Adhesion and proliferation of cultured human aortic smooth muscle cells on polystyrene implanted with N+, F+ and Ar+ ions: correlation with polymer surface polarity and carbonization

Bačáková, Lucie; Švorčı́k, Václav; Rybka, V.; Miček, Ivan; Hnatowicz, V.; Vaccari, Lisa; Kocourek, František

doi:10.1016/0142-9612(96)85914-x

Cited by 54 publications

(26 citation statements)

References 19 publications

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“…The structures formed in the polymer by ion implantation are similar to those formed by pyrolysis, but they are found only in the surface layers of the polymer instead of in the entire bulk of the material [47]. For biomedical applications, the ions used are either those of noble gasses, which are assumed to be biologically inert, or ions of elements such as oxygen or nitrogen, which can be commonly found in biomolecules [48,49]. For specific applications, ions of cytotoxic elements such as silver can be used to create surface where no cell adhesion takes place [50].…”

Section: Ion Implantationmentioning

confidence: 99%

Surface Modification of Polymer Substrates for Biomedical Applications

2017

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While polymers are widely utilized materials in the biomedical industry, they are rarely used in an unmodified state. Some kind of a surface treatment is often necessary to achieve properties suitable for specific applications. There are multiple methods of surface treatment, each with their own pros and cons, such as plasma and laser treatment, UV lamp modification, etching, grafting, metallization, ion sputtering and others. An appropriate treatment can change the physico-chemical properties of the surface of a polymer in a way that makes it attractive for a variety of biological compounds, or, on the contrary, makes the polymer exhibit antibacterial or cytotoxic properties, thus making the polymer usable in a variety of biomedical applications. This review examines four popular methods of polymer surface modification: laser treatment, ion implantation, plasma treatment and nanoparticle grafting. Surface treatment-induced changes of the physico-chemical properties, morphology, chemical composition and biocompatibility of a variety of polymer substrates are studied. Relevant biological methods are used to determine the influence of various surface treatments and grafting processes on the biocompatibility of the new surfaces—mammalian cell adhesion and proliferation is studied as well as other potential applications of the surface-treated polymer substrates in the biomedical industry.

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Section: Ion Implantationmentioning

confidence: 99%

Surface Modification of Polymer Substrates for Biomedical Applications

2017

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“…These materials not only serve as growth supports for cell cultures in vitro , but can also be used for constructing replacements for various tissues or organs, e.g. , non-resorbable or semi-resorbable vascular prostheses, artificial heart valves, bone and joint replacements, and implants for plastic surgery (for reviews, see [1–3]).…”

Section: Introductionmentioning

confidence: 99%

“…For this purpose, the surfaces have been exposed to ultraviolet (UV) irradiation [6,7], to a beam of various ions ( e.g. , oxygen, nitrogen, noble gases or halogens for biological applications [1–3,8]) or to a plasma discharge [9–12]. For more pronounced changes in the physicochemical properties of the modified surface, some of these processes can be realised in a gas atmosphere, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Improved Adhesion, Growth and Maturation of Vascular Smooth Muscle Cells on Polyethylene Grafted with Bioactive Molecules and Carbon Particles

Pařízek

Kasálková

Bačáková

et al. 2009

IJMS

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High-density polyethylene (PE) foils were modified by an Ar+ plasma discharge and subsequent grafting with biomolecules, namely glycine (Gly), polyethylene glycol (PEG), bovine serum albumin (BSA), colloidal carbon particles (C) or BSA and C (BSA + C). As revealed by atomic force microscopy (AFM), goniometry and Rutherford Backscattering Spectroscopy (RBS), the surface chemical structure and surface morphology of PE changed dramatically after plasma treatment. The contact angle decreased for the samples treated by plasma, mainly in relation to the formation of oxygen structures during plasma irradiation. A further decrease in the contact angle was obvious after glycine and PEG grafting. The increase in oxygen concentration after glycine and PEG grafting proved that the two molecules were chemically linked to the plasma-activated surface. Plasma treatment led to ablation of the PE surface layer, thus the surface morphology was changed and the surface roughness was increased. The materials were then seeded with vascular smooth muscle cells (VSMC) derived from rat aorta and incubated in a DMEM medium with fetal bovine serum. Generally, the cells adhered and grew better on modified rather than on unmodified PE samples. Immunofluorescence showed that focal adhesion plaques containing talin, vinculin and paxillin were most apparent in cells on PE grafted with PEG or BSA + C, and the fibres containing α-actin, β-actin or SM1 and SM2 myosins were thicker, more numerous and more brightly stained in the cells on all modified PE samples than on pristine PE. An enzyme-linked immunosorbent assay (ELISA) revealed increased concentrations of focal adhesion proteins talin and vinculin and also a cytoskeletal protein β-actin in cells on PE modified with BSA + C. A contractile protein α-actin was increased in cells on PE grafted with PEG or Gly. These results showed that PE activated with plasma and subsequently grafted with bioactive molecules and colloidal C particles, especially with PEG and BSA + C, promotes the adhesion, proliferation and phenotypic maturation of VSMC.

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“…2,3,6 Implantation of Mg 2+ ions to alumina ceramics enhances the differentiation and metabolic activity of human bone-derived cells adhering to these materials. 26 We previously found that polystyrene, polyethylene, and polypropylene after implantation with F + , Ar + , and N + ions (energy 150 keV, 5 ×10 12 or 5 ×10 14 ions/cm 2 ) improved the initial adhesion of human aortic smooth muscle cells. 25,26 At the lower ion dose, the better adhesion of cells was attributed to the increased polarity of the polymer surface due to oxidation and formation of polar groups (e.g., −OH, =O).…”

Section: Introductionmentioning

confidence: 94%

Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture

Bačáková

Mareš

Bottone

et al. 2000

J. Biomed. Mater. Res.

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Vascular smooth muscle cells derived from the rat aorta were cultured on unmodified or F(+) ion-implanted polystyrene (5 x 10(12) or 5 x 10(14) ions/cm(2), energy 150 keV). In 1-day-old cultures, the cells adhered to the modified polystyrene in higher numbers and over larger contact areas. Increased resistance of the cells to trypsin-mediated detachment from the growth support indicated an improved adhesion of cells to the modified polymer at later culture intervals. The cells cultured on ion-modified polymers also were larger and had a higher total protein content. By use of immunocytochemistry, several specific protein species were increased, including the cytoskeletal alpha-actin and vimentin and the plasma membrane-associated vinculin, talin, alpha-v integrins, ICAM-1, and VCAM-1, which account for stronger cell-cell and cell-extracellular matrix adhesion. The lower number of cells found floating in the medium suggests that the spontaneous detachment of cells from the modified polystyrene was lower and that the viability of the adhered cell population was higher. As was shown by the two-parameter flow-cytometric measurements of BrdU incorporation and DNA content, as well as by (3)H-thymidine autoradiography, the cell proliferation on samples modified by the dose of 5 x 10(12) ions/cm(2) was similar to that in controls; and at the dose of 5 x 10(14) ions/cm(2), it tended to be even lower. The cells grown on the polymer implanted with the dose of 5 x 10(12) ions/cm(2) responded to a new artificially created cell-free area in a confluent cell layer by more intense migration whereas at the dose of 5 x 10(14) ions/cm(2), the migration ability of cells was similar to that on the unmodified polymer. The data revealed a higher biocompatibility of ion-implanted polystyrene with vascular smooth muscle cells in culture. There was better adhesion, differentiation, and survival, and there was neither excessive migration nor proliferation.

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Adhesion and proliferation of cultured human aortic smooth muscle cells on polystyrene implanted with N+, F+ and Ar+ ions: correlation with polymer surface polarity and carbonization

Cited by 54 publications

References 19 publications

Surface Modification of Polymer Substrates for Biomedical Applications

Surface Modification of Polymer Substrates for Biomedical Applications

Improved Adhesion, Growth and Maturation of Vascular Smooth Muscle Cells on Polyethylene Grafted with Bioactive Molecules and Carbon Particles

Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture

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