Adhesion of mammalian cells to polymer surfaces: from physical chemistry of surfaces to selective adhesion on defined patterns

Dewez, Jean‐Luc; Lhoest, Jacques; Detrait, Eric; Berger, Valérie; Dupont‐Gillain, Christine C.; Vincent, Luc; Schneider, Yves‐Jacques; Bertrand, Patrick; Rouxhet, Paul

doi:10.1016/s0142-9612(98)00055-6

Cited by 121 publications

(88 citation statements)

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“…They use an ion beam/ plasma instead of ultraviolet light to conduct the lithography. Dewez et al have reported the adhesion of some mammalian cells, such as human epithelial cells, PC12 pheochromocytoma, MSC80 schwannonoma, Hep G2 hepatoblastoma, and rat hepatocytes, on patterned (produced by photolithography and oxygen plasma treatment) polystyrene surfaces having tracks (width in the range of a few tens of mm) with reduced hydrophobicity [100]. Kapur et al also indicate that some selectively patterned three-dimensional polymer substrates may be useful in a variety of biomaterial applications [101].…”

Section: Surface Morphologymentioning

confidence: 99%

Plasma-surface modification of biomaterials

Chu

Chen

Wang

et al. 2002

Materials Science and Engineering: R: Reports

1,420

652

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Plasma-surface modification (PSM) is an effective and economical surface treatment technique for many materials and of growing interests in biomedical engineering. This article reviews the various common plasma techniques and experimental methods as applied to biomedical materials research, such as plasma sputtering and etching, plasma implantation, plasma deposition, plasma polymerization, laser plasma deposition, plasma spraying, and so on. The unique advantage of plasma modification is that the surface properties and biocompatibility can be enhanced selectively while the bulk attributes of the materials remain unchanged. Existing materials can, thus, be used and needs for new classes of materials may be obviated thereby shortening the time to develop novel and better biomedical devices. Recent work has spurred a number of very interesting applications in the biomedical field. This review article concentrates upon the current status of these techniques, new applications, and achievements pertaining to biomedical materials research. Examples described include hard tissue replacements, blood contacting prostheses, ophthalmic devices, and other products. #

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Section: Surface Morphologymentioning

confidence: 99%

Plasma-surface modification of biomaterials

Chu

Chen

Wang

et al. 2002

Materials Science and Engineering: R: Reports

1,420

652

View full text Add to dashboard Cite

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“…As expected, hepatocyte morphology is influenced by the shape of the adhesive collagen patterns: hepatocytes on narrow collagen strips display elongated shapes. 18,37,260,261 Singhvi et al 262 cultured hepatocytes on micropatterns of laminin absorbed onto microstamped SAM patterns (laminin on alkanethiol SAMs, with a nonadhesive HEG-SAM) and found that limiting the degree of cell extension by decreasing the size of the adhesive islands resulted in a progressive reduction of growth and increase of albumin secretion. Hepatocytes cultured on unpatterned substrata rapidly lost the ability to secrete high levels of albumin, while hepatocytes maintained near-normal levels of albumin secretion for at least 3 days when cultured on the smallest adhesive islands that fully restrict cell extension.…”

Section: Vf Liver Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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“…In particular, hydrophilization of polymers by oxygen or nitrogen plasmas has found wide use. In biomedical applications, plasma hydrophilization can improve the biocompatibility of polymers [1][2][3] as well as affect the attachment density of cells [4][5][6][7] and the adsorption of proteins. 8,9 Capillary filling of polymer microfluidic channels [10][11][12][13] and structured substrates 9,14,15 is also enhanced by hydrophilization, since most polymers in their native form are either hydrophobic or only slightly hydrophilic.…”

Section: Introductionmentioning

confidence: 99%

Oxygen and nitrogen plasma hydrophilization and hydrophobic recovery of polymers

Jokinen

Suvanto²,

Franssila³

2012

Biomicrofluidics

168

118

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Plasma hydrophilization and subsequent hydrophobic recovery are studied for ten different polymers of microfabrication interest: polydimethylsiloxane (PDMS), polymethylmethacrylate, polycarbonate, polyethylene, polypropylene, polystyrene, epoxy polymer SU-8, hybrid polymer ORMOCOMP, polycaprolactone, and polycaprolactone/D,L-lactide (P(CL/DLLA)). All polymers are treated identically with oxygen and nitrogen plasmas, in order to make comparisons between polymers as easy as possible. The primary measured parameter is the contact angle, which was measured on all polymers for more than 100 days in order to determine the kinetics of the hydrophobic recovery for both dry stored and rewashed samples. Clear differences and trends are observed both between different polymers and between different plasma parameters.

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Adhesion of mammalian cells to polymer surfaces: from physical chemistry of surfaces to selective adhesion on defined patterns

Cited by 121 publications

References 0 publications

Plasma-surface modification of biomaterials

Plasma-surface modification of biomaterials

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Oxygen and nitrogen plasma hydrophilization and hydrophobic recovery of polymers

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