Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interactions

Abstract

Thirteen human bifidobacterial strains were tested for their abilities to adhere to human enterocyte-like Caco-2 cells in culture. The adhering strains were also tested for binding to the mucus produced by the human mucus-secreting HT29-MTX cell line in culture. A high level of calcium-independent adherence was observed for Bifidobacterium breve 4, for Bifidobacterium infantis 1, and for three fresh human isolates from adults. As observed b3 scanning electron microscopy, adhesion occurs to the apical brush border of the enterocytic Caco-2 cells and to the mucus secreted by the HT29-MTfX mucus-secreting cells. The bacteria interacted with the well-defined apical microvilli of Caco-2 cells without cell damage. The adhesion to Caco-2 cells of bifidobacteria did not require calcium and was mediated by a proteinaceous adhesion-promoting factor which was present both in the bacterial whole cells and in the spent supernatant of bifidobacterium culture. This adhesion. promoting factor appeared species specific, as are the adhesion-promoting factors of lactobacilli. We investigated the inhibitory effect of adhering human bifidobacterial strains against intestinal cell monolayer colonization by a variety of diarrheagenic bacteria. B. breve 4, B. infantis 1, and fresh human isolates were shown to inhibit cell association of enterotoxigenic, enteropathogenic, diffusely adhering Escherichia coli and Salmonela typhimurium strains to enterocytic Caco-2 cells in a concentration-dependent manner. Moreover, B. breve 4 and B. infantis 1 strains inhibited, dose dependently, Caco-2 cell invasion by enteropathogenic E. coli, Yersinia pseudotuberculosis, and S. typhimurium strains.

Influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota

“…That is to say, delayed colonization of bifidobacteria appeared to lead to the unusual growth of Enterobacteriaceae in the AT subjects. This is consistent with the data in some previous studies that showed an antagonistic relationship between Bifidobacteria and Enterobacteriaceae (Bernet et al, 1993;Gibson & Wang, 1994;Hutt et al, 2006). It is interesting that the development of gastrointestinaltract microbiota in the CD infants followed similar, although weaker, trend to AT infants, namely, less bacterial diversity in the first week, delayed colonization of bifidobacteria, and overgrowth of Enterobacteriaceae.…”

In vitro antibacterial activity of Lactobacillus helveticus strain KS300 against diarrhoeagenic, uropathogenic and vaginosis-associated bacteria

“…1999). It is interesting to note that the pattern of killing activity displayed by L. helveticus strain KS300 against S. typhimurium resembles the patterns of killing activity previously described for probiotic Lactobacillus strains including Lactobacillus johnsonii La1, L. rhamnosus GG, L. rhamnosus strain GR-1, Lactobacillus casei Shirota strain YT9029, and L. casei strain DN-114 001 (Bernet et al 1993;Hudault et al 1997;Fayol-Messaoudi et al 2005), and Lactobacillus acidophilus strain LB (Coconnier et al 1997). These Lactobacillus strains killed S. typhimurium mainly by the action of secreted nonlactic acid molecules present in CFCSs (Silva et al 1987;McGroarty and Reid 1988;Bernet-Camard et al 1997;Coconnier et al 1997;Fayol-Messaoudi et al 2005).…”

Effects of probiotics, lactitol and rifaximin on intestinal flora and fecal excretion of organic acids in cirrhotic patients

“…16,18 Bifidobacterium and Lactobacillus have been long known to produce bacteriocines and unfavorable adhesion conditions for Gram-positive species. 19,20 In the present study, all three tested compounds produced a reduction in the Gram-positive population in favor of an expansion of Bifidobacterium and Lactobacillus. However, the specific bacterial count of Bacteroides and Clostridium significantly decreased only in the group treated with the synbiotic.…”