveness into agar plates. Our data suggest that the observation of non-smooth colony morphotypes in cultures of C. parapsilosis may help as an indicator to consider the initiation of anti-biofilm-active therapy, such as the switch from azole- to echinocandin- or polyene-based strategies, especially in case of infections by potent biofilm-forming strains.

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Section: Discussionmentioning

confidence: 99%

Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates

Gómez-Molero

De-la-Pinta

Fernández-Pereira

et al. 2021

JoF

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Section: Methodsmentioning

confidence: 99%

“…Five centimeter-diameter fragments of dental material (metal or acrylic resin) were generated as described by Silva et al [36] and Borges et al [37] with modifications. These fragments were cultivated in 100 μl of saline containing a 1×10 4 cell/mL suspension of C. albicans, C. parapsilosis, or C. tropicalis and kept in a BOD greenhouse for 3, 6, or 12 h for adherence assays or for 24 and 48 h for biofilm formation assays.…”

Section: Adherence and Biofilm Formation On Abiotic And Acrylic Resinmentioning

confidence: 99%

Highly efficient antibiofilm and antifungal activity of green propolis against Candida species in dentistry materials

et al. 2020

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This study evaluated the effect of green propolis extract on the adhesion and biofilm formation of Candida species in dentistry materials. Phytochemical analysis of green propolis extract was performed by high-performance liquid chromatography. Adhesion was quantified by counting the number of yeast cells adherent to dental material fragments in a Neubauer chamber. Biofilm formation was determined by counting colony-forming units recovered from dental material fragments. The intensity of biofilm adhesion was classified as negative, weak, moderate, strong, or very strong. Fifteen compounds, mainly flavonoids, were identified in green propolis extract. All strains adhered to and formed biofilms on the surfaces of the orthodontic materials studied. On steel and resin, yeast cell adhesion intensities were weak at all incubation times, except for those of Candida parapsilosis and C. tropicalis, which were moderate at 12 h. At 24 and 48 h, C. albicans formed biofilms on steel with moderate adhesion affinities; at 24 and 48 h, C. parapsilosis formed biofilms with very strong affinities. C. tropicalis formed biofilms with strong and very strong affinities at 24 and 48 h, respectively. On resin, all species displayed strong affinity for biofilm formation at 24 and 48 h, except for C. tropicalis, which displayed very strong affinity at only 48 h. Green propolis extract displayed antifungal activity and inhibited both adhesion and biofilm formation at 2.5 μg/mL. This study reinforces the idea that green propolis has antifungal activity and interferes with the virulence of Candida species.

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“…5 cm-sized fragments of dental material (metal and acrylic resin) were made and used in this study as described by Silva et al (2010) (26) and Borges et al (2018) (27) with modifications. The fragments were cultivated in saline with 100μl of a 1×10 4 cel/mL suspension of C. albicans , C. parapsilosis and C. tropicalis and they were kept in a BOD greenhouse at times of 3, 6 and 12hs for adherence and 24 and 48hs for biofilm in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Highly Efficient Antibiofilm and Antifungal Activity of Green Propolis againstCandidaspecies in dentistry material

Bezerra

Borges

Alves

et al. 2020

Preprint

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31Background: This study evaluated the influence of green propolis' extract on the 32 adhesion and biofilm formation of Candida species on dentistry material. Methods: 33 Phytochemical analysis of green propolis' extract was performed by High Performance 34 Liquid Chromatography. Adhesion was quantified in a Neubauer chamber, counting the 35 number of yeast cells adhered to the fragments; Biofilm formation was determined by 36 counting the number of colony forming units (CFU). The intensity of biofilm formation 37 adhesion was classified as negative, weak, moderate, strong and very strong. Fifteen 38 compounds were identified in green propolis extract, mainly flavonoids. Results: All 39 strains were able to adhere and form biofilm on the surface of the orthodontic materials 40 studied. In steel and resin, the adhesion intensity of the yeast cells was weak at all 41 incubation times, except for C. parapsilosis and C. tropicalis which at 12hs showed 42 moderate intensity. Regarding biofilm formation (24 and 48 hours), it was observed in 43 the steel that C. albicans had moderate intensity at 24 and 48 hours; C. parapsilosis at 44 24 and 48 hours had very strong intensity; C. tropicalis at 24 hours had strong intensity 45 and at 48 hours very strong. While in the resin, all species at 24 and 48 hours had strong 46 intensity, except for C. tropicalis which at 48 hours had very strong intensity. Green 47 propolis extract showed antifungal activity and was able to inhibit both adhesion and 48 biofilm formation at 2.5 µg/mL. Conclusions: This study reinforces the idea that green 49 propolis has antifungal activity and interferes with virulence factors of Candida species. 50 Keywords: Green Propolis; Candida sp, Biofilm; Dentistry material. 51 52 53 54 55 56 57 58 3 59 BACKGROUND 60In recent years the use of orthodontic materials has increased for aesthetic, surgical 61 and biofunctional purposes. Polymers, ceramics, composites, resin, steels and their 62 alloys are used in the manufacture of dental prostheses, screws and orthodontic 63 appliances and when implanted in the oral cavity they are exposed to colonization and 64 biofilm formation by microorganisms that live in the oral cavity. Alongside with the pH 65 and saliva, these devices are targets of biofilm formation especially produced by 66 Candida spp. (1). 67 A combination of factors contribute to Candida sp biofilm formation, salivary 68 flow, low pH, poor oral hygiene and the type of orthodontic material contribute to 69 biofilm colonization and formation (2). During colonization and biofilm formation, oral 70 microbiota secrete enzymes and exopolysaccharides to colonize a surface, thus the 71 biofilm constitutes as a film of organic components that are absorbed from saliva 72 forming an extracellular polymeric matrix and thus the multicellular community 73 (bacteria or fungus) is incorporated into the extracellular matrix (ECM) (1-3). 74The formation of biofilm in orthodontic materials raises concern as, when 75 installed, increases the risk of infection...

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Adhesion and biofilm formation of Candida parapsilosis isolated from vaginal secretions to copper intrauterine devices

Cited by 17 publications

References 17 publications

Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates

Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates

Highly efficient antibiofilm and antifungal activity of green propolis against Candida species in dentistry materials

Highly Efficient Antibiofilm and Antifungal Activity of Green Propolis againstCandidaspecies in dentistry material

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