Adhesion-based simple capture and recovery of circulating tumor cells using a blood-compatible and thermo-responsive polymer-coated substrate

Hoshiba, Takashi; Orui, Toshihiko; Endo, Chiho; Sato, Kazuhiro; Yoshihiro, Ayano; Minagawa, Yasuhisa; Tanaka, Masaru

doi:10.1039/c6ra15229e

Cited by 25 publications

(24 citation statements)

References 34 publications

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“…Thin films of the polymers were prepared by spin-coating 0.2 wt/vol % methanol solutions twice onto polyethylene terephthalate (PET) substrates. The polymer film is retained due to the van der Waals interaction between polymer and substrate, and the thickness is known to be around 100 nm by transmission electron microscopy . The films were placed in PBS and observed by AFM using a Cypher (Oxford Inst.…”

Section: Methodsmentioning

confidence: 99%

“…The polymer film is retained due to the van der Waals interaction between polymer and substrate, and the thickness is known to be around 100 nm by transmission electron microscopy. 25 The films were placed in PBS and observed by AFM using a Cypher (Oxford Inst. Inc.) equipped with an exact temperature controller.…”

Section: Determination Of the Lcst Of Pme2ma Aqueous Solutionmentioning

confidence: 99%

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Thermosensitive Polymer Biocompatibility Based on Interfacial Structure at Biointerface

Murakami¹,

Kitahara

Kobayashi

et al. 2018

ACS Biomater. Sci. Eng.

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The interfacial structure of a thermosensitive biocompatible polymer, poly[2-(2-methoxyethoxy)ethyl methacrylate] (PMe2MA), at the polymer/phosphate-buffered saline (PBS) interface was investigated by atomic force microscopy. A number of nanometer scale protrusions appeared at 37 °C and disappeared at 22 °C, reversibly. This structural change occurred above the lower critical solution temperature of PMe2MA in PBS (19 °C), indicating that the formation of protrusions was explained by the microphase separation of polymer and water at the interfacial region. The protein adsorption and platelet adhesion onto PMe2MA interface were drastically restrained at 22 °C compared to that at 37 °C. Detachment of NIH3T3 cells accompanied by the dissipation of protrusions on the PMe2MA interface was also demonstrated. These results indicate that the protrusions act as the scaffold for the protein adsorption and cell adhesion.

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Section: Methodsmentioning

confidence: 99%

Section: Determination Of the Lcst Of Pme2ma Aqueous Solutionmentioning

confidence: 99%

Thermosensitive Polymer Biocompatibility Based on Interfacial Structure at Biointerface

Murakami¹,

Kitahara

Kobayashi

et al. 2018

ACS Biomater. Sci. Eng.

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“…We have reported by TEM observation that the thickness of the PMEA film in the present conditions was around 100 nm. 78 Hence, the structure appeared only at the top layer region of the PMEA film. The protrusions were stable in water at least one 1 day.…”

Section: Observation Of Pmea/air and Pmea/water Interfacesmentioning

confidence: 99%

Design of Polymeric Biomaterials: The “Intermediate Water Concept”

Tanaka

Kobayashi

Murakami

et al. 2019

Bulletin of the Chemical Society of Japan

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During this period, he received a fellowship from the Japan Society for the Promotion of Science (JSPS Postdoctoral Fellowships). He worked as a researcher at Japan Science and Technology Agency (JAT) ERATO Project and Kyushu Synchrotron Light Research Center. He moved to Kyushu University as an Assistant Professor in 2015. His current research activities are concerned with the analysis of structure, properties, and hydration state of biomaterial interfaces.

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“…The intermediate water is also observed in biocompatible/inert/non-fouling synthetic polymers ( Figure 1 d) [ 83 , 84 , 85 ]. The intermediate water dictates the cells behavior such as cellular attachment, proliferation, migration, and differentiation [ 86 , 87 , 88 ], and can be considered as a potential parameter for designing biomaterial scaffolds.…”

Section: Importance Of Smart Biomaterials In Tementioning

confidence: 99%

Designing Smart Biomaterials for Tissue Engineering

Khan

Tanaka

2017

IJMS

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220

129

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The engineering of human tissues to cure diseases is an interdisciplinary and a very attractive field of research both in academia and the biotechnology industrial sector. Three-dimensional (3D) biomaterial scaffolds can play a critical role in the development of new tissue morphogenesis via interacting with human cells. Although simple polymeric biomaterials can provide mechanical and physical properties required for tissue development, insufficient biomimetic property and lack of interactions with human progenitor cells remain problematic for the promotion of functional tissue formation. Therefore, the developments of advanced functional biomaterials that respond to stimulus could be the next choice to generate smart 3D biomimetic scaffolds, actively interacting with human stem cells and progenitors along with structural integrity to form functional tissue within a short period. To date, smart biomaterials are designed to interact with biological systems for a wide range of biomedical applications, from the delivery of bioactive molecules and cell adhesion mediators to cellular functioning for the engineering of functional tissues to treat diseases.

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Adhesion-based simple capture and recovery of circulating tumor cells using a blood-compatible and thermo-responsive polymer-coated substrate

Abstract: Circulating tumor cells (CTCs) have been a focus of study for metastatic cancer diagnostics, in in vitro anti-cancer drug screening to decide the chemotherapeutic course, and cancer biology research.

Cited by 25 publications

References 34 publications

Thermosensitive Polymer Biocompatibility Based on Interfacial Structure at Biointerface

Thermosensitive Polymer Biocompatibility Based on Interfacial Structure at Biointerface

Design of Polymeric Biomaterials: The “Intermediate Water Concept”

Designing Smart Biomaterials for Tissue Engineering

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