Adhesion and proliferation of cells on new polymers modified biomaterials

Lakard, Sophie; Herlem, Guillaume; Propper, Alain; Kastner, Anders; Michel, Germaine; Vallès-Villarreal, N; Gharbi, Tijani; Fahys, Bernard

doi:10.1016/j.bioelechem.2003.09.009

Cited by 85 publications

(62 citation statements)

References 41 publications

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“…Furuya et al 14) reported that approximately 440 ng/cm 2 of fibronectin absorbed on titanium (titanium sensor of QCM apparatus). The absorbed amount of fibronectin detected in the present study (75.6 ng/cm 2 ) was approximately 17 % of the absorbed amount reported by Furuya et al Because many factors, including electrostatic interaction, hydrogen bonding and hydrophobic interaction [15][16][17][18] , influence the adsorption of proteins onto biomaterials, the detailed mechanism of low protein adsorption is still unclear. We presumed that low protein adsorption affected poor dermal fibroblast cell attachment, resulting in cell spheroid formation.…”

Section: Discussioncontrasting

confidence: 50%

Biological and Chemical Assessment of DNA/Chitosan Complex Film

Inoue¹,

Kawaguchi²,

Masui³

et al. 2014

J. Hard Tissue Biology.

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Transparent DNA/chitosan complex film was prepared from DNA/chitosan complex powder via hydrothermal hot pressing. In this study, we investigated the binding of daunorubicin hydrochloride (DH), proteins adsorption (fibronectin and albumin), mineral deposition, and rat soft-tissue response to the DNA/ chitosan complex film to determine whether this film has potential for use in membranes for bone tissue engineering. The binding ratio of DH to DNA/chitosan complex film was very close to the excision number of 3.7 for the binding of DH by native DNA. This indicated that DNA in the complex did not denature as a result of hot pressing. Apatite was formed on the surface of the film after immersion in simulated body fluid. However, quartz crystal microbalance analysis showed that protein absorption to the DNA/chitosan complex was very low. It was found that this film has an affinity for minerals rather than proteins. Although the film remained in an almost unchanged configuration at 21 days after subcutaneous implantation, the response of rat soft tissues to the film was mild. These results suggest that DNA/chitosan complex film has potential for use as a membrane in bone tissue engineering.

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Section: Discussioncontrasting

confidence: 50%

Biological and Chemical Assessment of DNA/Chitosan Complex Film

Inoue¹,

Kawaguchi²,

Masui³

et al. 2014

J. Hard Tissue Biology.

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“…The cells (mice fibroblast cells NIH-3T3) have a similar morphology to the homogeneously distributed positive control cells, with elongated appearance and numerous cytoplasmic extensions and cell-to-cell interactions [36,37]. Cell proliferation was not evaluated in this study; however, it was noticeable that cells that adhered onto PLA films have shown an increasing proliferation trend with their respective time in culture, that is, greater number of cells in the longer periods in growth medium.…”

Section: Resultsmentioning

confidence: 92%

Biocompatibility Assessment of Poly(lactic acid) Films after Sterilization with Ethylene Oxide in Histological Study In Vivo with Wistar Rats and Cellular Adhesion of Fibroblasts In Vitro

Savaris

Braga

Santos

et al. 2017

International Journal of Polymer Science

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Biomaterials must meet certain fundamental requirements for their usage in living beings, such as biocompatibility, bifunctionality, and sterilizability, without having chemical and structural changes. The biocompatibility of poly(lactic acid) (PLA) films, shaped by compression, was evaluated after sterilization by ethylene oxide by a histological in vivo test with Wistar rats and cytotoxicity in cell adhesion in vitro. The cytotoxicity test was performed by the reduction of tetrazolium salt (MTT). Thermal and chemical changes in PLA films concerning the proposed sterilization process and characteristics were not observed to evidence polymer degradation due to sterilization. The analysis of the cytotoxicity by the MTT method has shown that the sterilized PLA films are not cytotoxic. The adhesion and proliferation of fibroblasts on PLA films were homogeneously distributed over the evaluation period, showing an elongated appearance with unnumbered cytoplasmic extensions and cell-cell interactions. By examining the biocompatibility in a histological study, a mild tissue inflammation was observed with the presence of fibrosis in the samples that had been exposed for 21 days in the rats' bodies. PLA films sterilized with ethylene oxide did not exhibit cell adhesion in vitro and toxicity to the surrounding tissue in vivo and they may be used in future in vivo testing, according to histological findings in Wistar rats in the present study.

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“…This is due to the presence of the OH group of the N-acetylglucosamine moiety of chitosan. Many studies have reported the relationship between cell adhesion and the wettability of a material's surface 12,13) . Generally, cell adhesion was enhanced on the surface with a contact angle between 60-70°.…”

Section: Discussionmentioning

confidence: 99%

Transparent Film Formation of DNA/Cationic Polymer Complexes by Hydrothermal Hot Pressing: Observation of Cell Culture on Films and Biodegradation of Films In Vivo

Irie

Ohno

Hayakawa

et al. 2013

J. Hard Tissue Biology.

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Films were prepared from the powders of DNA/poly-L-arginine, DNA/poly-L-lysine, and DNA/ chitosan complexes via hydrothermal hot-pressing in an effort to make more useful dental materials. The contact angles of water were between 37.6° and 49.9°. Zeta potentials at pH 7.4 were between 10.96 mV and 30.44 mV. The DNA/chitosan complex film showed the lowest contact angle and highest zeta potential. All films showed poor attachment of dermal fibroblast cells, with cells forming spheroids on all films. However, the cells survived on the films after a 5-day cultivation period. The DNA/poly-L-arginine and DNA/poly-L-lysine complex films were almost completely biodegraded within 14 days and most of the DNA/chitosan complex films were biodegraded 90 days after implantation in soft tissues of rats. These results suggest that films formed from DNA/polycation complexes will be useful for clinical treatments requiring thin membranes or films, such as protective membranes for stomatitis and incised oral wounds.

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Adhesion and proliferation of cells on new polymers modified biomaterials

Cited by 85 publications

References 41 publications

Biological and Chemical Assessment of DNA/Chitosan Complex Film

Biological and Chemical Assessment of DNA/Chitosan Complex Film

Biocompatibility Assessment of Poly(lactic acid) Films after Sterilization with Ethylene Oxide in Histological Study In Vivo with Wistar Rats and Cellular Adhesion of Fibroblasts In Vitro

Transparent Film Formation of DNA/Cationic Polymer Complexes by Hydrothermal Hot Pressing: Observation of Cell Culture on Films and Biodegradation of Films In Vivo

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