Improved Adhesion, Growth and Maturation of Vascular Smooth Muscle Cells on Polyethylene Grafted with Bioactive Molecules and Carbon Particles

Pařízek, Martin; Kasálková, Nikola Slepičková; Bačáková, Lucie; Slepička, Petr; Vaccari, Lisa; Blažková, Martina; Švorčı́k, Václav

doi:10.3390/ijms10104352

Cited by 33 publications

(36 citation statements)

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“…For example, silver nanoparticles have antimicrobial properties and are thus often utilized on substrates where the interaction with the biological environment is undesirable [97], while gold nanoparticles rarely induce an allergic response and are believed to not be cytotoxic. Gold nanoparticles furthermore exhibit good biocompatible properties and are thus a good candidate for various biomedical applications in immunology, nanomedicine and biotechnology [98,99]. Carbon-based nanoparticles are also an interesting option as there are several types of nanoscale carbon structures with promising biomedical applications due to their unique physico-chemical properties.…”

Section: Nanoparticle Graftingmentioning

confidence: 99%

Surface Modification of Polymer Substrates for Biomedical Applications

2017

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While polymers are widely utilized materials in the biomedical industry, they are rarely used in an unmodified state. Some kind of a surface treatment is often necessary to achieve properties suitable for specific applications. There are multiple methods of surface treatment, each with their own pros and cons, such as plasma and laser treatment, UV lamp modification, etching, grafting, metallization, ion sputtering and others. An appropriate treatment can change the physico-chemical properties of the surface of a polymer in a way that makes it attractive for a variety of biological compounds, or, on the contrary, makes the polymer exhibit antibacterial or cytotoxic properties, thus making the polymer usable in a variety of biomedical applications. This review examines four popular methods of polymer surface modification: laser treatment, ion implantation, plasma treatment and nanoparticle grafting. Surface treatment-induced changes of the physico-chemical properties, morphology, chemical composition and biocompatibility of a variety of polymer substrates are studied. Relevant biological methods are used to determine the influence of various surface treatments and grafting processes on the biocompatibility of the new surfaces—mammalian cell adhesion and proliferation is studied as well as other potential applications of the surface-treated polymer substrates in the biomedical industry.

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Section: Nanoparticle Graftingmentioning

confidence: 99%

Surface Modification of Polymer Substrates for Biomedical Applications

2017

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“…The grafting of this molecule on the PE surface leads to the increase in oxygen concentration on the modified surface and decrease in WCA. Combination with other surface properties (morphology and roughness) leads grafting with Gly to an increase in the attractiveness of PE for vascular smooth muscle cell (VSMC) colonization [107].…”

Section: Wettability Of Grafted Polymer Surface and Cytocompatibilitymentioning

confidence: 99%

Wettability and Other Surface Properties of Modified Polymers

Kasálková¹,

Slepička²,

Kolská³

et al. 2015

Wetting and Wettability

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Surface wettability is one of the crucial characteristics for determining of a material's use in specific application. Determination of wettability is based on the measurement of the material surface contact angle. Contact angle is the main parameter that characterizes the drop shape on the solid surface and is also one of the directly measurable properties of the phase interface. In this chapter, the wettability and its related properties of pristine and modified polymer foils will be described. The wettability depends on surface roughness and chemical composition. Changes of these parameters can adjust the values of contact angle and, therefore, wettability. In the case of pristine polymer materials, their wettability is unsuitable for a wide range of applications (such as tissue engineering, printing, and coating). Polymer surfaces can easily be modified by, e.g., plasma discharge, whereas the bulk properties remain unchanged. This modification leads to oxidation of the treated layer and creation of new chemical groups that mainly contain oxygen. Immediately after plasma treatment, the values of the contact angles of the modified polymer significantly decrease.In the case of a specific polymer, the strongly hydrophilic surface is created and leads to total spreading of the water drop. Wettability is strongly dependent on time from modification.Wettability plays a key role, e.g., in the development of biomaterials in tissue engineering and regenerative medicine. Biocompatibility tests of the cell adhesion, proliferation and viability are performed in an aqueous medium, and it is necessary to control the surface wettability. Various cell types have different requirements on surface properties, but while maintaining suitable parameters, the optimal value of © 2015 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.water contact angle for cell adhesion is in the interval of 50-70°. In the case of polymers, which have usually higher values of contact angles, the decrease in water contact angle and adjustment of the surface may be accomplished by introducing selected chemical groups (by plasma treatment), chemical compounds (by grafting, i.e., chemical bath deposition), or nanoparticles. In the case of grafting of polyethylene glycol (PEG) on the plasma activated high-density polyethylene was under "properly" selected conditions of modification (molecular weight of PEG, concentration of PEG in solution) prepared layers that positively influenced the cell adhesion. In addition, low concentration of gold nanoparticles increased the number of adhered cells without decreasing cell viability.Whether the surface of the polymeric substrate is attractive for the adhesion and proliferation of cells is determined not only by wettability but also by a range of other surface properties...

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“…In order to further improve the attractiveness of cellulose-based materials for cell colonization, not only modification with chitosan and arginine but also other modifications can be considered. These modifications involve plasma, ion or UV irradiation, which are proved to increase the attractiveness of synthetic polymers for cell adhesion and growth (Parizek et al 2009; for a review, see Bacakova et al 2011). Plasma modification increased the adsorption of chitosan to cellulose (Fras Zemljic et al 2009).…”

Section: Phenotypic Maturation Of Vsmc On Cellulosebased Materialsmentioning

confidence: 99%

Cellulose-based materials as scaffolds for tissue engineering

et al. 2013

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Two types of cellulose-based materials, 6-carboxycellulose with 2.1 or 6.6 wt% of -COOH groups, were prepared and tested for potential use in tissue engineering. The materials were functionalized with arginine, i.e. an amino acid with a basic side chain, or with chitosan, in order to balance the relatively acid character of oxidized cellulose molecules, and were seeded with vascular smooth muscle cells (VSMC). The cell adhesion and growth were then evaluated directly on the materials, and also on the underlying polystyrene culture dishes. Of these two types of studied materials, 6-carboxycellulose with 2.1 wt% of -COOH groups was more appropriate for cell colonization. The cells on this material achieved an elongated shape, while they were spherical in shape on the other materials. The number of cells and the concentration (per mg of protein) of contractile proteins alpha-actin and SM1 and SM2 myosins, i.e. markers of the phenotypic maturation of VSMC, were also significantly higher on this material. Functionalization of the material with arginine and chitosan further improved the phenotypic maturation of VSMC. Chitosan also improved the adhesion and growth of these cells. In comparison with the control polystyrene dishes, the proliferation of cells on our cellulose-based materials was relatively low. This suggests that these materials can be used in applications where high proliferation activity of cells is not desirable, e.g. proliferation of VSMC on vascular prostheses. Alternatively, the cell proliferation might be enhanced by another more efficient modification, which would require further research.

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Improved Adhesion, Growth and Maturation of Vascular Smooth Muscle Cells on Polyethylene Grafted with Bioactive Molecules and Carbon Particles

Cited by 33 publications

References 36 publications

Surface Modification of Polymer Substrates for Biomedical Applications

Surface Modification of Polymer Substrates for Biomedical Applications

Wettability and Other Surface Properties of Modified Polymers

Cellulose-based materials as scaffolds for tissue engineering

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