Adhesion of human periodontal ligament cells by three-dimensional culture to the sterilized root surface of extracted human teeth

Abstract

Residual periodontal ligament (PDL)and cement mass on the roots of extracted teeth are factors that considerably affect tooth transplantation. Therefore, when normal extracted teeth are used for autologous transplantation, it is necessary to regenerate the PDL of the root surface. Here we describe a method to examine human PDL cell adhesion on sterilized root surfaces. Sample teeth were extracted during orthodontic treatment. PDL cells were obtained from healthy periodontal tissue explants from teeth extracted for orthodontic reasons. We developed a method for adhering PDL cells to sterile root surfaces using three-dimensional culture for 3 weeks. We evaluated the adhesion of human PDL cells to the sterilized root surfaces biochemically and histologically. The adherent PDL cells presented new projections on the sterile root surfaces. Therefore, PDL cells can adhere to sterile root surfaces.

Autotransplantation of the Third Molar: A Therapeutic Alternative to the Rehabilitation of a Missing Tooth: A Scoping Review

“…One of the main reasons for the loss of the autograft tooth is the damage caused to the periodontal apparatus during surgical maneuvers, which subsequently leads to root resorption. Some studies report that the third molar could potentially be used as a scaffold for tissue regeneration of the periodontal ligament; in fact, Mino et al evaluated the adhesion of HPDL cells (human periodontal ligament) on the root surfaces in order to regenerate the periodontal membrane on a sterile root surface in vitro before transplantation [ 77 ], obtaining the adhesion of the periodontal ligament cells on the tooth surface sterilized. A bioabsorbable polymeric material 3D printed after design and transplanted with HPDL cells was also used as scaffold material for periodontal regeneration [ 78 ].…”