Adhesion and migration of cells responding to microtopography

Estévez, Maruxa; Martínez, Elena; Yarwood, Stephen J.; Dalby, Matthew J.; Samitier, J.

doi:10.1002/jbm.a.35293

Cited by 32 publications

(25 citation statements)

References 38 publications

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“…[45][46][47] Therefore, FAK phosphorylation may serve as a biomarker for cell-material interactions. [48][49][50] In our study, rough Ti (grade 3) inhibited FAK phosphorylation of HCF at 1.5 months in the absence of TGFb1. According to proposed FAK signaling pathways, 51 FAK mediates TGFb1 signaling, which in turn activates a-SMA expression and promotes collagen deposition by myofibroblasts.…”

Section: Discussionmentioning

confidence: 93%

“…However, the expression of a-SMA in grade 3 Ti was significantly reduced, suggesting that a-SMA expression via FAK phosphorylation is inhibited in the presence of rough topographic cues. Since cells can form various adhesion structures even without FAK, 50 alternative signaling routes, such as the integrin-Src linkage, 45 may be more critical in the signaling evoked by Ti surface topography. Therefore, the role of TGFb1 signaling in corneal fibroblast transformation is yet to be determined in the context of surface topographic stimulation.…”

Section: Discussionmentioning

confidence: 99%

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The Role of Titanium Surface Microtopography on Adhesion, Proliferation, Transformation, and Matrix Deposition of Corneal Cells

Zhou

Lei

Chodosh

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Titanium (Ti) is an excellent implantable biomaterial that can be further enhanced by surface topography optimization. Despite numerous data from orthopedics and dentistry, the effect of Ti surface topography on ocular cells is still poorly understood. In light of the recent adaptation of Ti in the Boston Keratoprosthesis artificial cornea, we attempted to perform an extended evaluation of the effect of Ti surface topography on corneal cell adhesion, proliferation, cytotoxicity, transformation, and matrix deposition.METHODS. Different surface topographies were generated on medical grade Ti-6Al-4V-ELI (extra-low interstitial), with linearly increased roughness (polished to grit blasted). Biological response was evaluated in vitro using human corneal limbal epithelial (HCLE) cells, stromal fibroblasts (HCF), and endothelial cells (HCEnC). RESULTS.None of the Ti surface topographies caused cytotoxicity to any of the three corneal cell types. However, rough Ti surface inhibited HCLE and HCF cell adhesion and proliferation, while HCEnC proliferation was unaffected. Long-term experiments with HCF revealed that rough Ti surface with R a (the arithmetic average of the profile height from the mean line) ‡ 1.15 lm suppressed HCF focal adhesion kinase phosphorylation, changed fibroblast morphology, and caused less aligned and reduced deposition of collagen matrix as compared to smooth Ti (R a 0.08 lm). In the presence of transforming growth factor b1 (TGFb1) stimulation, rough Ti inhibited alpha-smooth muscle actin (a-SMA) expression and collagen deposition, leading to decreased myofibroblast transformation and disorganization of the collagen fibrils as compared to smooth Ti.CONCLUSIONS. This study suggests that Ti surface topography regulates corneal cell behavior in a tissue-dependent manner that varies across the corneal strata. Contrary to the accepted paradigm, smooth surface topography can enhance cell adhesion and proliferation and increase matrix deposition by corneal cells.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

The Role of Titanium Surface Microtopography on Adhesion, Proliferation, Transformation, and Matrix Deposition of Corneal Cells

Zhou

Lei

Chodosh

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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“…Amongst these improvements, controlled nanopatterning of the biomaterial surface has been shown to directly influence human stem cell proliferation and differentiation, giving it an important advantage compared to uncontrolled topographies and planar surfaces 5,6 . Despite these positive effects, the effect of biomaterial nanopatterning on bacterial surface colonisation remains unknown 7 .…”

Section: Introductionmentioning

confidence: 99%

Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells

et al. 2016

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Abstract:Despite the well-known beneficial effects of biomaterial nanopatterning on host tissue integration, the influence of controlled nanoscale topography on bacterial colonisation and infection remains unknown. Therefore, the aim of the present study was to determine the nanoscale effect of surface nanopatterning on biomaterial colonisation by S. aureus, utilising AFM nanomechanics and single-cell force spectroscopy (SCFS). Nanoindentation of S. aureus bound to planar (PL) and nanopatterned (SQ) polycarbonate (PC) surfaces suggested two distinct areas of mechanical properties, consistent with a central bacterial cell surrounded by a capsullar component. Nevertheless, no differences in elastic moduli were found between bacteria bound to PL and SQ, suggesting a minor role of nanopatterning in bacterial cell elasticity. Furthermore, SCFS demonstrated increased adhesion forces and work between S. aureus and SQ surfaces at 0s and 1s contact times. Although WLC modelling showed similarities in contour lengths for attachment to both surfaces, Poisson analysis suggests increased short-range forces for the S. aureus-SQ interactions. In the case of S. aureus-PL, long-range forces were found to not only be dominant but also repulsive in nature, which may help explain the reduced adhesion forces observed during AFM probing. In conclusion, although surface nanopatterning does not significantly influence the elasticity of attached bacterial cells, it was found to promote the early-adhesion of S. aureus cells to the biomaterial surface.

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“…The influence of nanoscale and microscale surface features on cell response provided motivation for this work. Cells sense nanoscale and microscale surface features, which influences cell adhesion and promotes differentiation and migration [39,40]. Etched 3DP PCL scaffolds had microscale features and increased surface roughness that induced osteogenic differentiation of human bone marrow stromal cells, while the control 3DP PCL scaffolds did not [9].…”

Section: Evaluation Of Cell Response To Ff Scaffoldsmentioning

confidence: 99%

Freeze-FRESH: A 3D Printing Technique to Produce Biomaterial Scaffolds with Hierarchical Porosity

Wang

Florczyk

2020

Materials

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Tissues are organized in hierarchical structures comprised of nanoscale, microscale, and macroscale features. Incorporating hierarchical structures into biomaterial scaffolds may enable better resemblance of native tissue structures and improve cell interaction, but it is challenging to produce such scaffolds using a single conventional scaffold production technique. We developed the Freeze-FRESH (FF) technique that combines FRESH 3D printing (3DP) and freeze-casting to produce 3D printed scaffolds with microscale pores in the struts. FF scaffolds were produced by extrusion 3DP using a support bath at room temperature, followed by freezing and lyophilization, then the FF scaffolds were recovered from the bath and crosslinked. The FF scaffolds had a hierarchical pore structure from the combination of microscale pores throughout the scaffold struts and macroscale pores in the printed design, while control scaffolds had only macroscale pores. FF scaffolds frozen at −20 °C and −80 °C had similar pore sizes, due to freezing in the support bath. The −20 °C and −80 °C FF scaffolds had porous struts with 63.55% ± 2.59% and 56.72% ± 13.17% strut porosity, respectively, while control scaffolds had a strut porosity of 3.15% ± 2.20%. The −20 °C and −80 °C FF scaffolds were softer than control scaffolds: they had pore wall stiffness of 0.17 ± 0.06 MPa and 0.23 ± 0.05 MPa, respectively, compared to 1.31 ± 0.39 MPa for the control. The FF scaffolds had increased resilience in bending compared with control. FF scaffolds supported MDA-MB-231 cell growth and had significantly greater cell numbers than control scaffolds. Cells formed clusters on the porous struts of FF scaffolds and had similar morphologies as the freeze cast scaffolds. The FF technique successfully introduced microscale porosity into the 3DP scaffold struts to produce hierarchical pore structures that enhanced MDA-MB-231 growth.

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Adhesion and migration of cells responding to microtopography

Cited by 32 publications

References 38 publications

The Role of Titanium Surface Microtopography on Adhesion, Proliferation, Transformation, and Matrix Deposition of Corneal Cells

The Role of Titanium Surface Microtopography on Adhesion, Proliferation, Transformation, and Matrix Deposition of Corneal Cells

Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells

Freeze-FRESH: A 3D Printing Technique to Produce Biomaterial Scaffolds with Hierarchical Porosity

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