Adhesion and ingestion activities of fish phagocytes induced by bacterium Aeromonas salmonicida can be distinguished and directly measured from highly diluted whole blood of fish

Nikoskelainen, Sami; Verho, Sanna; Airas, Kalervo; Lilius, Esa‐Matti

doi:10.1016/j.dci.2004.10.006

Cited by 50 publications

(31 citation statements)

References 59 publications

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“…Unlike the blood and head kidney phagocytes of rainbow trout [7], blood phagocytes of cod generated RB activity even in the absence of bacteria. This observation suggests that the blood phagocytes of cod are more adhesive on the plastic surfaces than trout phagocytes and that the cod phagocytes are directly activated by the wall of the microplates.…”

Section: Discussionmentioning

confidence: 93%

“…To evaluate the starting point of ingestion, duplicate samples were combined with cytochalasin D (final concentration of 3 mM), a known agent to inhibit cell movements and phagocytosis. From these measurements it was estimated that, similarly as in [7], the CL response generated before 15 min was a response to adhesion (data not shown). Gelatin markedly decreased the magnitude of the second peak of ingestion (Fig.…”

Section: The Effects Of Serum and Gelatin Concentration On The Respirmentioning

confidence: 99%

“…As with the earlier RB assays of trout the RB activity of cod blood phagocytes was induced in parallel with opsonised (OB) and non-opsonised (NOB) As MT004 cells. Additionally, 0.1% of gelatin was included in all measurements to decrease the first peak of expected biphasic response (adhesion and ingestion responses) [7]. At a final blood concentration of 2.0 ml ml ÿ1 or less, practically no RB activity was detected when cod phagocytes were stimulated with NOB.…”

Section: The Effect Of Blood Dilutionmentioning

confidence: 99%

“…Luminol-amplified chemiluminescence (CL) is widely used for the sensitive detection of reactive oxygen species (ROS) production in phagocytic cells. Recent studies have shown that the ingestion of bacteria can be measured directly from highly diluted blood of trout by the CL emission of blood phagocytes [7]. Additionally, the enhancing effect of specific antibodies, if present, on the ingestion of bacteria can also be determined by the CL measurements [8].…”

Section: Introductionmentioning

confidence: 99%

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Respiratory burst activity of Atlantic cod (Gadus morhua L.) blood phagocytes differs markedly from that of rainbow trout

Nikoskelainen

Kjellsen

Lilius

et al. 2006

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 93%

Section: The Effects Of Serum and Gelatin Concentration On The Respirmentioning

confidence: 99%

Section: The Effect Of Blood Dilutionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Respiratory burst activity of Atlantic cod (Gadus morhua L.) blood phagocytes differs markedly from that of rainbow trout

Nikoskelainen

Kjellsen

Lilius

et al. 2006

Fish & Shellfish Immunology

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“…Induction of the respiratory burst (RB) activity in blood leucocytes was measured directly from heparinised blood following the method described by Nikoskelainen et al (2005) with some modifications. Briefly, 275 ll of diluted blood (1:200) in gHBSS-hep-luminol (Hanks' Balanced Salt Solution containing 0.1% gelatin, 5 U/ml of heparin, 100 I.U./ml of penicillin/streptomycin, 5 lg/ml of oxytetracycline, 0.19 mM luminol, pH 7.4) was dispensed in triplicate wells of a white flat-bottomed 96-well microplate.…”

Section: Chemiluminescencementioning

confidence: 99%

Effects of additive iron on growth, tissue distribution, haematology and immunology of gilthead sea bream, Sparus aurata

et al. 2010

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The comparative effects of iron-supplemented levels on growth, tissue distribution, haematology and immunology of gilthead sea bream, Sparus aurata (2 g) were investigated, using four organic (50, 100, 200, 300 mg ORG/kg diet) and one inorganic iron source (200 INOR mg/kg diet). Fish were treated for 12 weeks with the experimental diets and maintained at a water temperature of 19-22°C. Growth (final weight and specific growth rate), tissue distribution (spleen, liver and muscle), haematological parameters (red blood cells, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration) and non-specific immune indexes (respiratory burst activity and antibacterial activity of serum) were analysed. No significant differences were found in growth and iron tissue distribution among the tested groups. Red blood cell counting was statistically higher in fish given 50 ORG, 100 ORG, 200 ORG and 200 INOR feeds. However, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration were not significantly affected by increasing dietary iron. Fish receiving the 100 ORG diet had the best performance with respect to the respiratory burst activity and significantly higher values for antibacterial activity of serum were obtained in fish fed with the 300 ORG diet. The present findings provided no clear evidence of the optimum iron concentration. However, there was adequate indication that iron supplementation enhanced the performance of gilthead sea bream, mainly from a haematological and immunological point of view.

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Tissue-specific responses of oxidative stress biomarkers and antioxidant defenses in rainbow trout Oncorhynchus mykiss during a vaccination against furunculosis

Tkachenko¹,

Kurhaluk²,

Grudniewska

et al. 2014

Fish Physiol Biochem

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The present study was conducted to evaluate the effects of vaccination against furunculosis on responses of oxidative stress and antioxidant defenses in rainbow trout Oncorhynchus mykiss muscle, gills, liver, and brain tissues. The oxidative stress markers (malondialdehyde and carbonyl derivatives of protein oxidative destruction levels), antioxidant defenses (superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase), and total antioxidant capacity in different tissues of rainbow trout were measured. Our data showed that exposure of trout to vaccine against furunculosis produced changes (either increase or decrease) in oxidative stress and antioxidant enzymes responses, and these responses showed marked organ differences, associated with tissue patterns. Our study demonstrated that vaccinated trout showed alteration in antioxidant defenses and oxidative stress responses, with higher severity in the liver, compared with other tissues. Our data also suggest that vaccination against furunculosis induced lipid peroxidation in gill and liver tissues. However, muscle and brain tissue are capable of restoring its pro- and antioxidant balance after vaccination.

show abstract

Adhesion and ingestion activities of fish phagocytes induced by bacterium Aeromonas salmonicida can be distinguished and directly measured from highly diluted whole blood of fish

Cited by 50 publications

References 59 publications

Respiratory burst activity of Atlantic cod (Gadus morhua L.) blood phagocytes differs markedly from that of rainbow trout

Respiratory burst activity of Atlantic cod (Gadus morhua L.) blood phagocytes differs markedly from that of rainbow trout

Effects of additive iron on growth, tissue distribution, haematology and immunology of gilthead sea bream, Sparus aurata

Tissue-specific responses of oxidative stress biomarkers and antioxidant defenses in rainbow trout Oncorhynchus mykiss during a vaccination against furunculosis

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